Protective Effects of Aqueous Extract of &lt;i&gt;Sempervivum tectorum&lt;/i&gt; L (&lt;i&gt;Crassulaceae&lt;/i&gt;) on Aluminium-Induced Oxidative Stress in Rat Blood

Marian, Florin; Alexandra, Tchuidjang; Gabriela, SL; Romeo, CT; Corina, G; Ioan, M; Eugenia, D

doi:10.4314/tjpr.v13i2.2

Cited by 15 publications

(14 citation statements)

References 23 publications

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“…Sempervivum tectorum L., an evergreen plant, known as the common houseleek, is a perennial herb with a dense basal rosette of fleshy sharp-tipped, obovate to oblong leaves having red-colored flowers arranged in terminal cymes [17][18][19]. The houseleek is a drought-resistant plant, native to central and southern Europe, growing on roofs, scree, and weathered rocks, traditionally used fresh rather than dried [18,20].…”

Section: Introductionmentioning

confidence: 99%

“…Aluminum-induced oxidative stress is considered a major cause of male reproductive disorders [7][8][9][10][11]. S. tectorum has been reported to exhibit significant antioxidant activity due to its phytochemical constituents [21]; the antioxidative capacity of S. tectorum can help mitigate the oxidative damage caused by aluminum exposure and protect against reproductive disorders [17].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sempervivum tectorum Extract on Some Biomarkers of Reproductive Function and Levels of Some Trace Elements in Male Rats Exposed to Aluminum

Muselin,

Dumitrescu,

Doma

et al. 2024

Animals

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Aluminum, a contentious trace element found in the environment, has been demonstrated to have harmful effects on both humans and animals. In contrast, Sempervivum tectorum, an evergreen plant, has been found to offer numerous beneficial effects for both humans and animals. Therefore, this study aims to assess the protective effect of S. tectorum on certain reproductive biomarkers in male rats exposed to aluminum. Thirty-five Wistar rats were randomly divided into five groups: NTC (no-treatment control) received distilled water; NC (negative control) received drinking water containing 1 mg/L aluminum sulfate (AS); E1 received 1 mg/L AS along with an 8% S. tectorum extract; PC (positive control) received only 8% S. tectorum extract; E2 received 1 mg/L AS for three months followed by 8% extract for one month. The study analyzed testosterone, LH, FSH, body weight, and the histological structure of the testis, epididymis, and prostate, as well as the levels of zinc, manganese, copper, and iron in these organs. Significant decreases in body weight, testis, and epididymis size were observed in the aluminum-exposed groups compared to the control, whereas these decreases were not significant in the S. tectorum-treated groups compared to the control. Aluminum exposure led to significant decreases in testosterone and LH levels, with FSH levels showing a nonsignificant decrease in males, which were mitigated significantly by the administration of the plant extract. Histological analysis revealed alterations in the testis, epididymis, and prostate of the AS-exposed groups, including necrosis of seminiferous tubule epithelium and Leydig cells in the testis, and basal epithelial necrosis in the epididymis and prostate. Aluminum levels increased in all organs studied, while levels of zinc, copper, iron, and manganese decreased, showing a negative and significant correlation with aluminum levels. The aqueous extract of S. tectorum demonstrated a protective effect on certain studied biomarkers in male rats affected by aluminum exposure.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Sempervivum tectorum Extract on Some Biomarkers of Reproductive Function and Levels of Some Trace Elements in Male Rats Exposed to Aluminum

Muselin,

Dumitrescu,

Doma

et al. 2024

Animals

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“…Its liver-protecting and membrane-stabilizing effects, which have been attributed to antioxidant activity, have been described [22,23]. M. Florin et al described the protective effects of aqueous extract of S. tectorum on aluminum-induced oxidative stress in rat blood, indicating its good antioxidant properties [24]. Some articles have shown that leaf juice from S. tectorum contains polyphenol compositions [16] and high flavonoid content [25].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Extracts from Common Houseleek (Sempervivum tectorum) on the Metabolic Activity of Human Melanoma Cells WM-266-4

