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“…Through recent efforts, significant progress has been made to begin to characterize the cellulase system in this species. Several protozoan species are known to play a central role in the R. flavipes digestive system, contributing endoglucanase and exoglucanase cellulase enzymes (Lewis and Forschler, 2004;Zhou et al, 2007). In this study, UV irradiation was used as a defaunation method to remove protozoan symbionts from R. flavipes hindguts.…”
Section: Discussionmentioning
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“…Through recent efforts, significant progress has been made to begin to characterize the cellulase system in this species. Several protozoan species are known to play a central role in the R. flavipes digestive system, contributing endoglucanase and exoglucanase cellulase enzymes (Lewis and Forschler, 2004;Zhou et al, 2007). In this study, UV irradiation was used as a defaunation method to remove protozoan symbionts from R. flavipes hindguts.…”
Section: Discussionmentioning
“…Dissections were carried out by immobilizing each termite on ice, gently holding the head and then extracting the hindgut by pulling on the last abdominal segments. The protocol used for protozoan counts was described by Lewis and Forschler (2004) with minor modifications. Protozoan counts were conducted by placing each hindgut into a microcentrifuge tube containing 100 ml of ice-cold 1 Â phosphate buffered saline (PBS).…”
Section: Protozoan Countsmentioning
“…flavipes from sequence of the 16S mitochondrial-ribosomal RNA gene, (Szalanski et al, 2003), gut fauna (Lewis and Forschler, 2004) and soldier morphology (Nutting, 1990).…”
Section: Methodsmentioning
“…Of the 11 species reported from the neighboring state of Georgia (Lewis and Forschler, 2004), we identified 10 protozoan species in our samples. The total protozoan population estimates and the relative protozoan species abundance were within the ranges reported previously (Cleveland, 1925;Lewis and Forschler, 2004). The one we did not find is Microfoenia fallax (Dubosq and Grassé).…”
Section: Body Mass and Vitalitymentioning
“…The hindgut protozoa were prepared using the method developed by Lewis and Forschler (2004) and were identified using the nondichotomous key (Lewis and Forschler, 2006). In brief, the hindgut of a termite was gently removed by pulling off the last two segments of the abdomen with a pair of forceps.…”
Section: Hindgut Protozoa Profilementioning