Proteomic analysis of <i>Taenia solium</i> metacestode excretion–secretion proteins

Victor, Björn; Kanobana, Kirezi; Gabriël, Sarah; Polman, Katja; Deckers, Nynke; Dorny, Pierre; Deelder, André M.; Palmblad, Magnus

doi:10.1002/pmic.201100496

Cited by 55 publications

(34 citation statements)

References 57 publications

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“…This revealed that a major 120 kDa complex was composed of precursor sub-units of 14 and 18 kDa that had a potential as biomarkers for differentiation of stages of neurocysticercosis. A later investigation, using LC and MS/MS of excretion-secretion proteins from T. solium metacestode from pigs has revealed a more extensive group of proteins [103]. A total of 76 proteins were identified, 27 of which were already identified as T. solium protein and a further 32 likely to be parasite derived but based on sequences from other helminths.…”

Section: Parasite Diseasesmentioning

confidence: 99%

The pig as an animal model for human pathologies: A proteomics perspective

Bassols

Costa

Eckersall

et al. 2014

Proteomics Clinical Apps

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Traditional biomedical models are easy to manage in experimental facilities and allow fast and affordable basic genetic studies related to human disorders, but in some cases they do not always represent the complexity of their physiology. Translational medicine demands selected models depending on the particularities of the human disease to be investigated, reproducing as closely as possible the evolution, clinical symptoms and molecular pathways, cells or tissues involved in the dysfunction. Thus, pig models offer an alternative because of their anatomical and physiological similarities to humans and the availability of genomic, transcriptomic and, progressively more, proteomic tools for analysis of this species. Furthermore, there is a wide range of natural, selected and transgenic porcine breeds. The present review provides a summary of the applications of the pig as a model for metabolic, cardiovascular, infectious diseases, xenotransplantation and neurological disorders and an overview of the possibilities that the diverse proteomic techniques offer to study these pathologies in depth.

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Section: Parasite Diseasesmentioning

confidence: 99%

The pig as an animal model for human pathologies: A proteomics perspective

Bassols

Costa

Eckersall

et al. 2014

Proteomics Clinical Apps

223

158

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“…The groups were further organized by Gene Ontology annotation information on biological process and molecular function. A total of 48 protein groups are labelled with an asterisk, indicating that they were also identified in the previous study (Additional file 2) [6]. For brevity, Table 1 shows only the 58 newly identified protein groups.…”

Section: Resultsmentioning

confidence: 93%

“…The in vitro production of the T. solium metacestode excretion/secretion proteins from Peru and Zambia at 24h and 48h and the generation of line spectra mzXML files have been previously described [6]. …”

Section: Methodsmentioning

confidence: 99%

“…These databases were fused into one final database. Database searching with X!Tandem (2010.10.01.1) [13] and subsequent analyses with PeptideProphet [14,15], iProphet [16] and ProteinProphet [17] were also performed as previously described [6]. All above mentioned tools, except transeq, are included with the Trans-Proteomic Pipeline v4.5 RAPTURE rev 2 [18].…”

Section: Methodsmentioning

confidence: 99%

“…Ideally, this database contains every protein likely to be in the sample, but obtaining such an all-including protein database proves difficult when there is little to no genomic information available, as was the case for T. solium until recently [5]. In our previous study, we bypassed this limitation by using a custom database with known proteins from related helminths ( Taenia , Echinococcus , Schistosoma and Trichinella ) as a target database in the LC-MS/MS experiments [6]. We deliberately did not use translated expressed sequence tags (ESTs), because we wanted to investigate to usefulness of a target database made up of protein sequences originating mostly from (closely) related helminths.…”

Section: Introductionmentioning

confidence: 99%

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Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins

et al. 2013

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BackgroundTaenia solium taeniasis/cysticercosis is a zoonotic helminth infection mainly found in rural regions of Africa, Asia and Latin America. In endemic areas, diagnosis of cysticercosis largely depends on serology, but these methods have their drawbacks and require improvement. This implies better knowledge of the proteins secreted and excreted by the parasite. In a previous study, we used a custom protein database containing protein sequences from related helminths to identify T. solium metacestode excretion/secretion proteins. An alternative or complementary approach would be to use expressed sequence tags combined with BLAST and protein mapping to supercontigs of Echinococcus granulosus, a closely related cestode. In this study, we evaluate this approach and compare the results to those obtained in the previous study.FindingsWe report 297 proteins organized in 106 protein groups based on homology. Additional classification was done using Gene Ontology information on biological process and molecular function. Of the 106 protein groups, 58 groups were newly identified, while 48 groups confirmed previous findings. Blast2GO analysis revealed that the majority of the proteins were involved in catalytic activities and binding.ConclusionsIn this study, we used translated expressed sequence tags combined with BLAST and mapping strategies to both confirm and complement previous research. Our findings are comparable to recent studies on other helminth genera like Echinococcus, Schistosoma and Clonorchis, indicating similarities between helminth excretion/secretion proteomes.

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A gel-free proteomic analysis of Taenia solium and Taenia crassiceps cysticerci vesicular extracts

et al. 2018

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The taeniasis/cysticercosis complex is a zoonosis caused by the presence of the parasite Taenia solium in humans. It is considered a neglected disease that causes serious public health and economic problems in developing countries. In humans, the most common locations for the larval form are the skeletal muscles, ocular system, and the central nervous system, which is the most clinically important. Several glycoproteins of T. solium and Taenia crassiceps cysticerci have been characterized and studied for their use in the immunodiagnosis of neurocysticercosis and/or the development of synthetic or recombinant vaccines against cysticercosis. The aim of this study was to perform a gel-free shotgun proteomic analysis to identify saline vesicular extract (SVE) proteins of T. solium and T. crassiceps cysticerci. After solubilization of the SVE with and without surfactant reagent and in-solution digestion, the proteins were analyzed by LC-MS/MS. Use of a surfactant resulted in a significantly higher number of proteins that were able to be identified by LC-MS/MS. Novel proteins were identified in T. solium and T. crassiceps SVE. The qualitative analysis revealed a total of 79 proteins in the Taenia species: 29 in T. solium alone, 11 in T. crassiceps alone, and 39 in both. These results are an important contribution to support future investigations and for establishing a Taenia proteomic profile to study candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.

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Proteomic analysis of Taenia solium metacestode excretion–secretion proteins

Cited by 55 publications

References 57 publications

The pig as an animal model for human pathologies: A proteomics perspective

The pig as an animal model for human pathologies: A proteomics perspective

Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins

A gel-free proteomic analysis of Taenia solium and Taenia crassiceps cysticerci vesicular extracts

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