Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2

Stierum, Rob; Gaspari, Marco; Dommels, Yvonne E.M.; Ouatas, Taoufik; Pluk, Helma; Jespersen, Sonja; Vogels, Jack; Verhoeckx, Kitty; Groten, J.P.; Ommen, Ben van

doi:10.1016/s1570-9639(03)00204-8

Cited by 111 publications

(95 citation statements)

References 56 publications

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“…Proteomic analysis done by Friedman et al (18) showed low CKB levels in colorectal cancer. Moreover, CKB levels increase with differentiation of the colon cancer cell line Caco-2 (19). Other reports from proteomic analyses of differentially expressed proteins in colon cancer found similar low levels of CKB in tumor tissues.…”

Section: Discussionsupporting

confidence: 54%

Altered Expression and Localization of Creatine Kinase B, Heterogeneous Nuclear Ribonucleoprotein F, and High Mobility Group Box 1 Protein in the Nuclear Matrix Associated with Colon Cancer

Balasubramani

Day²,

Schoen

et al. 2006

Cancer Research

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Identification of biomarkers could lead to the development of effective screening tests for colorectal cancer. A previous study from our laboratory showed specific alterations of nuclear structure in colon cancer. In an effort to characterize these biomarkers, protein spots were selected from separations made by two-dimensional gel electrophoresis, which were analyzed by mass spectrometry. The sequences obtained from the isolated spots revealed that they have close similarity to creatine kinase B (CKB) isoforms, heterogeneous nuclear ribonucleoprotein F (hnRNP F) and high mobility group box 1 protein (HMGB1) isoforms. To determine the expression of these proteins in colon cancer, expression was studied in 9 tumor and matched adjacent normal pairs, 5 donor colons, 16 polyps, 4 metastatic liver lesions and matched adjacent normal pairs, and 3 liver donors. CKB and hnRNP F were expressed in 78% and 89% of colon tumors, respectively. hnRNP F had a higher frequency of expression than CKB in premalignant polyps. With the establishment of differential expression of the proteins in colon cancer, their subcellular localization was analyzed. The subcellular fractions studied both showed high protein levels of hnRNP F in colon tumors compared with normal colon tissues. Surprisingly, subcellular levels of CKB were decreased in colon tumors, suggesting that the observed high CKB levels in nuclear matrix extracts are caused by the enhanced localization of CKB to the nuclear matrix during colon tumorigenesis. These results suggest an involvement of hnRNP F and CKB in colorectal cancer. Additionally, they suggest that hnRNP F is a potential marker for colorectal cancer progression. (Cancer Res 2006; 66(2): 763-9)

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Section: Discussionsupporting

confidence: 54%

Altered Expression and Localization of Creatine Kinase B, Heterogeneous Nuclear Ribonucleoprotein F, and High Mobility Group Box 1 Protein in the Nuclear Matrix Associated with Colon Cancer

Balasubramani

Day²,

Schoen

et al. 2006

Cancer Research

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“…Upon differentiation, the adenocarcinoma cell line CaCo-2 appears to lose its tumorigenic phenotype and so represents a useful tool for examining tumor progression (38). We show that during this differentiation event, the U3 snoRNA and hU3-55K levels but not those of other box C/D snoRNAs and core snoRNP proteins are downregulated.…”

Section: Discussionmentioning

confidence: 84%

A Weak C′ Box Renders U3 snoRNA Levels Dependent on hU3-55K Binding

Knox

McKeegan

Debieux

et al. 2011

Molecular and Cellular Biology

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The rate of ribosome biogenesis, which is downregulated in terminally differentiated cells and upregulated in most cancers, regulates the growth rate and is linked to the cell's proliferative potential. The U3 box C/D small nucleolar RNP (snoRNP) is an integral component of the small subunit (SSU) processome and is essential for 18S rRNA processing. We show that U3 snoRNP assembly, and therefore U3 snoRNA accumulation, is regulated through the U3-specific protein hU3-55K. Furthermore, we report that the levels of several SSU processome components, including the U3 snoRNA but not other box C/D snoRNAs, are specifically downregulated during human lung (CaCo-2) and colon (CaLu-3) epithelial cell differentiation. c-Myc is reported to play an integral role in regulating ribosome production by controlling the expression of many ribosome biogenesis factors. Our data, however, indicate that this regulation is not dependent on c-Myc since the level of this protein does not change during epithelial cell differentiation. In addition, depletion of c-Myc had only a mild affect on the levels of SSU processome proteins. CaCo-2 cells are colon adenocarcinoma epithelial cells that are believed to revert to their precancerous state during differentiation. This suggests a significant increase in the levels of specific SSU processome components during tumorogenesis.

