Pseudochrobactrum glaciei sp. nov., isolated from sea ice collected from Peter the Great Bay of the Sea of Japan

Romanenko, Lyudmila A.; Tanaka, Naoto; Frolova, Galina M.; Mikhailov, Valery V.

doi:10.1099/ijs.0.65828-0

Cited by 22 publications

(10 citation statements)

References 24 publications

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“…The genus Pseudochrobactrum was proposed by Kämpfer et al (2006), and at the time of writing comprises four species, Pseudochrobactrum saccharolyticum, Pseudochrobactrum asaccharolyticum, Pseudochrobactrum kiredjianiae, Pseudochrobactrum lubricantis (Kämpfer et al, 2006(Kämpfer et al, , 2007(Kämpfer et al, , 2009). The genus Paenochrobactrum was proposed by Kämpfer et al (2010) with two species, Paenochrobactrum gallinarii as the type species and the reclassification of Pseudochrobactrum glaciei (Romanenko et al, 2008) as Paenochrobactrum glaciei.…”

mentioning

confidence: 99%

Falsochrobactrum ovis gen. nov., sp. nov., isolated from a sheep

Kämpfer

Glaeser

Busse³

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (B1315 T ) was isolated from the placenta of a sheep with abortion. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the Brucella-OchrobactrumPaenochrobactrum-Pseudochrobactrum group with 94.5-94.8 %, 94.3-96.1 %, 95.0-95.1 %, and 95.9-96.1 % sequence similarities to type strains of species of the four genera, respectively. Phylogenetic trees indicated a close relationship to the type strains of Ochrobactrum gallinifaecis and Ochrobactrum oryzae (95.9 and 96.1 % sequence similarity, respectively). Chemotaxonomic data confirmed the allocation of strain B1315T to the family Brucellaceae (quinone system: ubiquinone Q-10 and major fatty acids: C 18 : 1 v7c and C 19 : 0 cyclo v8c). The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and the unidentified but unique phospholipid PL7. The polyamine pattern of strain B1315 T contained the major polyamines spermidine and putrescine. On the basis of the 16S rRNA gene and recA sequence phylogeny and chemotaxonomic data strain B1315 T was clearly different from the genera Brucella,

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mentioning

confidence: 99%

Falsochrobactrum ovis gen. nov., sp. nov., isolated from a sheep

Kämpfer

Glaeser

Busse³

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…At present, it comprises Pseudochrobactrum saccharolyticum, Pseudochrobactrum asaccharolyticum, Pseudochrobactrum kiredjianiae (Kämpfer et al, 2006(Kämpfer et al, , 2007 and Pseudochrobactum glaciei (Romanenko et al, 2008). Members of the genus can be clearly differentiated from members of the genera Ochrobactrum and Brucella on the basis of 16S rRNA gene sequence and recA sequence data, as well as chemotaxonomic data (Kämpfer et al, 2006(Kämpfer et al, , 2007.…”

mentioning

confidence: 99%

“…Data were obtained in the present study except where indicated. *Data in parentheses are from Romanenko et al (2008). DThe position of the double bond is located by counting from the methyl (v) end of the carbon chain.…”

