Protein Kinase C (PKC) Signal Transduction System Involved in Larval Metamorphosis of the Barnacle, Balanus amphitrite

Yamamoto, Hisashi; Tachibana, Akiko; Matsumura, Keisuke; Fusetani, Nobuhiro

doi:10.2108/zsj.12.391

Cited by 58 publications

(35 citation statements)

References 13 publications

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“…Furthermore, our observations are also supported by some previous studies indicating that the reduction of NOS activity accelerated larval metamorphosis in the ascidian Ciona intestinalis (Comes et al, 2007) and the gastropod Crepidula fornicate (Pechenik et al, 2007). In contrast to previous findings regarding the role of PKC during B. amphitrite settlement (Yamamoto et al, 1995), we did not observe metamorphosis of any cyprid without attachment for all of NO level manipulations, suggesting NO signaling may control the overall settlement process in this species.…”

Section: Discussionsupporting

confidence: 91%

“…In particular, the involvement of pheromones, hormones, neurotransmitters and proteins in regulating larval settlement of B. amphitrite has been intensively studied (e.g. Clare et al, 1995;Yamamoto et al, 1995;Yamamoto et al, 1997;Matsumura et al, 1998b;Matsumura et al, 1998c;Yamamoto et al, 1999;Delort et al, 2000;Endo et al, 2009;Gallus et al, 2010). Based on these results, it has been suggested that signaling transduction systems are likely involved in transmitting and/or translating these exogenous signals to endogenous effectors, resulting in the initiation of larval settlement (Clare et al, 1995;Yamamoto et al, 1997;Yamamoto et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…In a consecutive series of publications, Yamamoto and colleagues concluded that the protein kinase C (PKC) pathway might play an important role during larval metamorphosis but not during attachment of B. amphitrite (Yamamoto et al, 1995;Yamamoto et al, 1997). The same group of scientists exposed the cyprid larvae to serotonin, serotonin uptake blocker and serotonin antagonists, and postulated that serotonin may be involved in regulating the overall settlement process, which includes both attachment and metamorphosis (Yamamoto et al, 1996;Yamamoto et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacleBalanus (=Amphibalanus) amphitrite

Zhang

et al. 2012

Journal of Experimental Biology

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SUMMARYThe barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling. Supplementary material available online at

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacleBalanus (=Amphibalanus) amphitrite

Zhang

et al. 2012

Journal of Experimental Biology

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“…However, pharmacological assays have provided evidence that, after pheromone recognition, the signal transduction pathway in cyprids involves cAMP (Rittschof et al 1986;Clare et al 1995). Also, studies suggest that protein kinase C (PKC) participates in the signal transduction pathway leading to metamorphosis since cyprids treated with PKC activators metamorphosed without prior attachment (Yamamoto et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Settlement of the barnacleBalanus improvisus: The roles of dopamine and serotonin

Zega

Pennati

Dahlström

et al. 2007

Italian Journal of Zoology

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The regulation of the settlement process in barnacles has attracted research interest due to their role as fouling organisms. The involvement of neurotransmitters in the regulation of settlement of marine invertebrate larvae has been described in several species. In this work, we reported the effects of the neurotransmitters dopamine and serotonin on the settlement of cyprids of the barnacle Balanus improvisus and described differences among cyprids of different ages. Also, we tested the effects of dopaminergic and serotonergic agonists and antagonists on settlement of cyprid larvae. We found that dopamine significantly stimulated settlement of 2-and 4-day-old cyprids, while serotonin exerted an inhibitory effect, regardless of cyprid age. The agonists and antagonists to the two neurotransmitters were not able to stimulate settlement but in general had either an inhibitory or no effect. We compared our results to those previously reported of the roles of dopamine and serotonin in the settlement of Balanus amphitrite. There appeared to be striking differences in the effects of these neurotransmitters between the two species because it has been reported that serotonin induces settlement in B. amphitrite and that dopamine inhibits it. This suggested that dopamine and serotonin play pivotal roles in settlement of barnacle but may act in different ways in the two species.

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“…Larval settlement assays to detect the inducing or inhibiting effect of various substances or substrates on this species have been conducted by several authors (Rittschof et al, 1984(Rittschof et al, , 1992Kon-ya and Miki, 1994;Kitamura et al, 1995;Yamamoto et al, 1995;Satuito et al, 1996;Matsumura et al, 1998 a, b, c;Mishra et al, 1998;Kitamura, 1999;Mishra and Kitamura, 2000). The larval development of both Megabalanus volcano and M. rosa under laboratory conditions has been described (Kado and Hirano, 1994;Okano et al, 1996), but no settlement assay method for these barnacles has yet been reported.…”

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confidence: 99%

Laboratory culture of Balanus trigonus larvae by the method of B. amphitrite for establishing a multi-species settlement assay.

2001

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In the study of antifouling, it is important to establish a method for culturing barnacle larvae for settlement assays. In the laboratory, the diatom Chaetoceros gracilis has been shown to be one of the best diets for raising the larvae of Balanus amphitrite. We applied this culture method to B. trigonus, supplying of rate of development to the cyprid stage, and the survival rates at different food concentrations, indicated survival rate on the sixth day following the start of culture was 84%, and about 71% of the larvae had developed to cyprids by then. Parallel observations on larvae of B. amphitrite showed that at its optimal trigonus. In settlement assays using cyprids of each species and six-well multiwell plates, the percentage established for B. amphitrite can also be applied to B. trigonus.Key words: Balanus trigonus, food concentration, Chaetoceros gracilis, larval assayIn the study of antifouling, the barnacle Balanus amphitrite has proven to be an excellent model organism because of its rapid larval development and the ease of rearing the larvae in synchronous mass culture (Rittschof et al., 1992). Larval settlement assays to detect the inducing or inhibiting effect of various substances or substrates on this species have been conducted by several authors (Rittschof et al., 1984(Rittschof et al., , 1992Kon-ya and Miki, 1994;Kitamura et al., 1995;Yamamoto et al., 1995;Satuito et al., 1996; Matsumura et al., 1998 a, b, c;Mishra et al., 1998;Kitamura, 1999;Mishra and Kitamura, 2000). The larval development of both Megabalanus volcano and M. rosa under laboratory conditions has been described (Kado and Hirano, 1994;Okano et al., 1996), but no settlement assay method for these barnacles has yet been reported. Establishing multispecies settlement assay methods will no doubt provide a means for gaining information useful in antifouling studies.In the present study, we focused on Balanus trigonus for establishing larval culture and settlement assay methods because this animal belongs to the same genus as B. amphitrite and settles in close proximity to B.amphitrite in our study area. In order to culture the larvae of two or three species of barnacles at the same time in the laboratory, it would be convenient if we could simply adopt the already established culture method of B.

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Protein Kinase C (PKC) Signal Transduction System Involved in Larval Metamorphosis of the Barnacle, Balanus amphitrite

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 58 publications

References 13 publications

The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacleBalanus (=Amphibalanus) amphitrite

The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacleBalanus (=Amphibalanus) amphitrite

Settlement of the barnacleBalanus improvisus: The roles of dopamine and serotonin

Laboratory culture of Balanus trigonus larvae by the method of B. amphitrite for establishing a multi-species settlement assay.

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