Proteomics of Long‐Lived Mammals

Tombline, Gregory; Gigas, Jonathan; Macoretta, Nicholas; Zacher, Max; Emmrich, Stephan; Zhao, Yang; Seluanov, Andrei; Gorbunova, Vera

doi:10.1002/pmic.201800416

Cited by 8 publications

(8 citation statements)

References 153 publications

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“…Ma and colleagues found that the proteomic and metabolomic signatures of individual cell lines appeared to be conserved among longevous species, including birds [80]. This is consistent with similar data from whole-animal models, including Ames dwarf mice [166], bats [167], and naked mole-rats [168]. The proteomic and metabolomic signatures were also consistent with those from studies employing life-extending interventions, such as caloric restriction [169].…”

Section: Avian Proteomics Lipidomic and Metabolomicssupporting

confidence: 58%

New Perspectives on Avian Models for Studies of Basic Aging Processes

Harper

Holmes

2021

Biomedicines

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Avian models have the potential to elucidate basic cellular and molecular mechanisms underlying the slow aging rates and exceptional longevity typical of this group of vertebrates. To date, most studies of avian aging have focused on relatively few of the phenomena now thought to be intrinsic to the aging process, but primarily on responses to oxidative stress and telomere dynamics. But a variety of whole-animal and cell-based approaches to avian aging and stress resistance have been developed—especially the use of primary cell lines and isolated erythrocytes—which permit other processes to be investigated. In this review, we highlight newer studies using these approaches. We also discuss recent research on age-related changes in neural function in birds in the context of sensory changes relevant to homing and navigation, as well as the maintenance of song. More recently, with the advent of “-omic” methodologies, including whole-genome studies, new approaches have gained momentum for investigating the mechanistic basis of aging in birds. Overall, current research suggests that birds exhibit an enhanced resistance to the detrimental effects of oxidative damage and maintain higher than expected levels of cellular function as they age. There is also evidence that genetic signatures associated with cellular defenses, as well as metabolic and immune function, are enhanced in birds but data are still lacking relative to that available from more conventional model organisms. We are optimistic that continued development of avian models in geroscience, especially under controlled laboratory conditions, will provide novel insights into the exceptional longevity of this animal taxon.

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Section: Avian Proteomics Lipidomic and Metabolomicssupporting

confidence: 58%

New Perspectives on Avian Models for Studies of Basic Aging Processes

Harper

Holmes

2021

Biomedicines

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“…For example, one of the most common malignancies following transplantation is Kaposi sarcoma which has a standardized incidence ratio (SIR) averaging 208, a significant increase when compared to 20 SIR in a pre-dialysis study population [ 36 ]. In recent years, the power of proteomics has shown how large scale data can inform on senescence associated phenotypes and ageing [ 30 , 37 , 38 ]. Here we applied the power of temporal proteomics to demonstrate that pathways enriched in the death proteome are the same pathways triggered in phenotypes of ageing and senescence which will have sobering consequences for all our well being.…”

Section: Discussionmentioning

confidence: 99%

The Molecular Floodgates of Stress-Induced Senescence Reveal Translation, Signalling and Protein Activity Central to the Post-Mortem Proteome

Wasinger

Curnoe

Boel

et al. 2020

IJMS

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The transitioning of cells during the systemic demise of an organism is poorly understood. Here, we present evidence that organismal death is accompanied by a common and sequential molecular flood of stress-induced events that propagate the senescence phenotype, and this phenotype is preserved in the proteome after death. We demonstrate activation of “death” pathways involvement in diseases of ageing, with biochemical mechanisms mapping onto neurological damage, embryonic development, the inflammatory response, cardiac disease and ultimately cancer with increased significance. There is sufficient bioavailability of the building blocks required to support the continued translation, energy, and functional catalytic activity of proteins. Significant abundance changes occur in 1258 proteins across 1 to 720 h post-mortem of the 12-week-old mouse mandible. Protein abundance increases concord with enzyme activity, while mitochondrial dysfunction is evident with metabolic reprogramming. This study reveals differences in protein abundances which are akin to states of stress-induced premature senescence (SIPS). The control of these pathways is significant for a large number of biological scenarios. Understanding how these pathways function during the process of cellular death holds promise in generating novel solutions capable of overcoming disease complications, maintaining organ transplant viability and could influence the findings of proteomics through “deep-time” of individuals with no historically recorded cause of death.

