Protein Expression Profile of the Mouse Metaphase-II Oocyte

Abstract

The mature oocyte contains the full complement of maternal proteins required for fertilization, the transition to zygotic transcription, and the beginning stages of embryogenesis. Many of these proteins have yet to be characterized. In this study, two-dimensional electrophoresis (2-DE) of mouse metaphase-II (MII) oocyte proteins, stained with silver staining or Pro-Q Diamond dye, was performed to describe the proteome and phosphoproteome of the mouse oocyte derived from ICR mice. A total of 869 selected protein spots, corresponding to 380 unique proteins, were identified successfully by mass spectrometry, in which 90 protein spots representing 53 unique proteins have been stained with Pro-Q Diamond, indicating that they are in phosphorylated forms. All identified proteins were bioinformatically annotated in detail and compared with the embryonic stem cell (ESC) proteome. A proteome reference database for the mouse oocyte was established from the protein data generated in this study, which can be accessed over the Internet ( http://reprod.njmu.edu.cn/2d). This database is the most detailed mouse oocyte proteomic database to date. It should be valuable in expanding our knowledge of the regulation of signaling in oogenesis, fertilization, and embryo development, while revealing potential mechanisms for epigenetic reprogramming.

Identification and Characterization of an Oocyte Factor Required for Porcine Nuclear Reprogramming

“…During the past decades, several approaches analyzing the protein composition of oocytes have been conducted to obtain the proteome signatures of Xenopus, bovine, pig, and mouse oocytes (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). Calvert et al (15) identified eight highly abundant heat shock proteins and related chaperones in the mature mouse egg by two-dimensional difference gel electrophoresis (DIGE).…”

Derivation and application of pluripotent stem cells for regenerative medicine

“…It was also reported that some of the placental abnormality of nuclear transfer embryos can be blamed on the abnormal expression of some genes, such as HSPC117 . Furthermore, protein profile of the mouse metaphase-II oocyte was analyzed, which might be valuable in revealing potential mechanisms of epigenetic reprogramming in nuclear transfer (Ma et al, 2008).…”

OMICS for the Identification of Biomarkers for Oocyte Competence, with Special Reference to the Mare as a Prospective Model for Human Reproductive Medicine

“…In a subsequent study by Ma et al, (2008), two-dimensional electrophoresis of mouse metaphase-II (MII) ooplasmic proteins (the ZP was removed by digestion before protein extraction) was performed to describe the proteome and phosphoproteome of oocytes derived from ICR mice. A total of 869 selected protein spots, corresponding to 380 unique proteins, were identified successfully by mass spectrometry.…”

“…Studies in farm animals, such as cattle and pigs, have been performed for both qualitative database generation and for quantification of proteome changes during oocyte IVM (bovine: Coenen et al, 2004;Bhojwani et al, 2006;Massicotte et al, 2006;Memili et al, 2007;Berendt et al, 2009;pig: Ellederova et al, 2004;Susor et al, 2007, reviewed by Ma et al, 2008;Arnold & Frohlich, 2011). Berendt et al (2009) performed two-dimensional gel electrophoresis saturation labeling to detect quantitative differences in the proteomes of immature versus IVM-MII bovine oocytes.…”