Proteomic identification and profiling of canine lymphoma patients

Ratcliffe, Lucy; Mian, Shahid; Slater, K.; King, H.; Napolitano, Mariasanta; AuCoin, David P.; Mobasheri, Ali

doi:10.1111/j.1476-5829.2009.00165.x

Cited by 32 publications

(26 citation statements)

References 21 publications

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“…Proteomics has been applied in studies on cows [1-4], horses [5], swine [6], chickens [7], and some domestic pets [8], to define the protein profiles of tissues and body fluids during stressful conditions, pathological states, and infections.…”

Section: Introductionmentioning

confidence: 99%

Proteomic evaluation of sheep serum proteins

et al. 2012

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BackgroundThe applications of proteomic strategies to ovine medicine remain limited. The definition of serum proteome may be a good tool to identify useful protein biomarkers for recognising sub-clinical conditions and overt disease in sheep. Findings from bovine species are often directly translated for use in ovine medicine. In order to characterize normal protein patterns and improve knowledge of molecular species-specific characteristics, we generated a two-dimensional reference map of sheep serum. The possible application of this approach was tested by analysing serum protein patterns in ewes with mild broncho-pulmonary disease, which is very common in sheep and in the peripartum period which is a stressful time, with a high incidence of infectious and parasitic diseases.ResultsThis study generated the first reference 2-DE maps of sheep serum. Overall, 250 protein spots were analyzed, and 138 identified.Compared with healthy sheep, serum protein profiles of animals with rhino-tracheo-bronchitis showed a significant decrease in protein spots identified as transthyretin, apolipoprotein A1 and a significant increase in spots identified as haptoglobin, endopin 1b and alpha1B glycoprotein.In the peripartum period, haptoglobin, alpha-1-acid glycoprotein, apolipoprotein A1 levels rose, while transthyretin content dropped.ConclusionsThis study describes applications of proteomics in putative biomarker discovery for early diagnosis as well as for monitoring the physiological and metabolic situations critical for ovine welfare.

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Section: Introductionmentioning

confidence: 99%

Proteomic evaluation of sheep serum proteins

et al. 2012

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“…As opposed to tests which either non-specifically indicate a cancer by measuring thymidine kinase activity [45] or rely on biomarkers [46], the immunosignature is information rich. From a single assay, the patient could potentially be diagnosed as having LSA or not, whether the LSA is of B or T cell lineage and whether the DFI following chemotherapy will be short or long.…”

Section: Discussionmentioning

confidence: 99%

The immunosignature of canine lymphoma: characterization and diagnostic application

2014

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BackgroundCancer diagnosis in both dogs and humans is complicated by the lack of a non-invasive diagnostic test. To meet this clinical need, we apply the recently developed immunosignature assay to spontaneous canine lymphoma as clinical proof-of-concept. Here we evaluate the immunosignature as a diagnostic for spontaneous canine lymphoma at both at initial diagnosis and evaluating the disease free interval following treatment.MethodsSera from dogs with confirmed lymphoma (B cell n = 38, T cell n = 11) and clinically normal dogs (n = 39) were analyzed. Serum antibody responses were characterized by analyzing the binding pattern, or immunosignature, of serum antibodies on a non-natural sequence peptide microarray. Peptides were selected and tested for the ability to distinguish healthy dogs from those with lymphoma and to distinguish lymphoma subtypes based on immunophenotype. The immunosignature of dogs with lymphoma were evaluated for individual signatures. Changes in the immunosignatures were evaluated following treatment and eventual relapse.ResultsDespite being a clonal disease, both an individual immunosignature and a generalized lymphoma immunosignature were observed in each dog. The general lymphoma immunosignature identified in the initial set of dogs (n = 32) was able to predict disease status in an independent set of dogs (n = 42, 97% accuracy). A separate immunosignature was able to distinguish the lymphoma based on immunophenotype (n = 25, 88% accuracy). The individual immunosignature was capable of confirming remission three months following diagnosis. Immunosignature at diagnosis was able to predict which dogs with B cell lymphoma would relapse in less than 120 days (n = 33, 97% accuracy).ConclusionWe conclude that the immunosignature can serve as a multilevel diagnostic for canine, and potentially human, lymphoma.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2407-14-657) contains supplementary material, which is available to authorized users.

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“…These studies used different proteomics approaches to identify not only biomarkers, but also prognostic protein expression patterns and proteins with relevance for various canine tumours. For instance, potentially relevant changes in the serum of dogs with high grade lymphomas or mammary tumours were reported (Gaines et al, 2007;Ratcliffe et al, 2009;Atherton et al, 2013;Mobasheri, 2013;Aresu et al, 2014;Zamani-Ahmadmahmudi et al, 2014). Furthermore, tear fluid analysis found changes in the amount of actin, albumin and an unidentified protein in dogs with different kinds of cancer (de Freitas Campos et al, 2008).…”

Section: Personalized Medicine In Veterinary Oncology: Where Are We?mentioning

confidence: 96%

Personalised medicine in veterinary oncology: One to cure just one

Klopfleisch

2015

The Veterinary Journal

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Proteomic identification and profiling of canine lymphoma patients

Cited by 32 publications

References 21 publications

Proteomic evaluation of sheep serum proteins

Proteomic evaluation of sheep serum proteins

The immunosignature of canine lymphoma: characterization and diagnostic application

Personalised medicine in veterinary oncology: One to cure just one

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