Protein expression by Aeromonas hydrophila during growth in vitro and in vivo

Immune responses of resistant and sensitive common carp families following experimental challenge with Aeromonas hydrophila

“…Septicaemia can spread among cultured fish very quickly resulting in high mortality rates [3]. A. hydrophila is extremely heterogeneous and there is currently no commercially available vaccine that is effective against all A. hydrophila strains [4]. Antibiotics and chemotherapeutic agents are often used to prevent or treat fish diseases of bacterial origin, but they can accumulate in the fish meat and in the aquatic environment, and there is an increasing risk of developing antibiotic resistant strains of bacteria.…”

Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media

“…Therefore, recent efforts have been aimed at identifying specific bacterial antigens to target for vaccine development. Such research has resulted in the identification of numerous proteins or specific fractions of F. psychrophilum (Rahman et al 2002, Merle et al 2003, LaFrentz et al 2004, Massias et al 2004, Crump et al 2005, Dumetz et al 2007, 2008, LaFrentz 2007, Sudheesh et al 2007), some of which have been shown to be protective (Rahman et al 2002, LaFrentz et al 2004, Dumetz et al 2006, Crump et al 2007). These studies demonstrate the potential for identifying vaccine candidates; however, the work was performed using in vitro cultured bacteria, and our knowledge of differential gene expression by F. psychrophilum grown in vivo is lacking.…”

“…Brown et al (1988) suggested that bacteria grown in vivo should be analyzed in studies aimed at identifying virulence factors and vaccine candidate antigens, because expression of these factors may be altered in the in vitro environment. Several studies have taken this approach and identified differences in bacterial pathogens by comparing cells grown in vitro and in vivo, including differences in carbohydrate components (Thornton et al 1993, Wang et al 2004, Poobalane et al 2008) and protein regulation (Thornton et al 1993, Colquhoun & Sorum 1998, Bakopoulos et al 2004, Ebanks et al 2004, Poobalane et al 2008.…”

MHC class IIα alleles associated with resistance to Aeromonas hydrophila in purse red common carp, Cyprinus carpio Linnaeus

“…Aeromonas hydrophila can cause fish motile aeromonad septicaemia (MAS), and there are no effective vaccines available against it (Poobalane et al . ). Traditionally, antibiotics have been used to prevent and treat bacterial diseases, but they can accumulate in fish and in the environment.…”