Proteoglycans and their roles in brain cancer

Wade, Anna; Robinson, Aaron E.; Engler, Jane R.; Petritsch, Claudia; James, C. David; Phillips, Joanna J.

doi:10.1111/febs.12109

Cited by 170 publications

(176 citation statements)

References 176 publications

(261 reference statements)

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…HS and CS thus may be able to act like a protection shield on the surface of cells (cancer and non-cancer), since they protrude out of the cell membrane like a brush [59,60], modulating cell phenotype, growth kinetics, invasiveness and metastatic potential [61][62][63]. Wade et al [64] reported for glioblastoma (GBM) a significant up-regulation of many proteoglycans with differences in the tumor subtypes. Also for melanoma an up-regulation of certain glycosaminoglycans was observed [65].…”

Section: Discussionmentioning

confidence: 99%

Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine

Riedl

Leber

Rinner

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Host defense-derived peptides have emerged as a novel strategy for the development of alternative anticancer therapies. In this study we report on characteristic features of human lactoferricin (hLFcin) derivatives which facilitate specific killing of cancer cells of melanoma, glioblastoma and rhabdomyosarcoma compared with non-specific derivatives and the synthetic peptide RW-AH. Changes in amino acid sequence of hLFcin providing 9-11 amino acids stretched derivatives LF11-316, -318 and -322 only yielded low antitumor activity. However, the addition of the repeat (di-peptide) and the retro-repeat (di-retro-peptide) sequences highly improved cancer cell toxicity up to 100% at 20 μM peptide concentration. Compared to the complete parent sequence hLFcin the derivatives showed toxicity on the melanoma cell line A375 increased by 10-fold and on the glioblastoma cell line U-87mg by 2-3-fold. Reduced killing velocity, apoptotic blebbing, activation of caspase 3/7 and formation of apoptotic DNA fragments proved that the active and cancer selective peptides, e.g. R-DIM-P-LF11-322, trigger apoptosis, whereas highly active, though non-selective peptides, such as DIM-LF11-318 and RW-AH seem to kill rapidly via necrosis inducing membrane lyses. Structural studies revealed specific toxicity on cancer cells by peptide derivatives with loop structures, whereas non-specific peptides comprised α-helical structures without loop. Model studies with the cancer membrane mimic phosphatidylserine (PS) gave strong evidence that PS only exposed by cancer cells is an important target for specific hLFcin derivatives. Other negatively charged membrane exposed molecules as sialic acid, heparan and chondroitin sulfate were shown to have minor impact on peptide activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine

Riedl

Leber

Rinner

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

show abstract

“…Both stabilized brain cancer cell lines and primary cells, obtained from biopsy of CSN tumors, can be used to form spheroids [196]. As frequently happens in the 3D world, neurospheres are more representative of the tumor with respect to the corresponding monolayer cultures.…”

Section: Tumor Brain Spheroids or Neurospheresmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

View full text Add to dashboard Cite

A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

show abstract

“…Структурная и функциональная роль ПГ в возник-новении и развитии злокачественных опухолей голов-ного мозга подробно обсуждается в обзорах [65,66]. Наиболее значимую роль при раке этого типа (глиаль-ные опухоли) играют ХСПГ/ДСПГ и кератансульфат-протеогликаны.…”

Section: протеогликаны в глиальных опухолях головного мозгаunclassified

Proteoglycans in normal physiology and carcinogenesis

Суховских¹,

Григорьева²

2018

Usp. mol. onkol

View full text Add to dashboard Cite

Proteoglycans and their roles in brain cancer

Cited by 170 publications

References 176 publications

Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine

Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Proteoglycans in normal physiology and carcinogenesis

Contact Info

Product

Resources

About