volume 1, issue 3, P100166 2020
DOI: 10.1016/j.xpro.2020.100166
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Abstract: Summary This protocol describes BATTLE-1EX, which is a combined method of BATTLE-1 and expansion microscopy to obtain high-resolution imaging of whole synaptic structures and their components of hippocampal neural circuits. BATTLE-1 uses two genetically engineered recombinase proteins and competition between two recombinases that can be independently titrated, resulting in a tunable proportion of mCherry+/YFP− and YFP+/mCherry− cells. As a combinational method, BATTLE-1EX has the potential to visual…

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