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“…Nowadays, individuals enjoy greater longevity, although this is not always accompanied by good health or quality of life. The mechanisms linked to aging identify nine key points of cellular alteration that mediate this process: increased senescence-associated secretory phenotype (SASP), stem cell depletion, altered cell communication, genomic instability, loss of telomere material, functional alteration in mitochondria, impaired nutrient perception, loss of proteostasis, and epigenetic alterations [ 73 ]. Proteomics allows the comprehensive cataloging of complete protein complements as a tool to identify protein alterations in aging.…”

Section: Role Of the Proteome In Agingmentioning

confidence: 99%

“…The study of proteomes identifies a potential link between post-translational modification (PTM) and metabolism in long-lived mammals [ 73 ]. For this purpose, the OMAscan test, which uses single-stranded nucleic acids or oligonucleotides, aptamers, present in proteomes, is a key tool to identify proteins related to various cellular mechanisms of aging [ 81 ].…”

Section: Potential Proteomic Biomarkers Involved In Agingmentioning

confidence: 99%

Potential Satellite Cell-Linked Biomarkers in Aging Skeletal Muscle Tissue: Proteomics and Proteogenomics to Monitor Sarcopenia

et al. 2022

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Sarcopenia (Sp) is the loss of skeletal muscle mass associated with aging which causes an involution of muscle function and strength. Satellite cells (Sc) are myogenic stem cells, which are activated by injury or stress, and repair muscle tissue. With advancing age, there is a decrease in the efficiency of the regenerative response of Sc. Diagnosis occurs with the Sp established by direct assessments of muscle. However, the detection of biomarkers in real-time biofluids by liquid biopsy could represent a step-change in the understanding of the molecular biology and heterogeneity of Sp. A total of 13 potential proteogenomic biomarkers of Sp by their physiological and biological interaction with Sc have been previously described in the literature. Increases in the expression of GDF11, PGC-1α, Sirt1, Pax7, Pax3, Myf5, MyoD, CD34, MyoG, and activation of Notch signaling stimulate Sc activity and proliferation, which could modulate and delay Sp progression. On the contrary, intensified expression of GDF8, p16INK4a, Mrf4, and activation of the Wnt pathway would contribute to early Sp development by directly inducing reduced and/or altered Sc function, which would attenuate the restorative capacity of skeletal muscle. Additionally, tissue biopsy remains an important diagnostic tool. Proteomic profiling of aged muscle tissues has shown shifts toward protein isoforms characteristic of a fast-to-slow transition process and an elevated number of oxidized proteins. In addition, a strong association between age and plasma values of growth differentiation factor 15 (GDF-15) has been described and serpin family A member 3 (serpin A3n) was more secreted by atrophied muscle cells. The identification of these new biomarkers holds the potential to change personalized medicine because it could predict in real time the course of Sp by monitoring its evolution and assessing responses to potential therapeutic strategies.

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Proteomics of Long‐Lived Mammals

Cited by 8 publications

References 153 publications

New Perspectives on Avian Models for Studies of Basic Aging Processes

New Perspectives on Avian Models for Studies of Basic Aging Processes

The Molecular Floodgates of Stress-Induced Senescence Reveal Translation, Signalling and Protein Activity Central to the Post-Mortem Proteome

Potential Satellite Cell-Linked Biomarkers in Aging Skeletal Muscle Tissue: Proteomics and Proteogenomics to Monitor Sarcopenia

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