Protein encapsulation by electrospinning and electrospraying

Moreira, Anabela; Lawson, Daniel J.; Onyekuru, Lesley; Dziemidowicz, Karolina; Angkawinitwong, Ukrit; Costa, Pedro F.; Radacsi, Norbert; Williams, Gareth R.

doi:10.1016/j.jconrel.2020.10.046

Cited by 75 publications

(55 citation statements)

References 198 publications

(254 reference statements)

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Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

“…Another valuable advantage of electrohydrodynamic techniques is how they enable the encapsulation of bioactive elements with distinct chemical structures, including not only small molecules but also DNA [ 290 , 291 ] and proteins [ 285 , 292 , 293 ]. Cell electrospinning has also been performed, allowing the direct incorporation of live cells during the scaffold manufacturing process [ 294 ].…”

Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

“…The organisation of the deposited fibres can be controlled using different collector architectures: a flat collector (e) will generate randomly deposited fibres, while a cylindrical mandrel (f) rotating at high speeds will result in highly aligned fibres. The Taylor cone schematic representation in (b) was adapted from [285] with permission from Elsevier. Copyright © 2020, A programmable syringe pump is used to infuse a polymer solution, emulsion, or melt through an electrically conductive spinneret (e.g., a metallic needle), to which a high voltage (usually 5-20 kV) is applied.…”

Section: Electrospinningmentioning

confidence: 99%

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Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Gonçalves¹,

Moreira²,

Weber

et al. 2021

Pharmaceutics

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The socioeconomic impact of osteochondral (OC) damage has been increasing steadily over time in the global population, and the promise of tissue engineering in generating biomimetic tissues replicating the physiological OC environment and architecture has been falling short of its projected potential. The most recent advances in OC tissue engineering are summarised in this work, with a focus on electrospun and 3D printed biomaterials combined with stem cells and biochemical stimuli, to identify what is causing this pitfall between the bench and the patients’ bedside. Even though significant progress has been achieved in electrospinning, 3D-(bio)printing, and induced pluripotent stem cell (iPSC) technologies, it is still challenging to artificially emulate the OC interface and achieve complete regeneration of bone and cartilage tissues. Their intricate architecture and the need for tight spatiotemporal control of cellular and biochemical cues hinder the attainment of long-term functional integration of tissue-engineered constructs. Moreover, this complexity and the high variability in experimental conditions used in different studies undermine the scalability and reproducibility of prospective regenerative medicine solutions. It is clear that further development of standardised, integrative, and economically viable methods regarding scaffold production, cell selection, and additional biochemical and biomechanical stimulation is likely to be the key to accelerate the clinical translation and fill the gap in OC treatment.

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Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

Section: Osteochondral Tissue Engineeringmentioning

confidence: 99%

Section: Electrospinningmentioning

confidence: 99%

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Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Gonçalves¹,

Moreira²,

Weber

et al. 2021

Pharmaceutics

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“…To help achieve the production of healthier food, numerous techniques and methodologies have been developed within the last couple decades following the nutrient fortification (Akhtar et al, 2011;Shilpashree et al, 2015;Zhu et al, 2020) or encapsulation path when components sensitive to processing (i.e., vitamins, antioxidants, enzymes, probiotics) are incorporated (Ye et al, 2018;Maurya et al, 2020;Castro Coelho et al, 2021). For the encapsulation of bioactive ingredients, several technologies have been proposed including nano/microemulsification, spray drying, lyophilization, electro-spinning/ spraying (Ray et al, 2016;Jalali-Jivan et al, 2020;Moreira et al, 2020). However, micro-emulsions are considered more advantageous than nano-emulsions since they are transparent and therefore do not affect the appearance of food products nor they require expensive equipment or ingredients for their formation McClements, 2018).…”

Section: Introductionmentioning

confidence: 99%

Fortification of chocolate usingMoringa oleiferaextract encapsulated in microemulsions

Kaltsa

Alibade

Batra

et al. 2021

OCL

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The aim of the present study was to evaluate the physical and antioxidant properties of microemulsions containing Moringa oleifera leaf extract (MLE) produced by the means of a deep eutectic solvent. Selected microemulsions containing MLE were incorporated in chocolate products to enrich them. Their color properties including CIE L*, a*, b* parameters and whitening index (WI) along with DPPH radical scavenging activity were assessed during a period of 8 months. The antioxidant activity of microemulsions depended on the oil phase used, while it was unaffected by the concentration of MLE. Samples prepared with soybean oil as oil phase containing MLE presented the highest radical inhibition percentage (I% = 26.8–27.8%). Coconut microemulsions were finally incorporated at 2 and 4% w/w concentration into chocolate products, as coconut oil is a known cocoa butter substitute. Although the incorporation of MLE microemulsions did not affect the color properties of most of the chocolates, enriched products did not exhibit superior antioxidant activity compared to control samples.

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“…The suitability of EHD processing for protein encapsulation has been proven with many biologics (recently reviewed in refs. [ 29 , 30 ]), including bovine serum albumin [ 31 ], growth factors [ [32] , [33] , [34] ], hormones [ 35 ], and enzymes such as lysozyme [ 36 ] and alkaline phosphatase (ALP) [ 12 ]. ALP is a dimeric metalloenzyme with a molecular weight of about 115–165 kDa [ 37 , 38 ], where two identical subunits of about 56 kDa act to catalyse the hydrolysis of phosphate monoester and diester bonds in alkaline environments [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

An investigation of alkaline phosphatase enzymatic activity after electrospinning and electrospraying

Onyekuru

Moreira²,

Zhang

et al. 2021

Journal of Drug Delivery Science and Technology

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The high target specificity and multifunctionality of proteins has led to great interest in their clinical use. To this end, the development of delivery systems capable of preserving their bioactivity and improving bioavailability is pivotal to achieve high effectiveness and satisfactory therapeutic outcomes. Electrohydrodynamic (EHD) techniques, namely electrospinning and electrospraying, have been widely explored for protein encapsulation and delivery. In this work, monoaxial and coaxial electrospinning and electrospraying were used to encapsulate alkaline phosphatase (ALP) into poly(ethylene oxide) fibres and particles, respectively, and the effects of the processing techniques on the integrity and bioactivity of the enzyme were assessed. A full morphological and physicochemical characterisation of the blend and core-shell products was performed. ALP was successfully encapsulated within monolithic and core-shell electrospun fibres and electrosprayed particles, with drug loadings and encapsulation efficiencies of up to 21% and 99%, respectively. Monoaxial and coaxial electrospinning were equally effective in preserving ALP function, leading to no activity loss compared to fresh aqueous solutions of the enzyme. While the same result was observed for monoaxial electrospraying, coaxial electrospraying of ALP caused a 40% reduction in its bioactivity, which was attributed to the high voltage (22.5 kV) used during processing. This demonstrates that choosing between blend and coaxial EHD processing for protein encapsulation is not always straightforward, being highly dependent on the chosen therapeutic agent and the effects of the processing conditions on its bioactivity.

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Protein encapsulation by electrospinning and electrospraying

Cited by 75 publications

References 198 publications

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing

Fortification of chocolate usingMoringa oleiferaextract encapsulated in microemulsions

An investigation of alkaline phosphatase enzymatic activity after electrospinning and electrospraying

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