Protein and peptide analysis by capillary zone electrophoresis and micellar electrokinetic chromatography

Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis

“…Furthermore, both the interactions between biopolymers and the additives in buffer solutions [5,[28][29][30][31] and adsorption of biopolymers on the capillary wall [29,32,34] can greatly affect separation selectivity. Due to the possible lysis of microorganisms by ionic surfactants [28,30,31,34] their applicability remains only for the separation of proteins in the bioanalytical methods.…”

Influence of buffer composition and sample pretreatment on efficiency separation for monitoring neuropeptides in plasma using capillary electrophoresis

“…Standard calibration curves were obtained for each of the identified neuropeptides in order to do a quantitative analysis of these peptides in human plasma. The curves were constructed using neuropeptide standards at concentration ranges from 50 to 200 ng/mL and normalizing the corresponding peak area to the area of internal standard, a fragment of dynorphin (1)(2)(3)(4)(5)(6)(7)(8)(9). Good linear relationships were obtained over the whole range of concentrations, such as correlation coefficients which were higher than 0.9965.…”

“…Commonly used methods of separations in this area are sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), size-exclusion chromatography (SEC) [4], radioimmunoassay (RIA) and high-performance liquid chromatography (HPLC). Recently, the capillary electrophoresis (CE) method has been used in analyzing proteins, peptides, and drugs for its high resolution, high efficiency, small sample size, and not having to handle bio-hazardous radioactive materials [5].…”

Separation of phenotypically indistinguishable Candida species, C. orthopsilosis, C. metapsilosis and C. parapsilosis, by capillary electromigration techniques

“…We used experiences from previous works [25,29,38,39] where the reproducible results and the smooth pH gradient in the narrow pH range were achieved by CIEF using segmented injection. Some difficulties such as the adsorption of microorganisms onto the capillary wall [29,[40][41][42] were solved based upon different surface properties of the cells during CE separation, as achieved by using appropriate buffer solutions additives [29,40,[43][44][45][46], e.g. poly(ethylene glycol) (PEG) [ …”