Protective Effects of Micronized Fat against Ultraviolet B–Induced Photoaging

Abstract

Background: Autologous fat grafting has long been an essential technique in cosmetic and reconstructive surgery. Here, the authors report the advantages of a new device for preparing micronized fat, and they also investigated the therapeutic potential of micronized fat against ultraviolet B–induced photoaging. Methods: Micronized fat aliquots were prepared through a connector device with trifoliate blades. The histologic structure and viability of the prepared fat samples were evaluated by calcein AM/propidium iodide staining. The levels of growth factor were measured by enzyme-linked immunosorbent assay, and flow cytometry was used to detect the ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction. The authors also evaluated the effects of micronized fat transplantation through immunohistochemistry and Masson trichrome staining in an animal model of photoaging. Results: The micronized fat had a normal histologic structure and viable adipocytes. It had a higher level of hepatocyte growth factor compared with the control group, and its ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction was also higher than in the control fat preparations. Transplantation of micronized fat preparations in the animal model of photoaging produced increased skin neovascularization, epidermal cell proliferation, and dermal collagen density. Conclusions: The authors’ results demonstrated that the novel device produced micronized fat easily, which can condense adipose tissue. This micronized fat was easy to use with smaller cannulas. It mitigated the signs of cutaneous photoaging and was superior to control fat. Contrary to previous reports, normal histologic structures and viable adipocytes were noted in the micronized fat.

Anti-photoaging Effect of Micronized Fat in Ultraviolet B-Induced Human Dermal Fibroblasts

“…Although no ADSC-specific CD marker has been identified, the component ratio in the MF we prepared was tested in our previous study according to the current available studies on surface-specific antigens of ADSCs. 13 This time, we used CD29, CD31, and CD45 to distinguish ADSCs (CD29 + /CD31 − /CD45 − ) among the SVF. 12 The phenotypic pattern for the identification of MSCs requires the expression of CD29 and lack of CD45.…”

“…13 MF has demonstrated its potential as a means of skin rejuvenation, promoting skin neovascularization, epidermal cell proliferation, and dermal collagen density in animal experiments. 13 To certify the clinical feasibility of MF, we used UV-B-induced HDFs as the experimental model to co-culture with the SVF from MF. Afterward, we examine the levels of the photoaging-related factors in the co-cultured system to evaluate the extent of improvement of photoaging by MF.…”

Common methods in mitochondrial research (Review)

“…When cells are incubated with calcein and co 2+ , both enter the cytoplasm; however, calcein is further captured by mitochondria (211,212). calcein that accumulates in the mitochondria exhibits fluorescent staining, whereas calcein remaining in the cytoplasm or released from the mitochondria into the cytoplasm is rapidly quenched by co 2+ (213)(214)(215)(216)(217)(218)(219). Under normal physiological conditions, mPTP opens transiently and calcein that enters the cytoplasm from the mitochondria is rapidly quenched.…”

Safety assessment of a three‐dimensional‐printed autologous omentum patch: An application on the kidneys as a new treatment approach

“…6,7 The omentum is a subcutaneous and visceral adipose tissue that has been used medically for a long time because it contains various biosources for the repair and regeneration of internal organs. 8,9 Furthermore, micronized adipose tissue is emerging as a new paradigm in regenerative medicine because adjusting the size of adipose tissue facilitates the use of biological components in adipose tissue, including growth factors and cells. 10 These tissue engineering treatment sources have therapeutic efficacy by adjusting to repair the microstructure of the surrounding environment and producing paracrine effects of growth factors and cytokines.…”

“…The omentum is a subcutaneous and visceral adipose tissue that has been used medically for a long time because it contains various biosources for the repair and regeneration of internal organs 8,9 . Furthermore, micronized adipose tissue is emerging as a new paradigm in regenerative medicine because adjusting the size of adipose tissue facilitates the use of biological components in adipose tissue, including growth factors and cells 10 .…”