Protective efficacy of monoclonal antibodies to class 1 and class 3 outer membrane proteins of Neisseria meningitidis B:15:P1.16 in infant rat infection model: new prospects for vaccine development

Meningococcal infection: --a review based upon papers presented at the 155th Meeting of the Pathological Society of Great Britain and Ireland at Southampton; 8 July, 1987

“…Antibodies against the class-2/3 OMP are only weakly bactericidal, particularly when the test bacteria are grown to stationary phase or in conditions of glucose limitation . These results were confirmed by testing the protective capacity of these monoclonal antibodies in an infant rat meningitis model (Saukkonen et al, 1987). The current vaccine trials (Fraholm et al, 1986) with these first generation outer-membrane-protein vaccines will eventually allow the identification of the epitopes capable of inducing bactericidal, protective antibodies in man.…”

“…Subtyping is according to the reactions with OMP 1 (Frasch et al, 1985a). Since the latter OMP has been shown to be related to the induction of protective responses in experimental animals (Saukkonen et al, 1987) and since antibodies to this OMP are bactericidal (Mocca et al, 1984;Poolman et al, 1985), it is the most likely candidate antigen for a vaccine.…”

N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil

“…The class 1 protein is unique to meningococci, it is immunogenic and antibodies directed against it are bactericidal in vitro ) and protect against infection in an experimental animal model (Saukkonen et al 1987).…”

Identification of bacterial genes required for in-vivo survival

“…The mutants were then screened for their ability to cause systemic infection in the neonatal rat model. This model has previously been used to study the influence of lipopolysaccharide, capsule and iron acquisition on pathogenesis (Stojiljkovic et al 1995 ;Vogel et al 1996), and the protective efficacy of antibodies against outer membrane proteins (Saukkonen et al 1987). STM of N. meningitidis led to the identification of 73 genes required for bacteraemic disease (Sun et al 2000).…”