Proteomic analysis of the abdominal aortic aneurysm wall

Abstract

PurposesA ruptured AAA (rAAA) is a common cause of death in males over 60 years of age, and the global mortality from rAAA exceeds 80 %. The pathological processes occurring in the wall of the developing AAA are still unclear. The potential pathophysiological mechanisms underlying aortic aneurysms have been examined by many studies using immunohistochemistry and were, therefore, targeted at specific, preselected protein antigens.MethodsWe collected samples of tissue from anterior wall of an aneurysm sac from 15 patients indicated for AAA resection (group A) during the period from 2010 to 2011. These samples were subjected to a proteomic analysis. In addition, we collected control samples of identical aortic tissue from 10 heart-beating deceased organ donors (group B).ResultsA total of 417 differentially expressed protein fractions were identified, 18 of which were only detected in the healthy controls, while 85 were specific for aneurysm tissue and 314 were detectable in both groups. In 175 protein fractions, the gel-derived spot volumes differed significantly between aneurismal and healthy aortic tissue.ConclusionsWe found a significant difference in the proteome of the AAA tissue and non-dilated aortic tissue. We demonstrated that the AAA proteome is considerably richer and more varied than the healthy and atherosclerotic aorta. We believe that our results clearly demonstrate a completely different etiopathogenesis of atherosclerosis and aneurismal disease.

Age‐associated proteomic alterations in human aortic media

“…Therefore, comprehensive proteomic analyses are required to more completely understand vascular senescence. However, in humans, almost all the proteomic studies of the aorta have focused on arterial diseases, such as aortic aneurysm, [7][8][9][10][11][12] Marfan syndrome 13 and medial degeneration with aortic dilatation caused by aortic valve disease. 14 On the contrary, proteomic studies specifically examining chronological human *Current affiliation: Department of Medical Technology, School of Health Sciences, Tokyo University of Technology, Tokyo, Japan.…”

Abdominal Aortic Aneurysms Growth Is Associated With High Concentrations of Plasma Proteins in the Intraluminal Thrombus and Diseased Arterial Tissue

“…Only one study has focused on AAA-growth rates, 12 and most proteome studies of AAA and ILT samples have used pooled samples and included <15 individual samples. [13][14][15] No previous studies have attempted to use proteome analysis of separate samples from both the aneurismal wall and the corresponding thrombus; and previous results do therefore, to a large degree, reflect the ratio of ILT/wall of the analyzed material. In the present study, we use quantitative liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteome analysis of samples extracted from separated AAA wall and thrombus specimens from a group of electively operated patients in whom the expansion rate had been determined in the years preceding their surgical intervention.…”

Using Proteomics to Understand Abdominal Aortic Aneurysms: Where Are We?

“…a relatively ''healthy'' aorta obtained during organ removal from heart-beating deceased organ donors. They highlighted significant differences in the proteomes of the aortic aneurysm tissue and the non-dilated aortas with atherosclerosis [24].…”

“…Ig alpha-2 chain C region IGHA2_HUMAN [18,24] Plasma/serum biomarkers and their association with AAA size, expansion rate and rupture Plasma/serum: The depth of proteome analysis is severely limited in complex samples with a wide dynamic range of protein abundance such as plasma/serum. Pre-fractionation of a sample divides it into fractions of reduced complexity, allowing improved detection of lower abundance proteins.…”

“…In the proteomic map obtained by these authors, the expression level of most of the glycolytic enzymes was significantly reduced at the AAA site [23]. Recently, Molacek et al [24] carried out a proteomic analysis of the AAA wall and compared the findings with those of non-dilated wall tissue, i.e. a relatively ''healthy'' aorta obtained during organ removal from heart-beating deceased organ donors.…”