volume 1, issue 2, P100089 2020
DOI: 10.1016/j.xpro.2020.100089
View full text
Sign up to set email alerts

Abstract: SUMMARY By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native conformation. BN-PAGE was first developed to analyze the size, composition, and relative abundance of the complexes and supercomplexes that form the mitochondrial respiratory chain and OXPHOS syste…

Expand abstract