Proteomics of vitreous in neovascular age-related macular degeneration

Nobl, Matthias; Reich, Michael; Dacheva, Ivanka; Siwy, Justyna; Mullen, William; Schanstra, Joost P.; Choi, Chul Young; Kopitz, Jürgen; Kretz, Florian; Auffarth, Gerd U.; Koch, Frank; Koss, Michael

doi:10.1016/j.exer.2016.01.001

Cited by 43 publications

(50 citation statements)

References 47 publications

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“…Glutathione peroxidase 3 was also elevated in vitreous samples from patients with neovascular AMD, which was suggested to indicate oxidative stress in neovascular AMD. Nobl et al [98] analyzed 108 vitreous samples from patients suffering from neovascular AMD, which were compared to 24 vitreous samples from patients with idiopathic floaters using capillary electrophoresis coupled to MS. In vitreous samples from patients with neovascular AMD, the researchers identified an upregulation of clusterin, PEDF and prostaglandin H2-D isomerase, while opticin was found to be downregulated in neovascular AMD.…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

“…Thus, there is solid evidence that these proteins are changed in content in neovascular AMD. PEDF has been found to be upregulated in aqueous humor [96] and in vitreous samples [98] from patients with neovascular AMD. In CNV, the upregulation of PEDF is thought to be driven by VEGF [98].…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

“…PEDF has been found to be upregulated in aqueous humor [96] and in vitreous samples [98] from patients with neovascular AMD. In CNV, the upregulation of PEDF is thought to be driven by VEGF [98]. Clusterin has been found to be upregulated in vitreus samples [98] and in the choroid/Bruch membrane complex [92] in patients with neovascular AMD.…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

“…In CNV, the upregulation of PEDF is thought to be driven by VEGF [98]. Clusterin has been found to be upregulated in vitreus samples [98] and in the choroid/Bruch membrane complex [92] in patients with neovascular AMD. Furthermore, proteomic analyses of aqueous humor have found that clusterin was only detectable in aqueous humor from patients with neovascular AMD and not in controls undergoing cataract surgery [96].…”

Section: Age-related Macular Degenerationmentioning

confidence: 99%

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A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

Cehofski

Honoré

Vorum

2017

IJMS

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Retinal artery occlusion (RAO), retinal vein occlusion (RVO), diabetic retinopathy (DR) and age-related macular degeneration (AMD) are frequent ocular diseases with potentially sight-threatening outcomes. In the present review we discuss major findings of proteomic studies of RAO, RVO, DR and AMD, including an overview of ocular proteome changes associated with anti-vascular endothelial growth factor (VEGF) treatments. Despite the severe outcomes of RAO, the proteome of the disease remains largely unstudied. There is also limited knowledge about the proteome of RVO, but proteomic studies suggest that RVO is associated with remodeling of the extracellular matrix and adhesion processes. Proteomic studies of DR have resulted in the identification of potential therapeutic targets such as carbonic anhydrase-I. Proliferative diabetic retinopathy is the most intensively studied stage of DR. Proteomic studies have established VEGF, pigment epithelium-derived factor (PEDF) and complement components as key factors associated with AMD. The aim of this review is to highlight the major milestones in proteomics in RAO, RVO, DR and AMD. Through large-scale protein analyses, proteomics is bringing new important insights into these complex pathological conditions.

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Section: Age-related Macular Degenerationmentioning

confidence: 99%

Section: Age-related Macular Degenerationmentioning

confidence: 99%

Section: Age-related Macular Degenerationmentioning

confidence: 99%

Section: Age-related Macular Degenerationmentioning

confidence: 99%

See 2 more Smart Citations

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

Cehofski

Honoré

Vorum

2017

IJMS

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“…Correspondingly, SERPINF1 has been tested as a potential anti-angiogenic agent, especially in the eye, whereby its protective effects against neovascularization are essential in the AH to maintain the transparency of the cornea and lens [52, 59–61]. Conversely, increased levels of this protein were demonstrated in the aqueous and vitreous humour of AMD patients [17, 62]. Although no gender-based alterations of this protein have been reported in the eye, SERPINF1 level was elevated in serum samples of women with polycystic ovary syndrome associated with insulin resistance and reduced in peritoneal fluid of women with endometriosis [63, 64].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the human aqueous humour proteome: A comparison of the genders

et al. 2017

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Aqueous humour (AH) is an important biologic fluid that maintains normal intraocular pressure and contains proteins that regulate the homeostasis of ocular tissues. Any alterations in the protein compositions are correlated to the pathogenesis of various ocular disorders. In recent years, gender-based medicine has emerged as an important research focus considering the prevalence of certain diseases, which are higher in a particular sex. Nevertheless, the inter-gender variations in the AH proteome are unknown. Therefore, this study endeavoured to characterize the AH proteome to assess the differences between genders. Thirty AH samples of patients who underwent cataract surgery were categorized according to their gender. Label-free quantitative discovery mass spectrometry-based proteomics strategy was employed to characterize the AH proteome. A total of 147 proteins were identified with a false discovery rate of less than 1% and only the top 10 major AH proteins make up almost 90% of the total identified proteins. A large number of proteins identified were correlated to defence, immune and inflammatory mechanisms, and response to wounding. Four proteins were found to be differentially abundant between the genders, comprising SERPINF1, SERPINA3, SERPING1 and PTGDS. The findings emerging from our study provide the first insight into the gender-based proteome differences in the AH and also highlight the importance in considering potential sex-dependent changes in the proteome of ocular pathologies in future studies employing the AH.

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Mass spectrometry‐based retina proteomics

Prókai

Zaman

Prókai-Tátrai

2022

Mass Spectrometry Reviews

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A subfield of neuroproteomics, retina proteomics has experienced a transformative growth since its inception due to methodological advances in enabling chemical, biochemical, and molecular biology techniques. This review focuses on mass spectrometry's contributions to facilitate mammalian and avian retina proteomics to catalog and quantify retinal protein expressions, determine their posttranslational modifications, as well as its applications to study the proteome of the retina in the context of biology, health and diseases, and therapy developments.

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Proteomics of vitreous in neovascular age-related macular degeneration

Cited by 43 publications

References 47 publications

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders

Characterization of the human aqueous humour proteome: A comparison of the genders

Mass spectrometry‐based retina proteomics

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