Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood–Brain Barrier by Increasing Membrane Fluidity

Li, Chen; Chen, Linlin; Wang, Yuan‐Yuan; Wang, Tingting; Di, Dong; Zhang, Hao; Zhao, Huanhuan; Shen, Xu; Guo, J.

doi:10.2147/ijn.s291286

Cited by 20 publications

(18 citation statements)

References 50 publications

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“…Furthermore, the intracellular Ca 2+ increase might interfere with OP regulation via the release of PN-ion adsorption. According to the theory of double layers, divalent ions (Ca 2+ ) can replace monovalent ions (mainly intracellular K + ) when adsorbed by PNs, which rearranges PNion adsorption and regulates the effects of OP (45)(46)(47).…”

Section: Discussionmentioning

confidence: 99%

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

et al. 2021

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Cell swelling and membrane blebbing are characteristic of pyroptosis. In the present study, we explored the role of intracellular tension activity in the deformation of pyroptotic astrocytes. Protein nanoparticle-induced osmotic pressure (PN-OP) was found to be involved in cell swelling and membrane blebbing in pyroptotic astrocytes, and was associated closely with inflammasome production and cytoskeleton depolymerization. However, accumulation of protein nanoparticles seemed not to be absolutely required for pyroptotic permeabilization in response to cytoskeleton depolymerization. Gasdermin D activation was observed to be involved in modification of typical pyroptotic features through inflammasome-induced OP upregulation and calcium increment. Blockage of nonselective ion pores can inhibit permeabilization, but not inflammasome production and ion influx in pyroptotic astrocytes. The results suggested that the inflammasomes, as protein nanoparticles, are involved in PN-OP upregulation and control the typical features of pyroptotic astrocytes.

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Section: Discussionmentioning

confidence: 99%

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

et al. 2021

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“…Such behavior is well fit to an expression of occludin proteins in tissues as a reaction to osmotic stress due to depolymerization of microfilaments or microtubules. [67,68] Moreover, glycerol has been applied as an osmotic adjuvant to reduce intracranial hypertension. [69] It is important to note that in order to prevent the strong swelling of collagenous tissue under the action of OCA in ex vivo experiments, the treatment time must be <1 h. [70] A previous study of glycerol effect on the thermal properties of collagen using optical displacementenhanced heterodyne polarimeter shows improvement of thermal denaturation of collagen.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo confocal Raman microspectroscopy of porcinedura matersupported by optical clearing

Jaafar

Holomb

Sdobnov

et al. 2021

Journal of Biophotonics

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The effect of tissue optical clearing (TOC) to increase the probing depth and observe in‐depth structure of the ex vivo porcine dura mater was studied by confocal Raman microspectroscopy (CRM). Raman intensities were significantly increased at the depth of 250 μm for all collagen bands after treatment with glycerol. The influence of glycerol on collagen hydration was also investigated. The results indicate that the process of TOC can be divided into three main steps. The first one is a fast process of tissue dehydration accompanied by collagen shrinkage while the second relatively slow process is related to the glycerol penetration into the interfibrillar space of collagen combined with swelling of tissue. The third step is collagen dissociation caused by the high concentration of glycerol. To the best of our knowledge, this study is the first example to introduce the TOC technique in assisting CRM of ex vivo dura mater in‐depth probing.

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“…The plasmid encoding the FRET sensor was transfected into A549 cells according to a previously reported method [ 13 – 15 , 30 , 31 ]. The effectiveness of FRET in stable monoclonal cell lines depends on the dipole angle between donor-constitutively fluorescent enhanced cyan fluorescent protein (eCFP) and acceptor-constitutively fluorescent enhanced yellow fluorescent protein (eYFP).…”

Section: Methodsmentioning

confidence: 99%

“…Water metabolism and edema occurrence depend on transmembrane oncotic pressure gradients in the body [ 13 ]. Based on a recent study, protein nanoparticle production can result in increased osmotic pressure (PN-OP) and water flow, which is also thought to involve upregulation of membrane fluidity and promotes nonselective permeability [ 14 , 15 ]. Previous studies suggested that ARDS is associated with the secretion of proinflammatory cytokines and inflammasome production [ 1 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Protein nanoparticle-induced osmotic pressure gradients modify pulmonary edema through hyperpermeability in acute respiratory distress syndrome

et al. 2022

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Acute respiratory distress syndrome (ARDS), caused by noncardiogenic pulmonary edema (PE), contributes significantly to Coronavirus 2019 (COVID-19)-associated morbidity and mortality. We explored the effect of transmembrane osmotic pressure (OP) gradients in PE using a fluorescence resonance energy transfer-based Intermediate filament (IF) tension optical probe. Angiotensin-II- and bradykinin-induced increases in intracellular protein nanoparticle (PN)-OP were associated with inflammasome production and cytoskeletal depolymerization. Intracellular protein nanoparticle production also resulted in cytomembrane hyperpolarization and L-VGCC-induced calcium signals, which differed from diacylglycerol-induced calcium increment via TRPC6 activation. Both pathways involve voltage-dependent cation influx and OP upregulation via SUR1-TRPM4 channels. Meanwhile, intra/extracellular PN-induced OP gradients across membranes upregulated pulmonary endothelial and alveolar barrier permeability. Attenuation of intracellular PN, calcium signals, and cation influx by drug combinations effectively relieved intracellular OP and pulmonary endothelial nonselective permeability, and improved epithelial fluid absorption and PE. Thus, PN-OP is pivotal in pulmonary edema in ARDS and COVID-19, and transmembrane OP recovery could be used to treat pulmonary edema and develop new drug targets in pulmonary injury. Graphical Abstract

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Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood–Brain Barrier by Increasing Membrane Fluidity

Cited by 20 publications

References 50 publications

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis

Ex vivo confocal Raman microspectroscopy of porcinedura matersupported by optical clearing

Protein nanoparticle-induced osmotic pressure gradients modify pulmonary edema through hyperpermeability in acute respiratory distress syndrome

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