Proteomics-based analysis of differentially expressed proteins in the CXCR1-knockdown gastric carcinoma MKN45 cell line and its parental cell

Abstract

C-X-C chemokine receptor types 1 (CXCR1), a cell-surface G-protein-coupled receptor has been found to be associated with tumorigenesis, development, and progression of some tumors. Previously, we have found that CXCR1 overexpression is associated with late-stage gastric adenocarcinoma. We also have demonstrated that knockdown of CXCR1 could inhibit cell proliferation in vitro and in vivo. In this study, we compared the changes of protein expression profile between gastric carcinoma MKN45 cell line and CXCR1-knockdown MKN45 cell line by 2D electrophoresis. Among the 101 quantified proteins, 29 spots were significantly different, among which 13 were downregulated and 16 were up-regulated after CXCR1 knockdown. These proteins were further identified by mass spectrometry analysis. Among them, several up-regulated proteins such as hCG2020155, Keratin8, heterogeneous nuclear ribonucleoprotein C (C1/C2), and several downregulated proteins such as Sorcin, heat shock protein 27, serpin B6 isoform b, and heterogeneous nuclear ribonucleoprotein K were confirmed. These proteins are related to cell cycle, the transcription regulation, cell adherence, cellular metabolism, drug resistance, and so on. These results provide an additional support to the hypothesis that CXCR1 might play an important role in proliferation, invasion, metastasis, and prognosis, and drug resistance of gastric carcinoma.

Expression of CPEB4 in Human Glioma and Its Correlations With Prognosis

“…Western blotting was performed in accordance with a previously described protocol. 12 Cells were collected from flasks and washed 3 times with cold PBS. Tissue samples (3 glioma tissues and 1 normal brain tissue resected for the treatment of nonglioma disease) were ground with liquid nitrogen and lysed at 4°C for 30 minutes in lysis buffer (50 mM Tris, pH 7.4, 100 mM NaCl 2 , 1 mM MgCl 2 , 2.5 mM Na 3 VO 4, 1 mM phenylmethylsulfonyl fluoride, 2.5 mM EDTA, 0.5% Triton X-100, 0.5% NP-40, and 5 mg/mL each of aprotinin, pepstatin A, and leupeptin).…”

The Rest of Interleukins

“…The two receptors share a 78% homology (Murphy, 1994). However, differences in their N-terminal domains result in different binding specificities (Murphy, 1994;Hu et al, 2013).…”

CXC chemokine receptor 1 predicts postoperative prognosis and chemotherapeutic benefits for TNM II and III resectable gastric cancer patients

“…The knockdown of CXCR1 was reported to be able to prohibit the proliferation of cancer cells and even induce tumor cell apoptosis in gastric cancer. Also, the knockdown of CXCR1 could lead to the down-regulation of the phosphorylation level of serine/threonine protein kinase (AKT) and extracellular signal-regulated kinase (ERK) 1/2 [33], indicating that AKT and ERK might be involved in the downstream of CXCR1 signal pathway. Another report showed that CXCR1 could up-regulate matrix metalloproteinase-9 (MMP-9) expression by activating JNK/c-Jun and ERK/Ets-1 pathways [34, 35], thus resulting in more aggressive biological characteristics of cancer cells.…”