Proton Nuclear Magnetic Resonance--Based Metabonomics for Rapid Diagnosis of Meningitis and Ventriculitis

Coen, Muireann; O’Sullivan, Matthew; Bubb, William A.; Kuchel, Philip W.; Sorrell, Tania C.

doi:10.1086/497836

Cited by 105 publications

(81 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This technique has been applied to the study of organ-specific toxicity, 63 characterization of tissue composition and structure, 64 the analysis of needle biopsies of human samples, 65 cell systems, 55 tumors, 51,66 and to study dynamic information in tissue compartments. 19 Since only ϳ10 mg of tissue is needed, this approach is directly applicable to clinical biopsy samples. Like MRS, the exact quantitation of metabolite changes is difficult, as it is not possible to add an external quantitation standard.…”

Section: Key Analytical Developments In Nmr Spectroscopymentioning

confidence: 99%

“…Coen et al 19 Multiple sclerosis Characterization of the metabolic consequences during relapsingremitting episodes of MS Kapeller et al, 23 Karrenbauer et al, 24 Tartaglia and Arnold, 25 Matthews et al, 37 Tourbah et al, 34 Mader et al, 130 Arnold et al 131,135 Cushing's syndrome Brain metabolite alterations in Cushing's syndrome Khiat et al 31,32 Amyotrophic lateral sclerosis Screening the efficacy of potential therapeutic agents for ALS Kalra 36 …”

Section: Meningitis and Ventriculitismentioning

confidence: 99%

“…Initial pilot 1 H MRS studies have shown alterations in glutamate and glutamine ratios after creatine treatment that may be indicative of creatine enhancing the energy-dependent conversion of glutamate to glutamine, which has been shown to be perturbed in HD. 76,90,121 A recent study by Coen and coworkers 19 showed that, on the basis of NMR spectroscopic analysis of human CSF, viral meningitis could be differentiated from meningitis of bacterial or fungal origin. A range of metabolites, of both mammalian and microbial origin, were responsible for disease classification.…”

Section: The Use Of Metabonomics In Development Of Therapeuticsmentioning

confidence: 99%

See 2 more Smart Citations

The application of NMR-based metabonomics in neurological disorders

Holmes

Tsang

Tabrizi

2006

NeuroRX

View full text Add to dashboard Cite

Summary: Advances in postgenomic technologies have radically changed the information output from complex biological systems, generating vast amounts of high complexity data that can be interpreted by means of chemometric and bioinformatic methods to achieve disease diagnosis and prognosis. Highresolution nuclear magnetic resonance (NMR) spectroscopy of biofluids such as plasma, cerebrospinal fluid (CSF), and urine can generate robust, interpretable metabolic fingerprints that contain latent information relating to physiological or pathological status. This technology has been successfully applied to both preclinical and clinical studies of neurodegenerative diseases such as Huntington's disease, muscular dystrophy, and cerebellar ataxia. An extension of this technology, 1 H magicangle-spinning (HRMAS) NMR spectroscopy, can be used to generate metabolic information on small intact tissue samples, providing a metabolic link between metabolic profiling of biofluids and histology. In this review we provide a summary of high-resolution NMR studies in neurodegenerative disease and explore the potential of metabonomics in evaluating disease progression with respect to therapeutic intervention.

show abstract

Section: Key Analytical Developments In Nmr Spectroscopymentioning

confidence: 99%

Section: Meningitis and Ventriculitismentioning

confidence: 99%

Section: The Use Of Metabonomics In Development Of Therapeuticsmentioning

confidence: 99%

See 1 more Smart Citation

The application of NMR-based metabonomics in neurological disorders

Holmes

Tsang

Tabrizi

2006

NeuroRX

View full text Add to dashboard Cite

show abstract

“…Metabolic changes that reflect infection such as low glucose or disproportionately elevated lactate are characteristic of bacterial meningitis but have been poor discriminators in some studies [6][7][8]. A disadvantage of these biochemical methods is that only particular compounds are targeted by individual tests.…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Spectroscopy-Derived Tissue Oxygen Saturation in Battlefield Injuries: A Case Series Report

2016

Spectroscopy

View full text Add to dashboard Cite

Bacterial meningitis is an acute disease with high mortality that is reduced by early treatment. Identification of the causative microorganism by culture is sensitive but slow. Large volumes of cerebrospinal fluid (CSF) are required to maximise sensitivity and establish a provisional diagnosis.We have utilised nuclear magnetic resonance (NMR) spectroscopy to rapidly characterise the biochemical profile of CSF from normal rats and animals with pneumococcal or cryptococcal meningitis. Use of a miniaturised capillary NMR system overcame limitations caused by small CSF volumes and low metabolite concentrations. The analysis of the complex NMR spectroscopic data by a supervised statistical classification strategy included major, minor and unidentified metabolites.Reproducible spectral profiles were generated within less than three minutes, and revealed differences in the relative amounts of glucose, lactate, citrate, amino acid residues, acetate and polyols in the three groups. Contributions from microbial metabolism and inflammatory cells were evident. The computerised statistical classification strategy is based on both major metabolites and minor, partially unidentified metabolites. This data analysis proved highly specific for diagnosis (100% specificity in the final validation set), provided those with visible blood contamination were excluded from analysis; 6-8% of samples were classified as indeterminate.This proof of principle study suggests that a rapid etiologic diagnosis of meningitis is possible without prior culture. The method can be fully automated and avoids delays due to processing and selective identification of specific pathogens that are inherent in DNA-based techniques.

show abstract

“…[18][19][20][21] Due to noninvasive collection and easy acquisition of multiple samples in a short time, urine is considered as a common biofluid for metabolomic investigations. Urine gives a "time-averaged" pattern for polar metabolites that are excreted in variable amounts according to the variations in whole-body homeostatic control.…”

Section: Introductionmentioning

confidence: 99%

Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies

Liu¹,

Sun²,

Di³

et al. 2012

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at −20 o C for two months.

show abstract

Proton Nuclear Magnetic Resonance--Based Metabonomics for Rapid Diagnosis of Meningitis and Ventriculitis

Cited by 105 publications

References 31 publications

The application of NMR-based metabonomics in neurological disorders

The application of NMR-based metabonomics in neurological disorders

Near-Infrared Spectroscopy-Derived Tissue Oxygen Saturation in Battlefield Injuries: A Case Series Report

Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies

Contact Info

Product

Resources

About