Protein corona formation moderates the release kinetics of ion channel antagonists from transferrin-functionalized polymeric nanoparticles

Naidu, Priya S. R.; Denham, Eleanor; Bartlett, Carole A.; McGonigle, Terry; Taylor, Nicolas L.; Nörret, Marck; Smith, Nicole M.; Dunlop, Sarah; Iyer, K. Swaminathan; Fitzgerald, Maria

doi:10.1039/c9ra09523c

Cited by 12 publications

(6 citation statements)

References 88 publications

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“…Furthermore, we used the BCA method to estimate the amount of protein adsorbed on the prepared NPs . Naked barley, PEG-, BSA-, CHI-, and CMs-NPs were used as controls.…”

Section: Results and Discussionmentioning

confidence: 99%

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Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology

Graván,

Peña-Martín,

de Andrés

et al. 2023

ACS Appl. Mater. Interfaces

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Nanotechnological platforms offer advantages over conventional therapeutic and diagnostic modalities. However, the efficient biointerfacing of nanomaterials for biomedical applications remains challenging. In recent years, nanoparticles (NPs) with different coatings have been developed to reduce nonspecific interactions, prolong circulation time, and improve therapeutic outcomes. This study aims to compare various NP coatings to enhance surface engineering for more effective nanomedicines. We prepared and characterized polystyrene NPs with different coatings of poly(ethylene glycol), bovine serum albumin, chitosan, and cell membranes from a human breast cancer cell line. The coating was found to affect the colloidal stability, adhesion, and elastic modulus of NPs. Protein corona formation and cellular uptake of NPs were also investigated, and a 3D tumor model was employed to provide a more realistic representation of the tumor microenvironment. The prepared NPs were found to reduce protein adsorption, and cell-membrane-coated NPs showed significantly higher cellular uptake. The secretion of proinflammatory cytokines in human monocytes after incubation with the prepared NPs was evaluated. Overall, the study demonstrates the importance of coatings in affecting the behavior and interaction of nanosystems with biological entities. The findings provide insight into bionano interactions and are important for the effective implementation of stealth surface engineering designs.

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“…Furthermore, we used the BCA method to estimate the amount of protein adsorbed on the prepared NPs . Naked barley, PEG-, BSA-, CHI-, and CMs-NPs were used as controls.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Furthermore, we used the BCA method to estimate the amount of protein adsorbed on the prepared NPs. 42 Naked barley, PEG-, BSA-, CHI-, and CMs-NPs were used as controls. In comparison to bare NPs, all coated NPs exhibited a significant reduction in the number of adsorbed proteins (Figure 4F).…”

Section: Colloidal Stabilitymentioning

confidence: 99%

Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology

Graván,

Peña-Martín,

de Andrés

et al. 2023

ACS Appl. Mater. Interfaces

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“…The ability of polyplexes to protect nucleic acid cargoes under physiological conditions was evaluated by incubating polyplexes in a physiologically relevant concentration (55% FBS) of serum or DNase I for 30 min or 1 h, respectively, followed by performing gel electrophoresis. ,− Serum incubation revealed partial destabilization of pDNA polyplexes prepared from polyMDET and polyMDET-C10 polyplexes, whereas the other polyplexes remained stable (Figure S9). Figure S10 shows that all polyplexes protected DNA from DNase I degradation.…”

Section: Resultsmentioning

confidence: 99%

Excipient-Free Ionizable Polyester Nanoparticles for Lung-Selective and Innate Immune Cell Plasmid DNA and mRNA Transfection

Chakraborty

Dharmaraj

Truong

et al. 2022

ACS Appl. Mater. Interfaces

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Extrahepatic nucleic acid delivery using polymers typically requires the synthesis and purification of custom monomers, post-synthetic modifications, and incorporation of additional excipients to augment their stability, endosomal escape, and in vivo effectiveness. Here, we report the development of a single-component and excipient-free, polyester-based nucleic acid delivery nanoparticle platform comprising ionizable N-methyldiethanolamine (MDET) and various hydrophobic alkyl diols (Cp) that achieves lung-selective nucleic acid transfection in vivo. PolyMDET and polyMDET-Cp polyplexes displayed high serum and enzymatic stability, while delivering pDNA or mRNA to "hard-to-transfect" innate immune cells. PolyMDET-C4 and polyMDET-C6 mediated high protein expression in lung alveolar macrophages and dendritic cells without inducing tissue damage or systemic inflammatory responses. Improved strategies using readily available starting materials to produce a simple, excipient-free, non-viral nucleic acid delivery platform with lung-selective and innate immune cell tropism has the potential to expedite clinical deployment of polymer-based genetic medicines.

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“…They concluded on the difficulty to compare in vitro and in vivo analyses and the unpredictability of PC in vivo because it results from a dynamic process. Since then, therapeutic NPs have been extensively explored 120 but few papers are focusing on the studies of the in vivo interactions of nanohybrids with proteins (less than 20 at the date of the writing).…”

Section: Studying Pc In Vivo : the Way To Improve ...mentioning

confidence: 99%

In vivo protein corona on nanoparticles: does the control of all material parameters orient the biological behavior?

et al. 2021

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Protein corona formation moderates the release kinetics of ion channel antagonists from transferrin-functionalized polymeric nanoparticles

Abstract: Transferrin (Tf)-functionalized p(HEMA-ran-GMA) nanoparticles were designed to incorporate and release a water-soluble combination of three ion channel antagonists, identified as a promising therapy for secondary degeneration following neurotrauma.

Cited by 12 publications

References 88 publications

Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology

Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology

Excipient-Free Ionizable Polyester Nanoparticles for Lung-Selective and Innate Immune Cell Plasmid DNA and mRNA Transfection

In vivo protein corona on nanoparticles: does the control of all material parameters orient the biological behavior?

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