Proteomic pattern of implantative human endometrial fluid in in vitro fertilization cycles

Matorras, Roberto; Quevedo, Sara; Corral, Blanca; Prieto, Begoña; Expósito, Antonia; Mendoza, Rosario; Rabanal, Aintzane; Díaz-Núñez, María; Ferrando, Marcos; Elortza, Félix; Ametzazurra, Amagoia; Nagore, Daniel

doi:10.1007/s00404-018-4753-1

Cited by 25 publications

(30 citation statements)

References 29 publications

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“…Histological evaluations have been regarded as the standard for the clinical diagnosis of endometrial abnormalities [14]. Recently, many studies have investigated the potential of proteomic analysis to characterize the expression patterns of physiologically active substances on proliferative and secretory endometrium [15][16][17]. The identi cation of gene expression pro les has led to a differential analysis of receptive and nonreceptive patterns in endometrial signaling.…”

Section: Discussionmentioning

confidence: 99%

The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters

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Kawano

Utsunomiya³

et al. 2021

Reprod. Sci.

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Background: Endometrial receptivity issues represent a potential source of implantaion failure. It is very important to investigate the expression of endometrial receptive markers in the endometrium during implantation. Therefore, we examined whether it would be possible to analyze endometrial receptivity using cells from embryo transfer catheters. In addition, we analyzed the relationship between the gene expression pro le associated with pregnancy from endometrial cells taken during embryo transfer.Methods: A total of 88 cycles from 88 consenting patients were enrolled in this study. The tip of the embryo transfer (ET) catheter was cut and immersed in a dedicated reagent. Con rmation of cell distribution was carried out using a Papanicolaou stain (n=6) and immunocytochemistry (n=3). Protein expression was carried out by immunocytochemistry (n=12). Total RNA was extracted, and the expression of endometrial receptive markers (estrogen receptor α, progesterone receptor, and homeobox A10) were analyzed using quantitative reverse transcription polymerase chain reaction (n=67). we analyzed the relationship between the gene expression pro le associated with pregnancy from endometrial cells.Results: Samples collected from the ET catheter showed clear staining for endometrial cells. Most of the cells were endometrial epithelial cells. Cervical cells were not included. The protein expression of endometrial receptive markers in cells was also con rmed. Three genes were analyzed that are associated with endometrial receptivity. Progesterone receptor expression was 1.5-fold and homeobox A10 expression was 2-fold higher in patients who became non-pregnant group, compared to the pregnant group. Both increases were statistically signi cant (p < 0.05). Estrogen receptor α expression tended to be higher in the non-pregnant group, but there was no signi cant difference. Conclusion:Our results suggest that endometrial receptivity can be evaluated using cells obtained from the ET catheter. This method may be useful for elucidating the cause of implantation failure by comparing a receptive and non-receptive endometrium at the time of ET.

show abstract

Section: Discussionmentioning

confidence: 99%

The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters

Goto

Kawano

Utsunomiya³

et al. 2021

Reprod. Sci.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

The Possibility of Analyzing Endometrial Receptivity Using Cells From Embryo Transfer Catheters

Goto

Kawano

Utsunomiya

et al. 2020

Preprint

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Background: Endometrial receptivity issues represent a potential source of implantaion failure. It is very important to investigate the expression of endometrial receptive markers in the endometrium during implantation. Therefore, we examined whether it would be possible to analyze endometrial receptivity using cells from embryo transfer catheters. In addition, we analyzed the relationship between the gene expression profile associated with pregnancy from endometrial cells taken during embryo transfer.Methods: A total of 88 cycles from 88 consenting patients were enrolled in this study. The tip of the embryo transfer (ET) catheter was cut and immersed in a dedicated reagent. Confirmation of cell distribution was carried out using a Papanicolaou stain (n=6) and immunocytochemistry (n=3). Protein expression was carried out by immunocytochemistry (n=12). Total RNA was extracted, and the expression of endometrial receptive markers (estrogen receptor α, progesterone receptor, and homeobox A10) were analyzed using quantitative reverse transcription polymerase chain reaction (n=67). we analyzed the relationship between the gene expression profile associated with pregnancy from endometrial cells. Results: Samples collected from the ET catheter showed clear staining for endometrial cells. Most of the cells were endometrial epithelial cells. Cervical cells were not included. The protein expression of endometrial receptive markers in cells was also confirmed. Three genes were analyzed that are associated with endometrial receptivity. Progesterone receptor expression was 1.5-fold and homeobox A10 expression was 2-fold higher in patients who became non-pregnant group, compared to the pregnant group. Both increases were statistically significant (p < 0.05). Estrogen receptor α expression tended to be higher in the non-pregnant group, but there was no significant difference. Conclusion: Our results suggest that endometrial receptivity can be evaluated using cells obtained from the ET catheter. This method may be useful for elucidating the cause of implantation failure by comparing a receptive and non-receptive endometrium at the time of ET.

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“…This protein plays a role in regulating cell morphology and cytoskeletal organisation [104]. CAPZB was founded to be down-regulated in the conception cycle compared to the non-conception cycle in the human endometrial fluid aspirate [105]. In livestock, functional analysis of proteomic profiles during the early stage of pregnancy was reported in cows [106], sows [25] as well as in sheep [107].…”

Section: Molecular Characterisation Of Down-regulated Deps During 9d 12d and 16d Of Pregnancymentioning

confidence: 99%

Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

Pierzchała

Liput

Korwin-Kossakowska

et al. 2021

IJMS

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The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGOTM approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts.

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Proteomic pattern of implantative human endometrial fluid in in vitro fertilization cycles

Abstract: We found a very different protein profile in implantative cycles, the majority of proteins being down-regulated. This probably reflects a different endometrial functional status, more favourable to implantation. EFA proteomic analysis could be a useful tool in the planning ET strategies.

Cited by 25 publications

References 29 publications

The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters

The Possibility of Analyzing Endometrial Receptivity Using Cells from Embryo Transfer Catheters

The Possibility of Analyzing Endometrial Receptivity Using Cells From Embryo Transfer Catheters

Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

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