Protein Expression Changes in Maize Roots in Response to Humic Substances

Carletti, Paolo; Masi, Antonio; Spolaore, Barbara; Laureto, Patrizia Polverino de; Zorzi, Mariangela De; Turetta, Loris; Ferretti, M.; Nardi, Serenella

doi:10.1007/s10886-008-9477-4

Cited by 51 publications

(34 citation statements)

References 46 publications

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“…Effects of humic substances on plant genes and their metabolites have been further characterized in reports using protemotics, trancriptomics, and microarrays. Proteomic analyses of maize roots following applications of humic substances revealed that a total of 42 proteins were differentially expressed by HS, including proteins related to energy, metabolism, and cellular transport (Carletti et al 2008). This study concluded that the major pathways in the roots affected by humic substances were sucrose metabolism, malate dehydrogenase, ATPases, and cytoskeletal proteins.…”

Section: Plant Physiology and Metabolismmentioning

confidence: 99%

Agricultural uses of plant biostimulants

2014

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Background Plant biostimulants are diverse substances and microorganisms used to enhance plant growth. The global market for biostimulants is projected to increase 12 % per year and reach over $2,200 million by 2018. Despite the growing use of biostimulants in agriculture, many in the scientific community consider biostimulants to be lacking peer-reviewed scientific evaluation. Scope This article describes the emerging definitions of biostimulants and reviews the literature on five categories of biostimulants: i. microbial inoculants, ii. humic acids, iii. fulvic acids, iv. protein hydrolysates and amino acids, and v. seaweed extracts.Conclusions The large number of publications cited for each category of biostimulants demonstrates that there is growing scientific evidence supporting the use of biostimulants as agricultural inputs on diverse plant species. The cited literature also reveals some commonalities in plant responses to different biostimulants, such as increased root growth, enhanced nutrient uptake, and stress tolerance.

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Section: Plant Physiology and Metabolismmentioning

confidence: 99%

Agricultural uses of plant biostimulants

2014

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“…Carletti et al [47] reported for the first time a proteomic approach in a study on maize seedlings treated with HS. These authors isolated plasma membrane proteins, including triosophosphate isomerase (TIM; EC 5.3.1.1), glyceraldehyde-3-P dehydrogenase (GAPD; EC 1. , and verified whether these were downregulated by the HS treatment.…”

Section: Changes On Primary and Secondary Metabolismmentioning

confidence: 99%

Physiological responses to humic substances as plant growth promoter

Canellas

Olivares

2014

Chem Biol Techn Agric

373

243

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Humic substances (HS) have been widely recognized as a plant growth promoter mainly by changes on root architecture and growth dynamics, which result in increased root size, branching and/or greater density of root hair with larger surface area. Stimulation of the H + -ATPase activity in cell membrane suggests that modifications brought about by HS are not only restricted to root structure, but are also extended to the major biochemical pathways since the driving force for most nutrient uptake is the electrochemical gradient across the plasma membrane. Changes on root exudation profile, as well as primary and secondary metabolism were also observed, though strongly dependent on environment conditions, type of plant and its ontogeny. Proteomics and genomic approaches with diverse plant species subjected to HS treatment had often shown controversial patterns of protein and gene expression. This is a clear indication that HS effects of plants are complex and involve non-linear, cross-interrelated and dynamic processes that need be treated with an interdisciplinary view. Being the humic associations recalcitrant to microbiological attack, their use as vehicle to introduce beneficial selected microorganisms to crops has been proposed. This represents a perspective for a sort of new biofertilizer designed for a sustainable agriculture, whereby plants treated with HS become more susceptible to interact with bioinoculants, while HS may concomitantly modify the structure/activity of the microbial community in the rhizosphere compartment. An enhanced knowledge of the effects on plants physiology and biochemistry and interaction with rhizosphere and endophytic microbes should lead to achieve increased crop productivity through a better use of HS inputs in Agriculture.

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“…This reductionist assay tests for a protein's ability to form an ionic conductance and has been used successfully to address animal annexin transport function (e.g., Burger et al, 1994;Liemann et al, 1996). Phosphatidylethanolamine was used to form bilayers as it is a predominant phospholipid in maize plasma membrane (Bohn et al, 2001;Kukavica et al, 2007) where ANN33 and ANN35 are located (Carletti et al, 2008). Phosphatidylserine was incorporated because annexin binding to phosphatidylserine is a hallmark of this protein family, and cholesterol was used to provide bilayer stability under the ionic conditions used.…”

Section: Highly Purified Zm Ann33/35 Preparation Elevates [Ca 2+ ] Cytmentioning

confidence: 99%

“…This protoplast assay requires annexin addition to the extracellular plasma membrane face. While ANN33/35 are soluble proteins, they also exist in the maize plasma membrane (Hochholdinger et al, 2005;Carletti et al, 2008) and are predicted to be secreted (SecretomeP program; Bendtsen et al, 2004). Thus, the protoplast assay also tests for annexin interaction with a plasma membrane from the extracellular face.…”

Section: Introductionmentioning

confidence: 99%

Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance

et al. 2009

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Regulation of reactive oxygen species and cytosolic free calcium ([Ca 2+ ] cyt ) is central to plant function. Annexins are small proteins capable of Ca 2+ -dependent membrane binding or membrane insertion. They possess structural motifs that could support both peroxidase activity and calcium transport. Here, a Zea mays annexin preparation caused increases in [Ca 2+ ] cyt when added to protoplasts of Arabidopsis thaliana roots expressing aequorin. The pharmacological profile was consistent with annexin activation (at the extracellular plasma membrane face) of Arabidopsis Ca 2+ -permeable nonselective cation channels. Secreted annexins could therefore modulate Ca 2+ influx. As maize annexins occur in the cytosol and plasma membrane, they were incorporated at the intracellular face of lipid bilayers designed to mimic the plasma membrane. Here, they generated an instantaneously activating Ca 2+ -permeable conductance at mildly acidic pH that was sensitive to verapamil and Gd 3+ and had a Ca 2+ -to-K + permeability ratio of 0.36. These results suggest that cytosolic annexins create a Ca 2+ influx pathway directly, particularly during stress responses involving acidosis. A maize annexin preparation also demonstrated in vitro peroxidase activity that appeared independent of heme association. In conclusion, this study has demonstrated that plant annexins create Ca 2+ -permeable transport pathways, regulate [Ca 2+ ] cyt , and may function as peroxidases in vitro.

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Protein Expression Changes in Maize Roots in Response to Humic Substances

Cited by 51 publications

References 46 publications

Agricultural uses of plant biostimulants

Agricultural uses of plant biostimulants

Physiological responses to humic substances as plant growth promoter

Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance

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