Pru p 7 Predicts Severe Reactions after Ingestion of Peach in Japanese Children and Adolescents

Abstract

<b><i>Introduction:</i></b> Component-resolved diagnostics is used to diagnose food allergies. However, few reports have evaluated the severity of peach fruit allergy using peach allergen components, including Pru p 7. <b><i>Objective:</i></b> This study aimed to predict peach fruit allergy severity based on the presence of specific IgE (sIgE) antibodies (Abs) to peach allergenic components. <b><i>Methods:</i></b> Twenty-seven patients with peach fruit allergy were enrolled and classified into two groups: the local reaction (LR) group, including 12 patients with only oral or throat mucosal symptoms, and the systemic reaction (SR) group, including 15 patients, 10 of whom experienced anaphylaxis. Serum sIgE Abs against crude peach extract – Pru p 1, 2, 3, 4, and 7 – and tree pollen were measured. <b><i>Results:</i></b> sIgE Ab titers of Pru p 1 and 4 and alder pollen in the LR group were significantly higher than those in the SR group. sIgE against Pru p 7 was significantly higher in the SR group than in the LR group. The frequencies of sIgE Abs against Pru p 1, 4, and 7 in the LR group were 91.7, 66.7, and 16.7%, respectively, while in the SR group these were 80, 20, and 60%. Sensitization to Pru p 2 and 3 was detected but limited in all patients. <b><i>Conclusions:</i></b> These findings suggest that sensitization to Pru p 1 and Pru p 4 is associated with local symptoms, and sensitization to Pru p 7 is associated with SR and anaphylaxis. To predict the severity of peach fruit allergy, it is useful to assess sIgE Ab reactions combining Pru p 1, 4, and 7.

Unusual and Unexpected Allergic Reactions Can Be Unraveled by Molecular Allergy Diagnostics

“…They observed exacer-bations or an onset of IBS symptoms and increased numbers of inflammatory cells in the duodenal mucosa during the birch pollen season in individuals with birch pollen allergy. Another recent description of the severe implications of PFS concerns Gibberellin-regulated protein allergy [40,41]. These proteins are found in peach (Pru p 7) and cypress pollen among others.…”

Skin Testing With Peach Peel Extract Versus Serum IgE to Pru p 3 as a Stronger Predictor of Peach-Induced Anaphylaxis

“…Initially reported in Mediterranean countries, 2 3 the leading role has also been reported in Central Europe 4 and elsewhere. 5 6 11 Regarding Pru p 9, a recently recognized allergen, 12 13 it is not present in fruits and therefore does not contribute to sensitization from peach intake. 12 …”

“… 10 Pru p 7 is a gibberellin-regulated protein (7 kDa), associated with anaphylaxis in people negative to Pru p 3. 11 Other allergens like Pru p 9 are involved in respiratory allergy. 12 …”

When the Frequencies of Sensitization and Elicitation of Allergic Reaction Do Not Correlate—The Case of Apple Gibberellin-Regulated Protein Tested in an Italian Population

“…To perform the SPHIAa assay, five characterized sera (numbered 1-5) were selected from the InterAll data bank (version 5.0, ADL) because they had been shown to contain IgE specifically binding the peach peamaclein, Pru p 7, following the FABER test (Supplemenatary Table 2). Four of them (1)(2)(3)(4) proved to be IgE positive to the pomegranate homologous protein, Pun g 7.…”