Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse

Li, Xiang‐Juan; Pan, Haitian; Chen, Juanjuan; Fu, Yi‐Bin; Fang, Min; He, Guohua; Zhang, Tao; Ding, Haigang; Cheng, Yi; Tan, Ya‐Jing; Zhao, Fa‐Lin; Morse, Abraham N.; Huang, He‐Feng

doi:10.1002/prca.201800086

Cited by 4 publications

(2 citation statements)

References 25 publications

(20 reference statements)

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“…After that, the PD software was used to perform a quantitative analysis based on the search results and selected mass spectra. In the proteomic analysis, a fold change >1.2 was regarded to indicate a DEP between two groups according to previous studies ( 18 – 20 ). According to the outcome of the quantitative analysis, all identified DEPs were subjected to functional annotation using the GO ( ) and KEGG databases ( ).…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of radioiodine‑refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium‑iodine symporter

Deng

et al. 2022

Oncol Lett

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Radioiodine refractory differentiated thyroid cancer (RR-DTC) is the main factor adversely affecting the overall survival rate of patients with thyroid cancer. The aim of the present study was to investigate the underlying molecular mechanism of pathogenesis of RR-DTC and to explore novel therapeutic targets for clinical treatment. A proteomic analysis was performed using the tumor tissues of patients with RR-DTC. A total of 6 metastatic lymph nodes were collected during lymph node dissection, 3 from patients with RR-DTC and 3 from patients with papillary thyroid cancer. The expression of chitinase-3-like 1 (CHI3L1) and sodium-iodine symporter (NIS) in the tumor tissue was detected by immunohistochemistry (IHC). Western blotting was used to detect the expression of CHI3L1, phosphorylated (p)-MEK and p-ERK1/2 in PTC-K1 cells transfected with CHI3L1 overexpression vector. The proteomic analysis identified 665 differentially expressed proteins (DEPs), including 327 upregulated and 338 downregulated proteins in the RR-DTC group, which were enriched in 59 signaling pathways by Kyoto Encyclopedia of Genes and Genomes database analysis. In particular, CHI3L1 was demonstrated to be significantly upregulated in RR-DTC as evidenced by quantitative proteomic analysis and IHC. Western blotting suggested that the overexpression of CHI3L1 activated the MEK/ERK1/2 signaling pathway, which may lead to NIS dysfunction. In conclusion, the present study suggests that CHI3L1 is a potential molecular target for the radiotherapy of patients with RR-DTC.

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Section: Methodsmentioning

confidence: 99%

Proteomic analysis of radioiodine‑refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium‑iodine symporter

Deng

et al. 2022

Oncol Lett

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“…These proteins may be involved in the pathophysiology of POP. Further proteomic analysis of the etiology and pathogenesis of POP using HPLC-MS/MS, iTRAQ, and ingenuity pathway analysis (IPA) techniques described by Li et al ( 98 ), revealed five differentially expressed proteins (fibromodulin, collagen alpha-1 [XIV] chain, calponin-1, tenascin, and galectin-1) that appear to be involved in Metabolic mechanisms of the pelvic floor connective tissue. Wang et al ( 99 ).…”

Section: Other Potential Biomarkersmentioning

confidence: 99%

Potential molecular targets for intervention in pelvic organ prolapse

Wu,

Liu,

2023

Front. Med.

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Pelvic organ prolapse (POP) is a concerning gynecological benign illness in middle-aged and senior women. Its etiology is complex, the incidence rate is high, symptoms are clinically subjective, and its influence tends to be polarized. At present, for those who need medical treatment, whether surgical or non-surgical, complications cannot be ignored, and treatment effect needs to be optimized. However, there is a lack of accurate molecular biological interventions for the prevention, diagnosis, progression delay, and treatment of POP. Here, we reviewed the current state of understanding of the molecular mechanisms and factors associated with POP etiology. These factors include cyclins, matrix metal peptidases/tissue inhibitors of metalloproteinases, microRNAs, homeobox A11, transforming growth factor β1, insulin-like growth factor 1, fibulin 5, lysyl oxidase-like 1, oxidative stress, inflammatory response, estrogen, and other potential biomarkers associated with POP. In addition, relevant molecular targets that may be used to intervene in POP are summarized. The aim of this review was to provide more information to identify accurate potential biomarkers and/or molecular targets for the prevention, diagnosis, progression delay, and treatment of POP, with the goal of improving medical treatment for patients at-risk for POP or having POP. Continued research is needed to identify additional details of currently accepted molecular mechanisms and to identify additional mechanisms that contribute to POP.

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Exosomes Derived from miR‐195‐5p Transfected Bone Marrow Mesenchymal Stem Cells Alleviate Pelvic Floor Dysfunction in Rats

Chen

Wan

Zhou

et al. 2022

Advanced Therapeutics

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Bone marrow-derived mesenchymal stem cells (BMSCs) can alleviate pelvic floor dysfunction (PFD) in rats. Whether exosomes derived from miR-195-5p transfected BMSCs can alleviate PFD is investigated. The rat vaginal distention model is constructed to mimic PFD. miR-195-5p is transfected into BMSCs, and exosomes derived from miR-195-5p transfected BMSCs are injected into PFD rats via caudal veins. Luciferase reporter assay is performed to confirm the direct interaction between SMAD7 3′-UTR and miR-195-5p mimics. Void volume, bladder void pressure, leak point pressure, and peak bladder pressure are used to evaluate urodynamic function. It is found that miR-195-5p directly targets Smad7 and regulates the relative Smad7 expression in rat BMSCs. Exosomes derived from miR-195-5p transfected BMSCs can improve urodynamic function than exosomes derived from BMSCs. On the other hand, exosomes derived from miR-195-5p transfected BMSCs inhibit the expressions of Smad7 but promote the expressions of elastin in PFD rats with diminished expression of MMP2 and MMP9. This study demonstrates that exosomes derived from miR-195-5p genetically engineered BMSCs can target the Tgf-𝜷/Smad7 pathway to repair connective tissues and restore damaged urodynamic function in PFD rats.

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Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse

Cited by 4 publications

References 25 publications

Proteomic analysis of radioiodine‑refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium‑iodine symporter

Proteomic analysis of radioiodine‑refractory differentiated thyroid cancer identifies CHI3L1 upregulation in association with dysfunction of the sodium‑iodine symporter

Potential molecular targets for intervention in pelvic organ prolapse

Exosomes Derived from miR‐195‐5p Transfected Bone Marrow Mesenchymal Stem Cells Alleviate Pelvic Floor Dysfunction in Rats

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