Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation

Araújo, Tamara Teodoro; Carvalho, Thamyris de Souza; Dionízio, Aline; Debortolli, Ana Luiza Bogaz; Ventura, Talita Mendes Oliveira; Souza, Beatriz Martines de; Feitosa, Chelsea Maria Vilas Boas; Barbosa, Heloisa Aparecida Pereira; Ribeiro, Caroline Fernanda da Silva; Martini, Tatiana; Taira, Éven Akemi; Shibao, Priscila Yumi Tanaka; Henrique-Silva, Flávio; Marchetto, Reinaldo; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.jdent.2021.103874

Cited by 15 publications

(16 citation statements)

References 39 publications

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“…It has been reported that statherin‐derived peptides with 15 N‐terminal residues or more confer protection [38]. The peptide StatpSpS containing the 15 N‐terminal residues of statherin, with serines 2 and 3 phosphorylated, was shown to reduce initial enamel erosion in vitro [39] and in vivo [22], because after a mouth rinse with this peptide there is an increase in acid‐resistant proteins in the acquired enamel pellicle [22, 28]. It is also interesting to note that proteins such as alpha‐amylase 1 and alpha‐amylase 2B, related to carbohydrate metabolism [40], were found in higher amounts in the caries‐free group.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Oliveira

Buzalaf

Silva

et al. 2023

European J Oral Sciences

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Acquired enamel pellicle plays an important role in the pathogenesis of early childhood caries (ECC), working as a protective interface between the tooth and the oral cavity. The aim of this cross-sectional in vivo proteomic study was to compare the acquired enamel pellicle protein profile of 3-5-year-old children with ECC (n = 10) and caries-free children (n = 10). Acquired enamel pellicle samples were collected and processed for proteomic analysis (nLC-ESI-MS/MS). In total, 241 proteins were identified. Basic salivary proline-rich protein 1 and 2, Cystatin-B, and SA were found only in the caries free group. When comparing caries free and ECC groups, lower protein levels were found in the caries free group for hemoglobin subunit beta, delta, epsilon, gamma-2, globin domain-containing protein and gamma-1, neutrophil defensin 3, serum albumin, protein S100-A8, and S100-A9. The proteins histatin-1, statherin, salivary acidic proline-rich phosphoprotein ½, proline-rich protein 4, submaxillary gland androgen-regulated protein 3B, alpha-amylase 1 and 2B were found at higher levels in the caries free group. The exclusive and the proteins found at higher levels in the caries free group might have protective functions that play a role in the prevention of caries, besides providing important insights to be evaluated in future studies for the possible development of new therapeutic strategies for ECC.

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Section: Discussionmentioning

confidence: 99%

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Oliveira

Buzalaf

Silva

et al. 2023

European J Oral Sciences

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“…These complexes serve as protection against proteolytic processes and lead to a distribution of these proteins on all surfaces of the oral cavity [ 160 , 161 ]. On this basis, current research tries to focus on identifying specific proteins and modifying their sequences, hoping that these new designed proteins are more efficient than evolutionarily developed physiological proteins of the oral cavity [ 140 , 141 , 160 , 162 ]. Currently, the investigations and development of such proteins are still at an early stage.…”

Section: Impact Of Proteins On De- and Remineralizationmentioning

confidence: 99%

“…At the moment, bioinspired pellicle engineering is being investigated by several study groups worldwide [ 140 , 141 , 160 , 162 ].…”

Section: Promotion Of Remineralizationmentioning

confidence: 99%

Caries Management—The Role of Surface Interactions in De- and Remineralization-Processes

Flemming

Hannig

2022

JCM

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Background: Bioadhesion and surface interactions on enamel are of essential relevance for initiation, progression and prevention of caries and erosions. Salivary proteins on and within initial carious and erosive lesions can facilitate or aggravate de- and remineralization. This applies for the pellicle layer, the subsurface pellicle and for proteins within initial carious lesions. Little is known about these proteinaceous structures related to initial caries and erosion. Accordingly, there is a considerable demand for an understanding of the underlying processes occurring at the interface between the tooth surface and the oral cavity in order to develop novel agents that limit and modulate caries and erosion. Objectives and findings: The present paper depicts the current knowledge of the processes occurring at the interface of the tooth surface and the oral fluids. Proteinaceous layers on dental hard tissues can prevent or aggravate demineralization processes, whereas proteins within initial erosive or carious lesions might hinder remineralization considerably and restrict the entry of ions into lesions. Conclusions: Despite the fact that organic–inorganic surface interactions are of essential relevance for de- and remineralization processes at the tooth surface, there is limited knowledge on these clinically relevant phenomena. Accordingly, intensive research is necessary to develop new approaches in preventive dentistry.

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“…A statherin-derived peptide containing 15 N-terminal residues with serines 2 and 3 phosphorylated (Stn15pSpS) has been employed in acquired pellicle engineering. Rinsing with this peptide increases acid-resistant proteins within the acquired pellicle [Carvalho et al, 2020;Taira et al, 2021;Araújo et al, 2022] and protects against initial intrinsic erosion [Carvalho et al, 2020;Taira et al, 2020]. Moreover, in patients suffering diet-induced dental erosion, the concentration of statherin in the acquired enamel pellicle was 35% lower when compared to healthy age-matched controls [Carpenter et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that most of the protection is conferred by the proteins found in the basal layer of the acquired pellicle, which are not displaced even after severe erosive challenges [Hannig 1999]. Thus, the reinforcement of the basal layer of the acquired pellicle with acid-resistant proteins has been suggested as a possible new strategy to protect against ETW [Carvalho et al, 2020;Pelá et al, 2021a;Araújo et al, 2022], which has been named "acquired pellicle engineering. "…”

Section: Introductionmentioning

confidence: 99%

Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro

et al. 2023

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The effect of solutions containing a statherin-derived peptide (Stn15pSpS) on the protection against enamel erosion in vitro was evaluated. Bovine enamel specimens were divided into 4 groups (n = 15/group): 1) Deionized water (negative control), 2) Elmex Erosion Protection™ (positive control), 3) 1.88 × 10-5 M Stn15pSpS and 4) 3.76 × 10-5 M Stn15pSpS. The solutions were applied on the specimens for 1 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH-cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The solutions were applied again during pH cycling, 2 times/day for 1 min after the first and last erosive challenges. Enamel loss (µm) was assessed by contact profilometry. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). The best protection against erosion was conferred by Elmex Erosion Protection that significantly differed from all the other treatments, followed by the solutions containing Stn15pSpS, regardless of the concentration. However, 3.76 × 10-5 M Stn15pSpS did not differ from the negative control. The solution containing the lower concentration of Stn15pSpS protected against erosion in vitro, which should be confirmed using protocols that more closely resemble the clinical condition.

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Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation

Cited by 15 publications

References 39 publications

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Caries Management—The Role of Surface Interactions in De- and Remineralization-Processes

Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro

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