2014
DOI: 10.1111/acel.12296
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Abstract: Age-dependent neurodegenerative diseases progressively form aggregates containing both shared components (e.g., TDP-43, phosphorylated tau) and proteins specific to each disease. We investigated whether diverse neuropathies might have additional aggregation-prone proteins in common, discoverable by proteomics. Caenorhabditis elegans expressing unc-54p/Q40::YFP, a model of polyglutamine array diseases such as Huntington's, accrues aggregates in muscle 2–6 days posthatch. These foci, isolated on antibody-coupled… Show more

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Cited by 53 publications
(110 citation statements)
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“…Complexes containing those proteins were bound, rinsed thoroughly, and recovered by elution from the beads. The two immuno‐adsorbed subfractions as well as total large aggregates (prior to affinity isolation) were each partitioned by solubility in 1% sarcosyl to separate ‘compact’ (sarcosyl‐insoluble) aggregates from those that dissolved in this strong ionic detergent, in the presence of 0.3‐ m β‐mercaptoethanol as reducing agent (Ayyadevara et al ., 2014). A typical 1‐D acrylamide gel is shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Complexes containing those proteins were bound, rinsed thoroughly, and recovered by elution from the beads. The two immuno‐adsorbed subfractions as well as total large aggregates (prior to affinity isolation) were each partitioned by solubility in 1% sarcosyl to separate ‘compact’ (sarcosyl‐insoluble) aggregates from those that dissolved in this strong ionic detergent, in the presence of 0.3‐ m β‐mercaptoethanol as reducing agent (Ayyadevara et al ., 2014). A typical 1‐D acrylamide gel is shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Total aggregate protein far exceeds the sum of IP fractions, as is apparent in Fig. 1A (note lighter exposure for lanes 5 and 6), Table 1, and Supplemental Spreadsheets, reflecting the age‐dependent accrual of protein aggregates even without a pathogenic ‘seed’ protein (David et al ., 2010; Ayyadevara et al ., 2014). However, pronounced AD/NC bias was rare in total aggregates, where it was seen only for apolipoprotein E, neurochondrin, secernin‐1, and tau, with an intermediate shift also evident for filamins A and C, β‐SNAB, and carbonyl reductase.…”
Section: Resultsmentioning
confidence: 99%
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