2008
DOI: 10.1088/0953-8984/20/40/404226
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Protein adsorption on tailored substrates: long-range forces and conformational changes

Abstract: Adsorption of proteins onto solid surfaces is an everyday phenomenon that is not yet fully understood. To further the current understanding, we have performed in situ ellipsometry studies to reveal the adsorption kinetics of three different proteins, lysozyme, α-amylase and bovine serum albumin. As substrates we offer Si wafers with a controlled Si oxide layer thickness and a hydrophilic or hydrophobic surface functionalization, allowing the tailoring of the influence of short- and long-range interactions. Our… Show more

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Cited by 55 publications
(82 citation statements)
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References 43 publications
(61 reference statements)
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“…This result is consistent with what has been observed in the experiments from single protein absorption when conformational changes occur 36 . Moreover, our analysis for competitive adsorption shows, as for T = 300 K, that the linear regime of Fib adsorption coincides with the end of the desorption of the Alb and the beginning of desorption of IgG.…”
Section: Effect Of Energy Depletionsupporting
confidence: 93%
“…This result is consistent with what has been observed in the experiments from single protein absorption when conformational changes occur 36 . Moreover, our analysis for competitive adsorption shows, as for T = 300 K, that the linear regime of Fib adsorption coincides with the end of the desorption of the Alb and the beginning of desorption of IgG.…”
Section: Effect Of Energy Depletionsupporting
confidence: 93%
“…41,42 Based on Monte-Carlo simulations featuring surface processes such as surface mobility and conformational changes, the distinctions were invoked by the influence of the vdW-interactions on the time scale of these processes. 43 X-ray reflectivity experiments corroborated these findings. 44 Recent studies discovered that the influence of the subsurface material on adhesion is sensed by larger biological objects, too: The unspecific adhesion of bacteria from the Staphylococcus genus is affected by the properties of the subsurface material, as could be shown by AFM force spectroscopy measurements.…”
Section: Interactions In Biological Systemssupporting
confidence: 56%
“…Recently, a similar influence was detected on the adsorption kinetics of proteins [11][12][13] and the adhesion of bacteria [14]. These experiments used materials whose contribution to the potential had been tuned by means of surface stratification.…”
Section: Introductionmentioning
confidence: 66%