Protein compounds of Bacillus subtilis with in vitro antifungal activity against Pseudocercospora fijiensis (Morelet)

Cruz-Martín, Mileidy; Mena, Eilyn; Acosta-Suárez, Mayra; Pichardo, Tatiana; Rodríguez, Eloisa; Alvarado-Capó, Yelenys

doi:10.1007/s42770-019-00136-9

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Single-factor experiments were conducted to preliminarily determine the effect of initial pH, inoculum volume, and rotation speed on the antifungal effect. In the experiment, set different values for each factor: initial pH (5,7,9), inoculum volume (1%, 2%, 3%) and rotation speed (160 rpm, 180 rpm, 200 rpm).…”

Section: Single-factor Experimentsmentioning

confidence: 99%

Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

Chen

2023

Environ Sci Pollut Res

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Apple anthracnose is a fruit fungal disease that is currently recognized as one of the most serious threats to apples worldwide. In this study, antifungal protein from B. subtilis SL-44 was isolated, puri ed, identi ed, and applied for apple anthracnose control. The antagonistic experiment showed that SL-44 had an excellent broad spectrum against plant pathogenic fungi. Initial pH, inoculum volume, and rotation speed were evaluated by single-factor experiments and Box-Behnken Design and which were optimized to be 7, 2%, and 180 rpm, respectively. Under these conditions, the yield of antifungal protein increased by 45.83% compared to that before optimization. The crude protein from SL-44 was isolated and puri ed by (NH 4 ) 2 SO 4 precipitation, DEAE-Sepharose fast ow column, and Sephadex G-100 column.TEM revealed that antifungal protein could severely disrupt the mycelial of C. gloeosporioides, resulting in the loss of its intracellular contents. LC-MS analyzed that antifungal protein was likely to be an uncharacterized protein YefB with a molecular weight of 35 kDa. Additionally, antifungal protein signi cantly alleviated the size of the lesion to more than 70% in the apple infection protection test. In conclusion, B. subtilis SL-44 and its main antimicrobial components have remarkable potential in the development of fungicides for biological control of apple anthracnose.

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Section: Single-factor Experimentsmentioning

confidence: 99%

Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

Chen

2023

Environ Sci Pollut Res

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Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

Tuyến¹,

Trung²,

Thảo³

et al. 2023

International Biodeterioration & Biodegradation

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Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

Chen

2022

Preprint

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Apple anthracnose is a fruit fungal disease that is currently recognized as one of the most serious threats to apples worldwide. In this study, antifungal protein from B. subtilis SL-44 was isolated, purified, identified, and applied for apple anthracnose control. The antagonistic experiment showed that SL-44 had an excellent broad spectrum against plant pathogenic fungi. Initial pH, inoculum volume, and rotation speed were evaluated by single-factor experiments and Box-Behnken Design and which were optimized to be 7, 2%, and 180 rpm, respectively. Under these conditions, the yield of antifungal protein increased by 45.83% compared to that before optimization. The crude protein from SL-44 was isolated and purified by (NH4)2SO4 precipitation, DEAE-Sepharose fast flow column, and Sephadex G-100 column. TEM revealed that antifungal protein could severely disrupt the mycelial of C. gloeosporioides, resulting in the loss of its intracellular contents. LC-MS analyzed that antifungal protein was likely to be an uncharacterized protein YefB with a molecular weight of 35 kDa. Additionally, antifungal protein significantly alleviated the size of the lesion to more than 70% in the apple infection protection test. In conclusion, B. subtilis SL-44 and its main antimicrobial components have remarkable potential in the development of fungicides for biological control of apple anthracnose.

show abstract

Protein compounds of Bacillus subtilis with in vitro antifungal activity against Pseudocercospora fijiensis (Morelet)

Cited by 3 publications

References 13 publications

Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple

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