Protective Effects of Lycium barbarum Polysaccharide on  6-OHDA-Induced Apoptosis in PC12 Cells through the  ROS-NO Pathway

Gao, Kai; Liu, Meiyou; Cao, Jinyi; Yao, Minna; Lu, Yang; Li, Jiankang; Zhu, Xihe; Yang, Zhifu; Wen, Aidong

doi:10.3390/molecules20010293

Cited by 62 publications

(32 citation statements)

References 44 publications

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“…The standard incubation conditions were 5% CO 2 , 37 C. The cell damage was induced by OGD for 16 h [19]. Before OGD, LBPs was administered at0, 100, 300 or 500 lg/ mL for 24 h [20]. In this step, glucose-free DMEM was instead of normal DMEM.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Lycium barbarum polysaccharide alleviates oxygen glucose deprivation-induced PC-12 cells damage by up-regulating miR-24

Song

Lin

Zhu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Objective: This research was aimed to detect the functions of Lycium barbarum polysaccharides (LBPs) on oxygen and glucose deprivation (OGD) injury and potential mechanisms at PC-12 cells. Methods: CCK-8, flow cytometry and reactive oxygen species (ROS) assays were used to detect OGD, LBPs and miR-24 effects on cell viability, apoptosis, and oxidative stress. MiR-24 was transfected and texted by transfection and qRT-PCR. Moreover, the related-protein levels of apoptosis, autophagy and pathways were tested by Western blotting. Results: LBPs significantly enhanced cell viability , inhibited cell apoptosis, autophagy and ROS level in OGD injury. In addition, miR-24 expression was declined in OGD-treated cells, while it was elevated when added LBPs. The preventive effects of LBPs on PC-12 cell damage induced by OGD were reversed by down-regulating miR-24. Furthermore, miR-24 inhibitor declined LBPs-induced change in Wnt/b-catenin and JAK1/STAT3 pathways in OGD-injuried cells. Conclusions: LBPs exhibited preventive effects via up-regulating miR-122 and activating Wnt/b-catenin and JAK1/STAT3 pathways in OGD-induced PC-12 cells. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Lycium barbarum polysaccharide alleviates oxygen glucose deprivation-induced PC-12 cells damage by up-regulating miR-24

Song

Lin

Zhu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…LBP has been thought to be a valuable traditional herbal medicine that is widely used according to Chinese pharmacopoeia (Liang et al, 2018). It can provide vitamins, lutein, betacarotene, zeaxanthin and is rich in polysaccharides (Amagase et al, 2009), making it as a promising and effective agent against aging, neuro-degeneration and inflammation Gao K et al 2015;Li XM, 2007). In the nervous system, LBPs has been proved to repair the neuronal loss, which is attributed to β-amyloid peptide Yu MS et al, 2007)or glutamate excitotoxicity (Ho YS et al, 2009), etc.…”

Section: Discussionmentioning

confidence: 99%

Lycium Barbarum Polysaccharide Induce the Neuronal Differentiation of Mesenchymal Stem Cell for Potential Application in Neural Regeneration

Yan¹,

Xu²,

Teng³

et al. 2019

Neuroquantology

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Lycium barbarum has been considered as a valuable old herbal medicine that has been widely used in Asian country. Lycium barbarum polysaccharides (LBPs), a major component, are proved to possess a variety of beneficial effects including neuro-protection, anticancer properties and anti-aging as well as immune modulation. However, the biological functions and inductive mechanisms of LBP towards the mesenchymal stem cell (MSC) differentiation process have not been fully investigated. In this study, we found that the expression of specific marker of neuron cells including β-tubulin III, NF200 and synapsin was up-regulated when cultured with LBP after 7 days during differentiation of bone marrow-derived MSCs to neuron-like cells, while the pluripotency marker such as Oct4 and Klf4 were down-regulated. By using the staining of phalloidine, the cytoskeleton of the stem cells induced by LBP exhibited lengthening character, resulting in the induction effect of LBP towards BMSCs. Overall, we demonstrate that the directional differentiation process of BMSCs can be partially regulated by LBP. We conclude that this strategy may provide opportunities for physician to improve the effectiveness of stem cell therapy for the treatment of degenerative cerebrovascular disease.

