Protective effects of salep against isoniazid liver toxicity in wistar rats

Jahromi, Hossein Kargar; Pourahmad, Morteza; Abedi, Heidar Ali; karimi, Mohadeseh; Jahromi, Zahra Kargar

doi:10.1016/j.jtcme.2017.06.001

Cited by 16 publications

(4 citation statements)

References 27 publications

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“…Histological examination of liver sections in high methionine rats showed marked alterations in the liver tissue, as inflammatory cells infiltration, ballooned hepathocytes, necrosis and sinusoid hemorrhage. These observations are similar to others 27 , 28 . Furthermore, altered liver structure confirmed the release of hepatic proteins in plasma as outlined above.…”

Section: Discussionsupporting

confidence: 93%

High methionine diet mediated oxidative stress and proteasome impairment causes toxicity in liver

Derouiche,

Djemil,

Sebihi

et al. 2024

Sci Rep

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Methionine (Met) rich diet inducing oxidative stress is reported to alter many organs. Proteasome as a regulator of oxidative stress can be targeted. This study was performed to investigate if excessive methionine supplementation causes hepatotoxicity related to proteasome dysfunction under endogenous oxidative stress in rats. Male Wistar albino rats (n = 16) were divided into controls and treated groups. The treated rats (n = 08) received orally l-methionine (1 g/kg/day) for 21 days. Total homocysteine (tHcy), total oxidant status (TOS), total antioxidant status (TAS), hepatic enzymes levels: aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), with total bilirubin (TBil), albumin (Alb), and C-reactive protein (CRP) were determined in plasma by biochemical assays. Liver supernatants were used for malondialdehyde (MDA), protein carbonyls (PC), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), 20S proteasome activities and their subunits expression, tumor necrosis factor-α (TNF-α), and interleukin 6 (IL-6) evaluation by appropriate methods and light microscopy for liver histological examination. Methionine treatment increased homocysteine, TOS, oxidative stress index (OSI), MDA and PC but decreased TAS, GSH, CAT, SOD, GPx with the 20S proteasome activities and their β subunits expression. Liver proteins: AST, ALT, LDH, ALP, TBil and CRP were increased but Alb was decreased. Liver histology was also altered. An increase in liver TNF-α and IL-6 levels were observed. These findings indicated that methionine supplementation associated oxidative stress and proteasome dysfunction, caused hepatotoxicity and inflammation in rat. Further investigations should be to better understand the relation between methionine, oxidative stress, proteasome, and liver injuries.

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Section: Discussionsupporting

confidence: 93%

High methionine diet mediated oxidative stress and proteasome impairment causes toxicity in liver

Derouiche,

Djemil,

Sebihi

et al. 2024

Sci Rep

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“…Antioxidant and anti-inflammatory impacts of BSE have been reported in several studies (18,19). Kushwah et al (20) have reported decreased serum liver-specific enzymes and malondialdehyde and increased antioxidant enzymes in the liver following BSE treatment.…”

Section: Discussionmentioning

confidence: 97%

Investigating the Possible Ameliorating Impact of Black Seed (Nigella sativa) Hydroethanolic Extract on Liver and Brain Tissue in a Rat Model of Polycystic Ovary

Abdollahi,

Mirghazanfari,

Mehri

2024

Ann Mil Health Sci Res

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Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder in women of reproductive age, which may affect various organs, including the brain and liver. Objectives: This study aimed to investigate the potential ameliorating effects of black seed (Nigella sativa) hydroethanolic extract on the liver and brain tissues in a rat model of polycystic ovary. Methods: In this experimental study, eighteen female rats were divided into three groups: control (PCO rats on day 0 after induction of PCO), vehicle (PCO rats receiving saline via gavage for 28 days), and black seed extract (BSE) (PCO rats receiving BSE via gavage for 28 days). Polycystic ovary was induced by a single intraperitoneal injection of 2 mg/kg estradiol valerate. Liver tissue was evaluated for the average number of damaged hepatocytes (segmented nucleus and cytoplasm shrinkage), number of Kupffer cells, and number of lipid droplets, while brain tissue was assessed for vacuolization, apoptosis, and cell shrinkage. Results: In the BSE group, liver weight significantly decreased, while brain weight increased compared to the vehicle and control groups. Moreover, there was a significant decrease in the number of damaged hepatocytes and Kupffer cells in the BSE group compared to the vehicle group in the liver tissue. In the brain tissue, there was a significant reduction in the number of vacuolations in the BSE group compared to the vehicle group. Conclusions: Black seed extract demonstrated potential ameliorating effects on the adverse impacts of PCO in the liver (reducing damaged hepatocytes and Kupffer cells) and brain (reducing vacuolations). Further histologic and molecular analyses are needed to investigate the protective effects of BSE thoroughly.

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“…The environment dark and light cycle and the humidity were at standard rate, 12/12 hr and 50-55%, respectively. According to previous articles, the prescribed concentration was 40, 80, 160, and 320 mg/kg of body weight (19,20). Therefore, the control and experimental groups in this study include healthy control groups 1 and 2: without receiving any substance and under normal conditions for 60 and 89 days, respectively.…”

Section: Test Designmentioning

confidence: 99%

The effect of aqueous extract of orchid root on the structure of ovary and hypothalamicpituitary- gonadal hormones in polycystic ovary syndrome rat model: An experimental study

Abedi,

Zarrin-Mehr,

Ebrahimi

et al. 2024

IJRM

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Background: Some medical conditions, including polycystic ovarian syndrome (PCOs), may lead to infertility. In PCOs, hormonal imbalance is significant. Antioxidants such as natural antioxidants have many health benefits, including positive effects on hormone production. Objective: Since herbal medicines are more acceptable to people, the present study was designed to evaluate the effect of an aqueous extract of orchid (SA), with antioxidative effects, on the structure of the ovary and the hypothalamic-pituitarygonadal axis hormones and free testosterone in PCOs rats. Materials and Methods: In this experimental study, 64 healthy female Wistar rats (180–200 g) were randomly divided into 60 and 89 day control groups, PCOs, and 4 PCOs + SA groups that received 40, 80, 160, and 320 mg/kg of SA. Serum levels of gonadotropin-releasing hormone, estrogen, progesterone, testosterone, follicle-stimulating hormone, and luteinizing hormone were measured. In addition, the ovaries were extracted and examined histologically. Results: The amount of primordial, primary, secondary, and Graafian follicles and serum levels of Follicle-stimulating hormone and progesterone hormones decreased in PCOs groups, while atretic follicles and the serum levels of gonadotropin-releasing hormone, luteinizing hormone, estrogen, and free testosterone were increased. SA at different doses regulated hormonal and histological imbalances caused by PCOs, and 320 mg/kg was the most effective. Conclusion: The aqueous extract of orchids root can have a positive effect on the improvement of polycystic ovary syndrome. This effect can be achieved by regulating the level of sex hormones and correcting follicular abnormalities in the ovarian tissue. Key words: PCOS, Orchid, Ovary, GnRH, LH, FSH.

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Protective effects of salep against isoniazid liver toxicity in wistar rats

Cited by 16 publications

References 27 publications

High methionine diet mediated oxidative stress and proteasome impairment causes toxicity in liver

High methionine diet mediated oxidative stress and proteasome impairment causes toxicity in liver

Investigating the Possible Ameliorating Impact of Black Seed (Nigella sativa) Hydroethanolic Extract on Liver and Brain Tissue in a Rat Model of Polycystic Ovary

The effect of aqueous extract of orchid root on the structure of ovary and hypothalamicpituitary- gonadal hormones in polycystic ovary syndrome rat model: An experimental study

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