mentioning

confidence: 99%

Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid

Kämpfer¹,

Huber²,

Lodders³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (KSS 7.8 T ) was isolated from a water-mixed metal-working fluid. On the basis of 16S rRNA gene and recA sequence similarities, the isolate was clearly grouped in the genus Pseudochrobactrum. This allocation was confirmed by fatty acid data (major fatty acids: C 18 : 2 v7c and C 19 : 0 cyclo v8c), polar lipid profile (major components: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine and unknown aminolipid AL1), quinone system (ubiquinone Q-10) and polyamine pattern (spermidine and putrescine predominant). DNA-DNA pairing with the most closely related Pseudochrobactrum species showed values ranging from 24.2 to 45.7 %, and physiological and biochemical data clearly differentiated this isolate from described Pseudochrobactrum species. This organism represents a novel species of the genus Pseudochrobactrum, for which the name Pseudochrobactrum lubricantis sp. nov. is proposed, with the type strain KSS 7.8 T (5CCUG 56963 T 5CCM 7581 T ).The genus Pseudochrobactrum was proposed by Kämpfer et al. (2006). At present, it comprises Pseudochrobactrum saccharolyticum, Pseudochrobactrum asaccharolyticum, Pseudochrobactrum kiredjianiae (Kämpfer et al., 2006(Kämpfer et al., , 2007 and Pseudochrobactum glaciei (Romanenko et al., 2008). Members of the genus can be clearly differentiated from members of the genera Ochrobactrum and Brucella on the basis of 16S rRNA gene sequence and recA sequence data, as well as chemotaxonomic data (Kämpfer et al., 2006(Kämpfer et al., , 2007.Strain KSS 7.8 T was isolated from a metal-working fluid of a metal-processing company in Germany on nutrient agar at 30 u C. Subcultivation was done on tryptone soy agar at 28 uC for 48 h. On this agar, this organism grew also at 15-45 u C, but not at 10 or 50 u C. Growth at 30 u C was also observed on MacConkey agar and R2A agar (all from Oxoid).Gram staining was performed as described by Gerhardt et al. (1994). Cell morphology was observed under a Zeiss light microscope at 61000, with cells grown for 3 days at 30 u C on nutrient agar. The 16S rRNA gene was analysed as described previously (Kämpfer et al., 2003). Sequence analysis and similarity calculations were carried out by using the software programs Bionumerics v. 4.0 (Applied Maths BVBA) and MEGA v. 3.1 (Kumar et al., 2004). The sequenced length of the 16S rRNA gene was 1401 bp (GenBank accession no. FM209496) for strain KSS 7.8 T . Sequence similarities to the three most closely related Pseudochrobactrum species were 99.8 % to P. saccharolyticum CCUG 33852 T , 99.8 % to P. asaccharolyticum CCUG 46016 T and 99.3 % to P. kiredjianiae CCUG 49584 T . A lower similarity of 96.2 % was found to P. glaciei KMM 3858 T . The 16S rRNA gene-based tree shown in Fig. 1 results from a neighbour-joining reconstruction using the Kimura two-parameter correction and 1000 resamplings for bootstrap analysis.The partial r...

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“…recA-Pg-F and recA-Pg-R as described by Romanenko et al (2008). However, while recA sequences from reference strains could be amplified, a recA sequence of strain 280 T could not be amplified.…”

Section: Strain 280mentioning

confidence: 95%

“…At the time of writing it consists of two species, Paenochrobactrum gallinarii and Paenochrobactrum glaciei (Kämpfer et al, 2010). The latter species was originally described as Pseudochrobactrum glaciei (Romanenko et al, 2008). The genus can be clearly differentiated from the genera Ochrobactrum, Pseudochrobactrum and Brucella on the basis of 16S rRNA gene sequence and recA sequence data (phylogenetic analysis), as well as chemotaxonomic data (Kämpfer et al, 2006;2010).…”

mentioning

confidence: 99%

Paenochrobactrum pullorum sp. nov. isolated from a chicken

Kämpfer

Poppel

Wilharm

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain 280 T ) isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus Paenochrobactrum group with a 16S rRNA gene sequence similarity of 98.8 % to the currently recognized species, Paenochrobactrum gallinarii and Paenochrobactrum glaciei. This allocation was confirmed by the fatty acid data (major fatty acids: C 18 : 1 v7c and C 19 : 0 cyclo v8c) and a polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also, the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and the genus-specific 'stretched aminolipid' was well in line with the description of the genus Paenochrobactrum. The quinone system consisted predominantly of ubiquinone Q-10 with traces of Q-9 and Q-11. DNA-DNA hybridization of strain 280 T with Paenochrobactrum

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Pseudochrobactrum glaciei sp. nov., isolated from sea ice collected from Peter the Great Bay of the Sea of Japan

Cited by 22 publications

References 24 publications

Falsochrobactrum ovis gen. nov., sp. nov., isolated from a sheep

Falsochrobactrum ovis gen. nov., sp. nov., isolated from a sheep

Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid

Paenochrobactrum pullorum sp. nov. isolated from a chicken

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