Protocol for Evaluating the Biological Stability of Fuel Formulations and Their Relationship to Carbon Steel Biocorrosion

Anaerobic hydrocarbon biodegradation and biocorrosion of carbon steel in marine environments: The impact of different ultra low sulfur diesels and bioaugmentation

“…Environmental samples were cooled, shipped 140 to the laboratory, and stored refrigerated until use. Anaerobic incubations were prepared 141 in 160-ml serum bottles according to the protocol of Liang and Suflita (2015). The 142…”

“…The restricted substrate range exhibited by 148 the inoculum has so far been corroborated by a preliminary annotation of the organism's 149 genome (data not shown). The coupons were suspended in the seawater via teflon threads 150 affixed to the serum bottle closures (Liang and Suflita, 2015). The headspace was 151 adjusted to contain 80:20 N 2 :CO 2 .…”

“…Benzoate (10 mM) and decane (0.02 ml) were used as positive control 160 substrates. Microbial metabolism was routinely monitored by ion chromatography (Liang 161 and Suflita, 2015) as the loss of sulfate relative to negative controls. 162…”

A semi-continuous system for monitoring microbially influenced corrosion

“…The Food and Drug Administration (FDA) approved TW80 as a solubilizing and dispersing agent in pickles Figure 1. A) Diagram of a serum bottle incubation (Liang et al, 2015) B) Schematic of a once-through flow cell (Harris et al, 2010) and vitamins, and as an emulsifier in ice cream and sherbet. TW80 has also been documented to reduce end-product inhibition of Rhodococcus erythropolis during desulfurization (Feng et al, 2006).…”

“…Two types of systems, dynamic and static, are commonly used for studying MIC in a laboratory setting (Harris et al, 2010;Liang et al, 2015; Figure 1). An example of a dynamic system is the once-through flow cell (Duncan et al, 2014;Harris et al, 2010;Stipanicev et al, 2013;Tanner, 1985).…”