Pseudomonas alcaliphila NEWG-2 as biosorbent agent for methylene blue dye: optimization, equilibrium isotherms, and kinetic processes

Abstract

In comparison to physicochemical and chemical methods, microbial dye biosorption is regarded as an eco-effective and economically viable alternative and is a widely applied method due to its high efficiency and compatibility with the environment. Therefore, the idea of this study is to clarify to what extent the viable cells and the dry biomass of Pseudomonas alcaliphila NEWG-2 can improve the biosorption of methylene blue (MB) from a synthetic wastewater sample. The array of Taguchi paradigm has been conducted to ascertain five variables affecting the biosorption of MB by broth forms of P. alcaliphila NEWG. The data of MB biosorption were familiar to the predicted ones, indicating the precision of the Taguchi model’s prediction. The maximum biosorption of MB (87.14%) was achieved at pH 8, after 60 h, in a medium containing 15 mg/ml MB, 2.5% glucose, and 2% peptone, with sorting the highest signal-to-noise ratio (38.80). FTIR spectra detected various functional groups (primary alcohol, α, β-unsaturated ester, symmetric NH2 bending, and strong C–O stretching) on the bacterial cell wall that participated in the biosorption of MB. Furthermore, the spectacular MB biosorption ability was validated by equilibrium isotherms and kinetic studies (the dry biomass form), which were derived from the Langmuir model (qmax = 68.827 mg/g). The equilibrium time was achieved in about 60 min, with 70.5% of MB removal. The biosorption kinetic profile might be adequately represented by pseudo-second order and Elovich models. The changes in the bacterial cells before and after the biosorption of MB were characterized using a scanning electron microscope. As realized from the aforementioned data, the bacterium is a talented, effective, eco-friendly, and low-cost bio-sorbent for the decolorization and remedy of an industrial effluent containing MB from an aqueous environment. The current outcomes in the biosorption of MB molecules promote the use of the bacterial strain as viable cells and/or dry biomass in ecosystem restoration, environmental cleanup, and bioremediation studies.

A Comparative Study of Cr(VI) Sorption by Aureobasidium pullulans AKW Biomass and Its Extracellular Melanin: Complementary Modeling with Equilibrium Isotherms, Kinetic Studies, and Decision Tree Modeling

“…As a result, the population can be divided into two or more homogeneous groups. These homogeneous sets are built using the most substantial differentiator on the input variables [ 27 , 80 ]. No information is available about the combination of DT with equilibrium isotherms and kinetic studies for the determination of relative variable importance and modeling the biosorption process.…”

Biodiesel Production through Rhodotorula toruloides Lipids and Utilization of De-Oiled Biomass for Congo Red Removal

“…The high correlation coefficients (R 2 = 0.977) indicated that the Langmuir model explains the Congo red biosorption equilibrium by the de-oiled biomass in the tested concentration ranges. It was stated that the Langmuir isotherm depends on the hypothesis of monolayer coverage of the tested dye onto a biosorbent with the homogenous surface, i.e., the biosorbent consists of similar sites with equal binding energy [91].…”