Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size

Katiyar, Amit; Ji, Lei; Smirniotis, Panagiotis G.; Pinto, Neville G.

doi:10.1016/j.chroma.2004.10.077

Cited by 169 publications

(142 citation statements)

References 28 publications

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“…Thus, SBA-15/PAni isotherm maintained the typical SBA-15 isotherm shape, but with the PAni contents. In addition, the data reported in Table 2 indicate a decrease in specific surface area, and mesopore volume, and pore size of SBA15/PAni was consistent with the grafting of PAni species on the silica surface at the molecular level, which showed that the polymer well incorporated into the channels of SBA-15 (41,(44)(45)(46).…”

Section: Characterization Analysesmentioning

confidence: 52%

Investigation of Acid Blue 62 Dye Adsorption using SBA-15/Polyaniline Mesoporous Nanocomposite: Kinetic and Thermodynamic Study

et al. 2017

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Background and Purpose: This study aimed to investigate the adsorption of Acid Blue 62 (AB62) as an anionic dye from aqueous solution onto as SBA-15/Polyaniline (SBA-15/PAni) mesoporous nanocomposite a low-cost adsorbent and feasible. Materials and Methods: Fourier transform infra-red spectroscopy (FTIR), Filed Emission Scanning Electron Microscope (FESEM), Transmission Electron Microscope (TEM), X-ray Diffraction (XRD), and BET were used to examine the structural characteristics of obtained adsorbent. The effective parameters on batch adsorption process such as pH, dosage, and time were investigated and optimized. For determining the type of kinetic model, pseudo first order, pseudo second order, Elovich and intra-particle diffusion kinetic models were applied. Thermodynamic parameters such as changes in Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) were calculated. Results: Maximum BET specific surface and pore volume on the adsorbent were 224.4 m 2 /g and 0.46 cm 3 /g, respectively. The obtained optimized condition was as follows: pH=2, time=60 min, temperature 25° C, and adsorbent dose = 0.3 g/l. The adsorption kinetic data well-fitted by pseudo-second order kinetic equation. The negative values of ΔG° and ΔH° (-4.012 KJ/mol) and the positive value of ΔS° (0.409 J/mol K) showed that the AB62 adsorption process was spontaneous, physi-sorption, feasible and exothermic. Conclusion: SBA-15/PAni can well be used as a low-cost surface adsorbent for removal of AB62 from aqueous media.

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Section: Characterization Analysesmentioning

confidence: 52%

Investigation of Acid Blue 62 Dye Adsorption using SBA-15/Polyaniline Mesoporous Nanocomposite: Kinetic and Thermodynamic Study

et al. 2017

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“…For example, myoglobin, cytochrome c (cyt c) and (BSA) have been shown to have a higher adsorption capacity onto the materials at a pH equal or less than the pI point of the protein. [88][89][90][91] This is caused by the electrostatic interactions between the protein and the MSNs. Because the repulsive forces between the adsorbed protein on the surface of the material and protein left in solution are minimal at its pI point.…”

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confidence: 99%

Mesoporous silica nanoparticles for bioadsorption, enzyme immobilisation, and delivery carriers

et al. 2011

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Mesoporous silica nanoparticles (MSNs) provide a non-invasive and biocompatible delivery platform for a broad range of applications in therapeutics, pharmaceuticals and diagnosis. The creation of smart, stimuli-responsive systems that respond to subtle changes in the local cellular environment are likely to yield long term solutions to many of the current drug/gene/DNA/RNA delivery problems. In addition, MSNs have proven to be promising supports for enzyme immobilisation, enabling the enzymes to retain their activity, affording them greater potential for wide applications in biocatalysis and energy. This review provides a comprehensive summary of the advances made in the last decade and a future outlook on possible applications of MSNs as nanocontainers for storage and delivery of biomolecules. We discuss some of the important factors affecting the adsorption and release of biomolecules in MSNs and review of the cytotoxicity aspects of such nanomaterials. The review also highlights some promising work on enzyme immobilisation using mesoporous silica nanoparticles.

