Proteome of equine oviducal fluid: effects of ovulation and pregnancy

Smits, Katrien; Nelis, Hilde; Steendam, Katleen Van; Govaere, Jan; Roels, K.; Ververs, Cyrillus; Leemans, Bart; Wydooghe, Eline; Deforce, Dieter; Soom, Ann Van

doi:10.1071/rd15481

Cited by 33 publications

(40 citation statements)

References 75 publications

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“…Quantitative proteomics have further verified the hormonal regulation of bovine and ovine ODF proteins and have demonstrated gamete induced changes in the ODF proteome of pigs . Furthermore, the effect of the presence of an embryo on the ODF protein profile has been demonstrated in mares …”

Section: Introductionmentioning

confidence: 84%

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

Hackenbroch

Meyer

et al. 2019

Proteomics

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Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 h after intrauterine insemination. A workflow is used that integrates lectin affinity capture with stable‐isotope dimethyl labeling prior to nanoLC‐MS/MS analysis. In total, over 400 ODF proteins, including 214 lectin enriched glycoproteins, are identified and quantified. Selected data are validated by Western blot analysis. Spatiotemporal alterations in the abundance of ODF proteins in response to insemination are detected by global analysis. A subset of 63 potentially biologically relevant ODF proteins is identified, including extracellular matrix components, chaperones, oxidoreductases, and immunity proteins. Functional enrichment analysis reveals an altered peptidase regulator activity upon insemination. In addition to protein identification and abundance changes, N‐glycopeptide analysis further identifies 281 glycosites on 199 proteins. Taken together, these results show, for the first time, the evolving oviductal milieu early upon insemination. The identified proteins are likely those that modulate in vitro processes, including spermatozoa function.

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Section: Introductionmentioning

confidence: 84%

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

Hackenbroch

Meyer

et al. 2019

Proteomics

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“…Recently, our knowledge on the proteomic content of OF in mammals has greatly increased thanks to mass spectrometry. Proteomes of the tubal fluid have been obtained in pigs [35,36], dogs [37], sheep [38], cattle [39][40][41], horse [42], rabbit [43], and human [44]. The most abundant proteins in oviductal secretions include serum albumin, oviductin (OVGP1), heat shock proteins (HSP90B1, HSP90AB1, HSP90AA1, HSPA8, HSPA1B, HSPA5), annexins (ANXA1, ANXA4), tubulin subunits (TUBB5, TUBA4A), complement C3 (C3), and myosins (MYH9, MYH14) [38][39][40].…”

Section: Proteinsmentioning

confidence: 99%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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“…It has been demonstrated that the oviductal proteome changes with the different stages of the oestrous cycle (Lamy et al 2016; Soleilhavoup et al 2016), the presence of gametes (Georgiou et al 2005) or the presence of embryos (Smits et al 2017). The proteome of the oviduct epithelial cells has also been studied in humans (Wang et al 2016) and pigs (Seytanoglu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Genes Encoding Mammalian Oviductal Proteins Involved in Fertilization are Subjected to Gene Death and Positive Selection

Moros-Nicolás¹,

Fouchécourt²,

Goudet³

et al. 2018

J Mol Evol

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Oviductal proteins play an important role in mammalian fertilization, as proteins from seminal fluid. However, in contrast with the latter, their phylogenetic evolution has been poorly studied. Our objective was to study in 16 mammals the evolution of 16 genes that encode oviductal proteins involved in at least one of the following steps: (1) sperm–oviduct interaction, (2) acrosome reaction, and/or (3) sperm–zona pellucida interaction. Most genes were present in all studied mammals. However, some genes were lost along the evolution of mammals and found as pseudogenes: annexin A5 (ANXA5) and deleted in malignant brain tumor 1 (DMBT1) in tarsier; oviductin (OVGP1) in megabat; and probably progestagen-associated endometrial protein (PAEP) in tarsier, mouse, rat, rabbit, dolphin, and megabat; prostaglandin D2 synthase (PTGDS) in microbat; and plasminogen (PLG) in megabat. Four genes [ANXA1, ANXA4, ANXA5, and heat shock 70 kDa protein 5 (HSPA5)] showed branch-site positive selection, whereas for seven genes [ANXA2, lactotransferrin (LTF), OVGP1, PLG, S100 calcium-binding protein A11 (S100A11), Sperm adhesion molecule 1 (SPAM1), and osteopontin (SPP1)] branch-site model and model-site positive selection were observed. These results strongly suggest that genes encoding oviductal proteins that are known to be important for gamete fertilization are subjected to positive selection during evolution, as numerous genes encoding proteins from mammalian seminal fluid. This suggests that such a rapid evolution may have as a consequence that two isolated populations become separate species more rapidly.

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Proteome of equine oviducal fluid: effects of ovulation and pregnancy

Cited by 33 publications

References 75 publications

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Genes Encoding Mammalian Oviductal Proteins Involved in Fertilization are Subjected to Gene Death and Positive Selection

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