Protein-Mediated Biotemplating on the Nanoscale

Freeman, Amihay

doi:10.3390/biomimetics2030014

Cited by 14 publications

(12 citation statements)

References 91 publications

(94 reference statements)

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“…3,4 Unlike synthetic polymers that are characterized by structural inhomogeneities and non-uniform chain lengths/molecular weight distribution, 5 proteins are uniform in peptide length and molecular weight, resulting in biomaterials that are more precise in sequence, structure, and overall assemblies. 6 Protein-and peptide-based nanomaterials have been used for a myriad of biological applications, leveraging in large part their property to self-assemble. 1 Inspired by nature, several proteins/peptides have been engineered to self-assemble into a variety of complex structures, ranging from nanoparticles, vesicles, cages and fibrous assemblies; these can be endowed with novel functionalities offering numerous applications in diverse areas of bioengineering.…”

Section: Introductionmentioning

confidence: 99%

“…Using proteins as building blocks, it is now possible to create nanoscale architectures ranging from few nanometers to hundreds of nanometers . The unique properties of proteins including their modular nature, biocompatibility, and biodegradability offer exciting opportunities in designing smart nanomaterials. , Unlike synthetic polymers that are characterized by structural inhomogeneities and non-uniform chain lengths/molecular weight distribution, proteins are uniform in peptide length and molecular weight, resulting in biomaterials that are more precise in sequence, structure, and overall assemblies …”

Section: Introductionmentioning

confidence: 99%

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Self-Assembled Protein- and Peptide-Based Nanomaterials

Katyal

Meleties

Montclare

2019

ACS Biomater. Sci. Eng.

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Considerable effort has been devoted to generating novel protein-and peptide-based nanomaterials with their applications in a wide range of fields. Specifically, the unique property of proteins to self-assemble has been utilized to create a variety of nanoassemblies, which offer significant possibilities for next-generation biomaterials. In this minireview, we describe self-assembled protein-and peptide-based nanomaterials with focus on nanofibers and nanoparticles. Their applications in delivering therapeutic drugs and genes are discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Self-Assembled Protein- and Peptide-Based Nanomaterials

Katyal

Meleties

Montclare

2019

ACS Biomater. Sci. Eng.

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“… 50 Other types of biological 3D assemblies were used for the templating of complex inorganic structures, such as virus crystals, bacterial protein crystals, amyloid fibers, or virus-based nanowires. 51 …”

Section: Engineering Protein–nanomaterials Hybrids Based On Ctpr Scaffoldsmentioning

confidence: 99%

Engineered Repeat Protein Hybrids: The New Horizon for Biologic Medicines and Diagnostic Tools

et al. 2021

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Conspectus The last decades have witnessed unprecedented scientific breakthroughs in all the fields of knowledge, from basic sciences to translational research, resulting in the drastic improvement of the lifespan and overall quality of life. However, despite these great advances, the treatment and diagnosis of some diseases remain a challenge. Inspired by nature, scientists have been exploring biomolecules and their derivatives as novel therapeutic/diagnostic agents. Among biomolecules, proteins raise much interest due to their high versatility, biocompatibility, and biodegradability. Protein binders (binders) are proteins that bind other proteins, in certain cases, inhibiting or modulating their action. Given their therapeutic potential, binders are emerging as the next generation of biopharmaceuticals. The most well-known example of binders are antibodies, and inspired by them researchers have developed alternative binders using protein design approaches. Protein design can be based on naturally occurring proteins in which, by means of rational design or combinatorial approaches, new binding interfaces can be engineered to obtain specific functions or based on de novo proteins emerging from state-of-the-art computational methodologies. Among the novel designed proteins, a class of engineered repeat proteins, the consensus tetratricopeptide repeat (CTPR) proteins, stand out due to their stability and robustness. The CTPR unit is a helix-turn-helix motif constituted of 34 amino acids, of which only 8 are essential to ensure correct folding of the structure. The small number of conserved residues of CTPR proteins leaves plenty of freedom for functional mutations, making them a base scaffold that can be easily and reproducibly tailored to endow desired functions to the protein. For example, the introduction of metal-binding residues (e.g., histidines, cysteines) drives the coordination of metal ions and the subsequent formation of nanomaterials. Additionally, the CTPR unit can be conjugated with other peptides/proteins or repeated in tandem to encode larger CTPR proteins with superhelical structures. These properties allow for the design of both binder and nanomaterial-coordination modules as well as their combination within the same molecule, making the CTPR proteins, as we have demonstrated in several recent examples, the ideal platform to develop protein–nanomaterial hybrids. Generally, the fusion of two distinct materials exploits the best properties of each; however, in protein–nanomaterial hybrids, the fusion takes on a new dimension as new properties arise. These hybrids have ushered the use of protein-based nanomaterials as biopharmaceuticals beyond their original therapeutic scope and paved the way for their use as theranostic agents. Despite several reports of protein-stabilized nanomaterials found in the literature, these systems offer limited control in the synthesis and properties of the grown nanomaterials, as the protein acts just as a stabilizing agent with no significant functional contribution. T...

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“…Proteins have been used in many studies to fabricate materials such as nanotubes or nanospheres since they are naturally able to display catalytic and structural functions and are biocompatible and biodegradable. Protein crystals are of great interest as well, as their structures allow for the development of 3D crystalline composites with potential applications in different fields [8,9]. Self-assembly of biological molecules is one of the characteristics that scientists are most interested in.…”

Section: Introductionmentioning

confidence: 99%

Bio-Templating: An Emerging Synthetic Technique for Catalysts. A Review

et al. 2021

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In the last few years, researchers have focused their attention on the synthesis of new catalyst structures based on or inspired by nature. Biotemplating involves the transfer of biological structures to inorganic materials through artificial mineralization processes. This approach offers the main advantage of allowing morphological control of the product, as a template with the desired morphology can be pre-determined, as long as it is found in nature. This way, natural evolution through millions of years can provide us with new synthetic pathways to develop some novel functional materials with advantageous properties, such as sophistication, miniaturization, hybridization, hierarchical organization, resistance, and adaptability to the required need. The field of application of these materials is very wide, covering nanomedicine, energy capture and storage, sensors, biocompatible materials, adsorbents, and catalysis. In the latter case, bio-inspired materials can be applied as catalysts requiring different types of active sites (i.e., redox, acidic, basic sites, or a combination of them) to a wide range of processes, including conventional thermal catalysis, photocatalysis, or electrocatalysis, among others. This review aims to cover current experimental studies in the field of biotemplating materials synthesis and their characterization, focusing on their application in heterogeneous catalysis.

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Protein-Mediated Biotemplating on the Nanoscale

Cited by 14 publications

References 91 publications

Self-Assembled Protein- and Peptide-Based Nanomaterials

Self-Assembled Protein- and Peptide-Based Nanomaterials

Engineered Repeat Protein Hybrids: The New Horizon for Biologic Medicines and Diagnostic Tools

Bio-Templating: An Emerging Synthetic Technique for Catalysts. A Review

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