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“…Proteins with the hydrodynamic diameter that is smaller than the pore diameter can easily enter the mesopores and show higher loading capacity, while the ones with larger diameter adsorb on the outer surface of the nanoparticles. [45] Previously, we reported the use of HMSA surface functionalised with amino groups for the development of BVDV-1 E2 nanovaccine. [24] However, the amount of protein that could be loaded on the HMSA, which was only 80 !g of the oE2 protein per mg of HMSA (particle size 120 nm).…”

Section: Discussionmentioning

confidence: 99%

“…[44] Different organic functional groups such as amino (-NH 2 ), thiol (-SH), vinyl (-CH=CH 2 ) and phenyl (-C 6 H 5 ) can be incorporated into or onto the walls of the silica particles. [45] The advantage of functionalisation of silica depends on the type of application. For example, amino functionalised have been explored in areas such as adsorption [46], catalysis [47] and enzyme immobilisation [48] with very promising results.…”

Section: Silica Nanoparticles Can Be the New Generation Of Adjuvants mentioning

confidence: 99%

“…[30] In addition many research studies have focussed on synthesis, biocompatibility and biodistribution of MSNs. [15,[31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] Various studies have shown that MSNs are excellent vehicles for gene and drug delivery because of their ease of synthesis, surface functionalisation, excellent in vivo biocompatibility as well as good thermal and chemical stability. [40,46,47] Additionally, MSNs are capable of controlling the release of drugs or proteins depending on their size, shape and surface modification.…”

Section: Adjuvants For Vaccine Deliverymentioning

confidence: 99%

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Development of nanoparticle based vaccine delivery systems

Mody¹

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Development of subunit vaccine formulation requires a careful selection of potent antigen, efficient adjuvant and route of delivery. The desirable physicochemical characteristics of the mesoporous silica nanoparticles (MSNs) such as ease of synthesis, excellent in vivo biocompatibility and good thermal and chemical stability, make them optimal nanocarriers for various biomolecules (Mody et al. Nanoscale, 2013). Freeze-drying process can be used to further improve both th e short and long-term stability of protein-loaded nanovaccine components (Mody et al. Drug Deliv. Lett., 2012). Bovine Viral Diarrhoea Virus-1 (BVDV-1) is one of the most serious pathogens, which causes tremendous economic loss to the cattle industry worldwide, meriting the development of improved subunit vaccines. E2 is the structural envelope glycoprotein of BVDV-1 and is a major immunogenic determinant, making it an ideal candidate for the development of subunit vaccines. ) was a major limitation of this study. Therefore, HMSAs, (particle size 120 nm, pores on the wall of entrance sized 2 nm) were investigated for developing recombinant BVDV-1 E2 nanovaccine (60-80 µg oE2 /mg HMSA). The immunogenicity of the oE2/HMSA nanovaccine before and after a freeze-drying with trehalose (5%) and glycine (1%) was evaluated in a sheep trial. The non-FD and FD oE2/HMSA generated oE2 specific antibody and cell-mediated immune responses after three subcutaneous injections with 500 !g oE2 adsorbed to 6.2 mg HMSA.Importantly, it was found that the long-term cell-mediated immune responses were detectable up to five months after immunisation (Manuscript submitted to PLoS ONE).iv

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Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size

Cited by 169 publications

References 28 publications

Investigation of Acid Blue 62 Dye Adsorption using SBA-15/Polyaniline Mesoporous Nanocomposite: Kinetic and Thermodynamic Study

Investigation of Acid Blue 62 Dye Adsorption using SBA-15/Polyaniline Mesoporous Nanocomposite: Kinetic and Thermodynamic Study

Mesoporous silica nanoparticles for bioadsorption, enzyme immobilisation, and delivery carriers

Development of nanoparticle based vaccine delivery systems

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