Proteomic Analysis of Kidney Preservation Solutions Prior to Renal Transplantation

Abstract

One of the main issues in kidney transplantation is the optimal functional preservation of the organ until its transplantation into the appropriate recipient. Despite intensive efforts, the functional preservation period remains limited to hours. During this time, as a result of cellular injury, various proteins, peptides, and other molecules are released by the organ into the preservation medium. In this study, we used proteomic techniques to analyze the protein profiles of preservation solutions in which organs had been preserved prior to their transplantation. Samples were obtained from the preservation solutions of 25 deceased donor kidneys scheduled for transplantation. The protein profiles of the solutions were analyzed using 2D gel electrophoresis/MALDI-TOF and LC-MS/MS. We identified and quantified 206 proteins and peptides belonging to 139 different groups. Of these, 111 proteins groups were belonging to kidney tissues. This study used proteomic techniques to analyze the protein profiles of organ preservation solutions. These findings will contribute to the development of improved preservation solutions to effectively protect organs for transplantation.

Proteomic analysis of machine perfusion solution from brain dead donor kidneys reveals that elevated complement, cytoskeleton and lipid metabolism proteins are associated with 1‐year outcome

“…Understanding these aspects in the context of donor organ function warrants further investigation. proteomics analysis performed by Coskun et al (2016), who found secretion of cytoskeletal proteins of DBD donor kidneys during SCS. However, they did not include transplant outcome into their analysis [24].…”

Heat shock proteins and kidney disease: perspectives of HSP therapy

“…Both HSP70 and HSP90 were rapidly induced in the cytoplasm of injured epithelial cells of the proximal tubules during the degenerative and regenerative phases of the post-ischemic injury. In the study of Coskun et al (2016), the members of HSP70 and HSP90 families were detected in the preservation solution of the pretransplanted kidney by proteomic analysis. Furthermore, the levels of HSP90β and HSP70 were correlated with ischemia time and donors' age.…”

Inflammaging and Complement System: A Link Between Acute Kidney Injury and Chronic Graft Damage

“…Furthermore, a strong association between chronic graft injury and overexpression of complement components has been found by proteomic analysis in kidney donor biopsies (62). These results indicated that shorter periods of ischemia are clearly associated with less complement activation; in addition, the protein profiles of preservation solutions in which kidney from deceased donors had been stored revealed intense activity of complement effectors (as C3, factor B) during organ storage preceding transplantation (63).…”