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“…This method produces a fraction of intact microvilli characterized by the presence of proteins such as annexin A2 (17,18), annexin A5 (19), ␣-enolase and creatine kinase B (20), phosphoglycerate kinase (21), cytosolic malate dehydrogenase (22), lactate-dehydrogenase (23), GST (20,24,25), catalase (24), peroxiredoxin (26), among others previously shown to localize to epithelia microvilli.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Characterization of Isolated Retinal Pigment Epithelium Microvilli

Bonilha

Bhattacharya

West

et al. 2004

Molecular & Cellular Proteomics

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Polarized epithelial cells are characterized by displaying compartmentalized functions associated with differential distribution of transporters, structural proteins, and signaling molecules on their apical and basolateral surfaces. Their apical surfaces frequently elaborate microvilli, which vary in structure according to the specific type and function of each epithelium. The molecular basis of this heterogeneity is poorly understood. However, differences in function will undoubtedly be reflected in the specific molecular composition of the apical surface in each epithelial subtype. We have exploited a method for isolating microvilli from the mouse eye using wheat germ agglutinin (WGA)-agarose beads to begin to understand the specific molecular composition of apical microvilli of the retinal pigment epithelium (RPE) and expand our knowledge of the potential function of this interface. Initially, apical RPE plasma membranes bound to WGA beads were processed for morphological analysis using known apical and basolateral surface markers. The protein composition of the apical microvilli was then established using proteomic analysis. Over 200 proteins were identified, including a number of proteins previously known to be localized to RPE microvilli, as well as others not known to be present at this surface. Localization of novel proteins identified with proteomics was confirmed by immunohistochemistry in both mouse and rat eye tissue. The data generated provides new information on the protein composition of the RPE apical microvilli. The isolation technique used should be amenable for isolating microvilli in other epithelia as well, allowing new insights into additional functions of this important epithelial compartment. Molecular & Cellular Proteomics 3:1119 -1127, 2004.Epithelial cells are characterized by the asymmetric distribution of proteins and lipids in their plasma membrane: a basic feature referred to as polarity. The functional polarity of epithelial cells is dependent on the asymmetric distribution of specific enzymes, signaling molecules, and transport proteins between their apical and basolateral surface membranes (1). The apical surface of cuboidal and columnar epithelial cells commonly faces a luminal cavity and is characterized by the presence of numerous surface membrane elaborations referred to as microvilli. Microvilli greatly increase the apical surface area and, consequently, the number of transport and signaling proteins it contains, thereby enhancing the epithelial functional capacity. Absorptive epithelia such as kidney and intestine have their apical surface decorated with highly organized apical microvilli of uniform length and width. More dynamic and less organized structures are present in the epithelial cells of the placenta and the retinal pigment epithelium (RPE), 1 which perform endocytosis and phagocytosis, respectively. The basis of this morphological heterogeneity is poorly understood and is likely to be related to the specialized function and specific molecular composition of the ...

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Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2

Cited by 111 publications

References 56 publications

Altered Expression and Localization of Creatine Kinase B, Heterogeneous Nuclear Ribonucleoprotein F, and High Mobility Group Box 1 Protein in the Nuclear Matrix Associated with Colon Cancer

Altered Expression and Localization of Creatine Kinase B, Heterogeneous Nuclear Ribonucleoprotein F, and High Mobility Group Box 1 Protein in the Nuclear Matrix Associated with Colon Cancer

A Weak C′ Box Renders U3 snoRNA Levels Dependent on hU3-55K Binding

Proteomic Characterization of Isolated Retinal Pigment Epithelium Microvilli

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