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“…Chemical-induced oxidative stress linked to PD pathogenesis has been widely reported, particularly 6-OHDA, MPTP, paraquat and rotenone (Cantu et al, 2011;Gao et al, 2014;Lu et al, 2015b;Parsanejad et al, 2014). These chemicals mainly impair mitochondrial function such as inhibiting complex I of the electron transport chain, resulting in the production of ROS.…”

Section: Methods Of Identifying Apoptosis or Cell Deathmentioning

confidence: 99%

“…Inhibition of inducible NO synthase (iNOS) by antioxidant flavonoid quercetin prevented 6-OHDA-induced PC12 cell apoptosis and 6-OHDA-stimulated dopaminergic neuronal loss in zebrafish, potentially by downregulating the gene expression of proinflammatory markers such as TNF-alpha, interleukin (IL)-1beta and cyclooxygenase-2 (COX2) (Zhang et al, 2011b). Another antioxidant, Lycium barbarum polysaccharide (LBP), was also able to reduce the accumulation of ROS and nitric oxide levels and to inhibit the expression of NF-κB, iNOS and nNOS in 6-OHDA-exposed PC12 cells, resulting in the prevention of apoptosis (Gao et al, 2014).…”

Section: Supporting Publications 2016: En-955 44mentioning

confidence: 99%

Systematic literature review on Parkinson's disease and Childhood Leukaemia and mode of actions for pesticides

Choi¹,

Polcher²,

Joas³

2016

EFSA Supporting Publications

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This report presents the outcomes of a systematic review (SR) identifying the mechanisms and chemicals involved in the pathogenesis of Parkinson's disease (PD) and childhood leukaemia (CL). This work serves as a basis for defining and mapping the causal linkages between a molecular initiating event (MIE) and a final adverse outcome (AO) that are relevant for the development of a prototype adverse outcome pathway (AOP) for assessing risk factors for PD and CL. Search for literature from 1990 to present was conducted using Web of Science, PubMed and TOXNET, and relevant references were selected using established inclusion/exclusion criteria and ranked using a set of quality control factors.For PD, 7,384 individual references were identified to be relevant for PD pathogenesis and chemicals associated with PD. Dopaminergic neurodegeneration in the substantia nigra leading to locomotor deficits was identified as the AO in PD. Other identified key events in PD pathogenesis include mitochondrial dysfunction, cellular accumulation of alpha-synuclein and oxidative stress. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, rotenone and paraquat are some chemicals identified to be associated with PD pathogenesis.For CL, 1,988 individual references were identified to be relevant for CL pathogenesis and chemicals associated with CL. Chromosomal translocation, genetic damage/mutation and hyperdiploidy are considered as driving forces of CL. Except for infant leukaemia, CL pathogenesis requires a 'two-hit' model with an initiating event occurring in utero followed by a secondary hit potentially occurring after birth. Potential MIEs leading to these driving mechanisms include aberrant DNA topoisomerase II activity and erroneous DNA repair. Epigenetics appears to also play an influential role in CL pathogenesis. Benzene, bioflavonoids and organophosphates are some chemicals identified to be implicated in CL pathogenesis.The challenges of developing AOPs for these two diseases and the data gaps identified from the outcomes of this SR are also elaborated in this report. The present document has been produced and adopted by the bodies identified above as authors. This task has been carried out exclusively by the authors in the context of a contract between the European Food Safety Authority and the authors, awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. KEY WORDSSystematic Review, Parkinson Disease, Childhood Leukaemia, Pathogenesis, Chemicals, Pesticides, Adverse Outcome Pathway SUMMARYThe aim of this project was to perform a systematic review (SR) to collect relevant publications related to the mechanisms involved in the pathogenesis of Parkinson's disease (PD)...

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Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway

Cited by 62 publications

References 44 publications

RETRACTED ARTICLE: Lycium barbarum polysaccharide alleviates oxygen glucose deprivation-induced PC-12 cells damage by up-regulating miR-24

RETRACTED ARTICLE: Lycium barbarum polysaccharide alleviates oxygen glucose deprivation-induced PC-12 cells damage by up-regulating miR-24

Lycium Barbarum Polysaccharide Induce the Neuronal Differentiation of Mesenchymal Stem Cell for Potential Application in Neural Regeneration

Systematic literature review on Parkinson's disease and Childhood Leukaemia and mode of actions for pesticides

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