Proteomics Analysis of Plant Tissues Based on Two-Dimensional Gel Electrophoresis

The Use of Proteomics in Search of Allele-Specific Proteins in (Allo)polyploid Crops

“…Examples are numerous in the literature and it is out of scope to make a general review on the subject. Essential application examples are focused on the analysis of plant proteomes [51], the discovery of specific proteins [52] the detection of expression differences upon specific conditions [53] and the discovery of plant allergens [54]. Overall, various plant organs extracts have been thus analyzed with the intervention of combinatorial peptide ligand libraries and few illustrative examples are given.…”

Screening acacia mearnsii (black wattle) seedlings for frost tolerance using an artificial frost technique, combined with proteomic analysis.

“…This is especially important for quantitative traits which involve the accumulative action of many genes (Thelen and Peck, 2007). Improvements in plant protein extraction methods and resolution techniques such as two-dimensional gel electrophoresis has enabled the analysis of protein expression profiles (Jorrin-Novo et al, 2018). This is useful as protein expression changes in relation to stresses such as cold conditions.…”