Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats

Özkol, Halil; Bulut, Gülay; Balahoroğlu, Ragıp; Tülüce, Yasin; Özkol, Hatice Uce

doi:10.1007/s12011-016-0762-8

Cited by 30 publications

(22 citation statements)

References 52 publications

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“…The most common medications for ALD are classified into three categories: supplemental raw materials for liver cell metabolism [12], opioid receptor antagonists [12], and agents that manage and improve alcohol metabolism [13, 14]. However, drug dependence, vomiting, dermatitis, dizziness, and leukopenia have been observed in ALD patients after long-term treatment with these agents [15].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Liu

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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In the present study, the components of A. cinnamomea (AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use of A. cinnamomea as a functional food and/or medicine.

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Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Liu

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Ozkol et al in their experimental model have shown that supplementation of selenium (alone or in combination with vitamin E and N-acetylcysteine) exerts hepatoprotective effects in rats exposed to ethanol [20]. The authors have demonstrated that the positive effects of selenium are likely to result from its antioxidative and neutralising action against free radicals.…”

Section: Discussionmentioning

confidence: 99%

Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis

Prystupa¹,

Kiciński²,

Luchowska-Kocot³

et al. 2017

Ann Agric Environ Med.

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Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines in patients with alcoholic liver cirrhosis. Ann Agric Env Med. 2017; 24(3): 544-548. doi: 10.26444/aaem/76937 Abstract Introduction and objective. Liver cirrhosis is a disease involving the liver parenchyma, which is characterised by fibrosis. and impaired architectonics of the parenchyma with regenerative nodules. The aim of the study was to determine the relationship between stage of alcoholic liver cirrhosis, concentrations of selenium, zinc and profibrotic and proangiogenic cytokines ENG). Materials and method. The study included 99 patients with alcoholic cirrhosis and 20 healthy subjects. Ion chromatography with UV/VIS detection was used for determination of zinc ions in the previously mineralized serum samples. The measurements of selenium were performed with the ContrAA700 high-resolution continuum source graphite tube atomic absorption spectrometer. ELISA was used to determine concentration of FGF-19 and ENG in serum samples. Results. Concentrations of zinc and selenium were significantly decreased in cirrhotic patients (p<0.001 for both). The highest concentration of FGF-19 was found in Child-Pugh stage C liver cirrhosis patients (806.9±650.3 pg/ml), and was significantly higher than observed in controls (p=0.005) and stage A patients (compensated cirrhosis) (p=0.02). The highest concentration of ENG was demonstrated in the control group (3.24±148 ng/ml) while the lowest in patients with decompensated cirrhosis (7.32±5.39 ng/ml and 7.92±4.18 ng/ml for stage B and C; p=0.03 and p=0.02, respectively). The use of the multiple-variable model demonstrated that the independent factors affecting the concentration of ENG were the concentration of bilirubin (p=0.02), INR (p=0.01) and duration of alcohol abuse (p=0.02). The independent determinants of FGF-19 concentrations were found to be the stage (severity) of liver cirrhosis (p=0.04) and INR (p=0.03). Conclusions. Concentrations of zinc and selenium in serum of patients with alcoholic liver cirrhosis are not independently related to concentrations of FGF-19 and ENG.

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“…A recent metaanalysis showed that vitamin E significantly improved liver function and histologic changes in patients with hepatic steatosis [22]. Ozkol et all showed relative protection against acute EtOH toxicity, in both single and combined pretreatments of Selenium, N-Acetylcysteine, and Vit E supplementation were seen in brain, liver, and kidney tissues, probably by antioxidant mechanism [23].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E

Jordão¹,

Monteiro²,

Santos³

et al. 2017

AJBLS

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Abstract:The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.

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Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats

Cited by 30 publications

References 52 publications

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis

Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E

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