Proteomic Profile ofMabuyasp. (Squamata: Scincidae) Ovary and Placenta During Gestation

Abstract

Reptiles are one of the most diverse groups of vertebrates, providing an integrated system for comparative studies on metabolic, animal physiology, and developmental biology. However, the molecular data available are limited and only recently have started to call attention in the "omics" sciences. Mabuya sp. is a viviparous placentrotrophic skink with particular reproductive features, including microlecithal eggs, early luteolysis, prolonged gestation, and development of a highly specialized placenta. This placenta is responsible for respiratory exchange and the transference of all nutrients necessary for embryonic development. Our aim was to identify differentially expressed proteins in the ovary and placenta of Mabuya sp. during early, mid, and late gestation; their possible metabolic pathways; and biological processes. We carried out a comparative proteomic analysis during gestation in both tissues by sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis, and matrix-assisted laser desorption/ionization. Differential protein expression in both tissues (Student's t-test P < 0.05) was related to several processes such as cell structure, cell movement, and energy. Proteins found in ovary are mainly associated with follicular development and its regulation. In the placenta, particularly during mid and late gestation, protein expression is involved in nutrient metabolism, transport, protein synthesis, and embryonic development. This work provides new insights about the proteins expressed and their physiological mechanisms in Mabuya sp. placenta and ovary during gestation.

A Review: Comparative Genomics and Physiology of Parity Mode Evolution in Amniotes

“…Finally, the proteomes of the ovary and placenta from obligately placentrophic Mabuya lizards can further serve as a useful resource for examining nutrient provisioning in squamates (Hernández-Díaz et al, 2017). In the placenta they found protein expression involved with nutrient metabolism, transport, protein synthesis, and embryonic development (Hernández-Díaz et al, 2017).…”