Proteomic investigation of whole saliva in Wilson's disease

Cabras, Tiziana; Sanna, Maria Teresa; Manconi, Barbara; Fanni, Daniela; Demelia, Luigi; Sorbello, Orazio; Iavarone, Federica; Castagnola, Massimo; Faa, Gavino; Messana, Irene

doi:10.1016/j.jprot.2015.07.033

Cited by 26 publications

(35 citation statements)

References 60 publications

(72 reference statements)

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“…Oxidation occurred on methionine and tryptophan residues, and on the unique cysteine residue, in position 42 in S100A8, and 3 in S100A9, that generated glutathionylated, cysteinylated, sulphinic, sulphonic, and disulphide dimeric forms. These findings showed that the salivary proteome of Wilson's disease patients reflected oxidative stress and inflammatory conditions characteristic of the pathology 46 .…”

Section: Human Saliva As Diagnostic Body Fluidmentioning

confidence: 79%

Salivary biomarkers and proteomics: future diagnostic and clinical utilities

Castagnola

Scarano

Passàli

et al. 2017

Acta Otorhinolaryngol Ital

130

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Lo studio della proteomica salivare, test economico e non invasivo, rappresenta una fonte di numerose informazioni, ed è utile per la diagnosi di svariate malattie. Da quando siamo entrati nellera della tecnologia genomica e delle scienze omiche, la raccolta di campioni salivari è aumentata esponenzialmente. Recenti piattaforme proteomiche hanno analizzato il proteoma salivare umano, caratterizzando circa 3000 peptidi e proteine, espressi in maniera differente: più del 90% in peso deriva dalla secrezione delle tre ghiandole salivari maggiori, mentre la restante parte proviene dalle ghiandole salivari minori, dal fluido crevicolare gengivale, da essudati mucosi e dalla microflora orale. Lobiettivo principale dellanalisi proteomica è discriminare tra condizioni fisiologiche e patologiche. Ad oggi, tuttavia, non esiste un preciso protocollo che permetta di analizzare lintero proteoma salivare, pertanto sono state realizzate svariate strategie. Innanzitutto, è possibile distinguere due tipologie di piattaforme proteomiche: lapproccio top-down prevede lanalisi delle proteine sotto esame come entità intatte; nellapproccio bottom-up la caratterizzazione della proteina avviene mediante lo studio dei peptidi ottenuti dopo digestione enzimatica (con tripsina tipicamente). A causa di questa eterogeneità, per una stessa patologia sono stati proposti differenti biomarkers. Il proteoma salivare è stato caratterizzato in numerose malattie: carcinoma squamoso e leucoplachie orali, malattia del trapianto contro lospite (GVHD) cronica, sindrome di Sjögren e altri disordini autoimmuni come la sindrome SAPHO (sinovite, acne, pustolosi, iperostosi e osteite), schizofrenia e disordine bipolare, malattie genetiche come la sindrome di Down o la malattia di Wilson. In conclusione, i risultati delle ricerche riportate in questa review suggeriscono che nel prossimo futuro la saliva diverrà un fluido di indubbia rilevanza diagnostica utile per fini clinici, sia diagnostici, sia prognostici.

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Section: Human Saliva As Diagnostic Body Fluidmentioning

confidence: 79%

Salivary biomarkers and proteomics: future diagnostic and clinical utilities

Castagnola

Scarano

Passàli

et al. 2017

Acta Otorhinolaryngol Ital

130

View full text Add to dashboard Cite

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“…Overall, the oxidative stress and inflammatory conditions reflected by the salivary proteome of patients with Wilson's disease might be key clues of disease exacerbation. 55 Moreover, the level of salivary cortisol is a regular biomarker for psychology stress. Three potential biomarkers from saliva have been demonstrated to diagnose atopic dermatitis in its early stage.…”

Section: Diagnosis Of Systematic Diseases By Salivamentioning

confidence: 99%

Saliva in the diagnosis of diseases

et al. 2016

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Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects and digestion. With the rapid advancement in salivaomics, saliva is well recognized as a pool of biological markers. Saliva, as a non-invasive and safe source, could be a substitute for blood in the diagnosis and prognosis of diseases. This review summarizes the latest advancements in saliva-related studies and addresses the potential value of saliva in the early diagnosis of oral diseases, such as dental caries and periodontal disease, as well as cancer, diabetes and other systemic disorders. Saliva biomarkers range from changes in the biochemical indices of DNA, RNA and proteins to the diversification of microbiota structures. This study integrates data reported in the recent literature and discusses the clinical significance and prospects for the application of saliva in the early diagnosis of diseases, translational medicine and precision medicine.

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“…Thus, at least one PTM was expected to be localized in one of these peptides. S100A8 oxidation on methionine 1 and 78 has been reported in saliva of Wilson's disease patients [22], but this reversible oxidation, not maintained under the reducing conditions used in the 2-DE analysis [24], might not be responsible for the presence of two S100A8 spots. Spot 9 resulted 1.5 fold overexpressed in preterm newborn saliva with respect to at-term newborns and adults, while spot 8, corresponding to the S100A8 protein species with higher pI, did not show statistically significant differences.…”

Section: S100 Familymentioning

confidence: 96%

“…Adults showed a statistically significant increase of several proteins with respect to preterm and at-term newborns: E-FABP (3), S100A6 (57), S100A7 (44), two protein species of S100A9 (1B and 11), several protein species of PIP (15, 16 and 17), Ig kappa chain (22,45) two protein species of cystatin SN (27,34) and cystatin S/SA (19) and several protein species of α-amylase-1 (39,51,53,54,56). In 2-DE gel of preterm newborn saliva, only two protein species of albumin (64, 65) resulted increased with statistical significance (5.1 and 4.4 fold) with respect to at-term newborns and adults.…”

Section: Identification Of Differentially Expressed Proteins In Pretementioning

confidence: 99%

“…Uniprot database only reports Snitrosylation of Cys 42 as possible PTM for S100A8 [19]. In vitro oxidation of Cys 42 of S100A8 to sulfenic/sulfinic/sulfonic acid has been reported by several authors [20][21], while modification of Cys 42 to sulfinic and sulfonic acid, as well as nitrosylation and glutathionylation has been observed in the acid-soluble fraction of adult saliva [22]. Manual inspection of MS/MS spectra of tryptic peptides from spots 8 and 9 allowed to exclude the irreversible oxidation of Cys 42 residue to sulfinic/sulfonic acid and the nitrosylation because, after DTT reduction and IAM alkylation, Cys 42 was identified in the carbamidomethylated form while SNO modifications require different reducing conditions [23].…”

Section: S100 Familymentioning

confidence: 99%

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