Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams

Souza, Carlos Eduardo A.; Rêgo, João Paulo Arcelino do; Lobo, Carlos Henrique; Oliveira, José T.A.; Nogueira, Fábio César Sousa; Domont, Gilberto B.; Fioramonte, Mariana; Gozzo, Fábio C.; Moreno, Frederico B.; Monteiro-Moreira, Ana Cristina de Oliveira; Figueiredo, J.R.; Moura, Arlindo A.

doi:10.1016/j.jprot.2012.05.039

Cited by 86 publications

(55 citation statements)

References 104 publications

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“…Within the criterion used to search trustworthy indicators of the animals reproductive potential are: the sexual behavior, the semen parameters, among others (SOUZA et al, 2012). However, these evaluations define some minimum criterion to the selection and utilization of the breeding (REGO, 2010).…”

Section: Introductionmentioning

confidence: 99%

Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep

Oliveira

Lima

et al. 2017

Rev. bras. saúde prod. anim.

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SUMMARY This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDITof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines.

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Section: Introductionmentioning

confidence: 99%

Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep

Oliveira

Lima

et al. 2017

Rev. bras. saúde prod. anim.

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“…Souza and coworkers detected 113 ± 7 spots per gel of cauda epididymal fluid from rams by means of 2D SDS-PAGE analysis (Souza et al, 2012). The most abundant proteins in these gels appeared as albumin and transferrin.…”

Section: Role Of Epididymal Proteome In Sperm Maturationmentioning

confidence: 99%

The epididymis and its role on sperm quality and male fertility

Souza¹,

Schorr-Lenz²,

Lucca³

et al. 2017

Anim Reprod

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The epididymis is a complex organ where spermatozoa acquire motility and ability to fertilize the egg. Epididymal maturation lasts 1 or 2 weeks and exposes the immature spermatozoa to a sequentially modified milieu, promoting intense interactions with secretions by the epididymal epithelium. Sperm surface modifications in response to interactions with epididymal secretions are key steps to achieve fertility ability. However, the precise molecular mechanisms that convert an immotile and infertile gamete into a highly motile cell capable of fusion with an oocyte are still unknown. Recent data on proteomics and transcriptomics of epididymal fluid and epididymosomes brought new ideas of the physiological roles of proteins and miRNAs in epididymal maturation in spermatozoa. This review focuses on the recent discoveries on epididymal fluid composition and its role on sperm maturation and preservation, linking to their survival and fertility potential.

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“…Phospholipid binding proteins belonging to the family of BSPs (Binder of Sperm Proteins) are present in the seminal plasma of several species, including bulls, bucks (male goats and rabbits), rams, men, male rodents, and stallions (Moura et al, 2007a;Manjunath et al, 2009;Souza et al, 2012;Plante et al, 2016). BSPs comprise approximately 60% of all proteins of the accessory sex gland fluid (Moura et al, 2007a) and seminal plasma (Manjunath and Sairam, 1987) from Bos taurus bulls and nearly the same amount in Bos indicus seminal plasma (Rego et al, 2014).…”

Section: Proteins Involved In Sperm Capacitationmentioning

confidence: 99%

“…Capacitating effects of BSPs seem to hold true in other species as well, such as mice and humans (Plante et al, 2014). However, while ruminant BSPs originate mainly from the accessory sex glands (Manjunath et al, 2009;Souza et al, 2012;van Tilburg et al, 2014), its expression in mice and humans occurs in the epidididymis.…”

Section: Proteins Involved In Sperm Capacitationmentioning

confidence: 99%

Functional aspects of seminal plasma and sperm proteins and their potential as molecularmarkers of fertility

Moura

Memili

2016

Anim Reprod

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Molecular components of sperm and in the media surrounding them influence bull fertility. Given this concept, proteins of the seminal plasma modulate crucial functions and events of reproduction, such as sperm motility and capacitation, cell protection, acrosome reaction, fertilization and embryonic development. Sperm proteins are also important for successful fertilization, egg activation and embryo development. Empirical associations between seminal and sperm proteins and fertility in the bovine indicate that these proteins are potential molecular markers of the male reproductive status.

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Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams

Cited by 86 publications

References 104 publications

Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep

Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep

The epididymis and its role on sperm quality and male fertility

Functional aspects of seminal plasma and sperm proteins and their potential as molecularmarkers of fertility

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