et al. 2021

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Human melanoma cells are known as one of the most aggressive cancer cells, and consequently, melanoma is one of the most incurable cancer diseases. There is intense activity in research and development of potential medicines for malignant diseases, including alternative forms of remedies. Therefore, the purpose of our work was testing extracts from the common houseleek (Sempervivum tectorum) grown in Slovenia to establish its impact on human melanoma cells. Namely, we wanted to verify if the extracts inhibit growth of malignant cells and their metabolic activity. Soxhlet, cold solvent, ultrasound, and supercritical extraction methods were applied to obtain S. tectorum extracts. Polyphenols and proanthocyanins content in acquired extracts was determined as well as their antioxidative potential. For a relevant comparison, Chinese (CHI) dried and Slovenian (SLO) lyophilized S. tectorum was used. Results showed that the highest contents of polyphenols and proanthocyanins were yielded from lyophilized material, which also had the highest antioxidative potential. The focus of our work was on analysis of possible inhibition effects of the extracts on human melanoma cells since no past studies were found regarding the possible effects of S. tectorum on metabolic activity of WM-266-4. We established that in a 24-h incubation period, the extracts inhibited metabolic activity of the cells at their concentrations of 20, 10, 4, 2, 1, 0.2, and 0.02 mg/mL. Extract obtained from SLO S. tectorum (ultrasound extraction with acetone as a solvent), which showed promising results of inhibitory effect on the mentioned cells, was further described since the local plant was the focus of our study. CHI S. tectorum extract (Soxhlet extraction with ehtanol:water mixture = 1:1 as a solvent) showed the highest inhibitory effect on human melanoma cells WM-266-4, although both obtained extracts are suitable for their growth inhibition of this specific cell line. Our results suggest inhibitory ability of S. tectorum extracts on the metabolic activity of WM-266-4 metastatic cell line, suggesting their potential use as an anticancer agent.

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“…Chromatographically purified fractions of S. tectorum L. in which rare natural components have been identified-monosaccharide sedoheptulose and polyalcohol 2-C-methyl-D-erythritol, as well as known organic acids and flavonoids, demonstrate the therapeutic effects of the plant in the healing of wounds [15]. Sempervivum tectorum extracts have been used to normalize oxidative stress biomarkers in the blood of rats [16].…”

Section: Introductionmentioning

confidence: 99%

“…Although several pharmacological properties have been reported for the leaves of Sempervivum tectorum L., a complete characterization of the extracts is not yet available. Therefore, only the polyphenolic contents and, in part, the antioxidant activities have been studied [16,18].…”

Section: Introductionmentioning

confidence: 99%

Optical Properties and Antioxidant Activity of Water-Ethanolic Extracts from Sempervivum tectorum L. from Bulgaria

et al. 2021

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Sempervivum tectorum L. is an evergreen plant belonging to a large family of the Crassulaceae. The fresh juice of the plant is used as a folk remedy almost exclusively for external purposes. The combination of several instrumental methods—transmission and fluorescence spectroscopy, ICP-MS spectrometry, and assays for the assessment of antioxidant activities were used for the characterization of water-ethanolic extracts from the leaves of Sempervivum tectorum L. with different polarities (ethanol content in the extracts varied between 10% and 95%). The highest total phenolic content was found in the 50% ethanol extract—0.84 ± 0.08 mg GAE/mL. Furthermore, this extract also possessed the highest antioxidant potential evaluated by DPPH and FRAP assays—7.02 ± 0.71 mM TE/mL and 6.15 ± 0.25 mM TE/mL, respectively. High correlation coefficients were found between the total phenolic contents and the antioxidant activities of water-ethanolic extracts from Sempervivum tectorum L. The same is true for the strong relationship between the phenolic contents and the concentrations of Na and K. Most likely, the bioavailable species of elements such as Fe, Zn, Ca, and Mg are mostly aqueous soluble. For all the studied extracts, the toxic element (As, Cd, Pb, Tl, Hg) contents are very much below the permissible limits for pharmaceutical products. On the contrary, the concentrations of compounds such as β-carotene and chlorophyll increase with the increase in ethanol in the extract. Results from this study may be used for the preliminary prognosis of pharmaceutical applications of extracts from Sempervivum tectorum L.

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Protective Effects of Aqueous Extract of <i>Sempervivum tectorum</i> L (<i>Crassulaceae</i>) on Aluminium-Induced Oxidative Stress in Rat Blood

Abstract: (CAT, 49.59 %, p < 0.001), glutathione (GSH, 13.49 %, p < 0.001) and glutathione reductase 23.08 %, p < 0.0001)

Cited by 15 publications

References 23 publications

Effect of Sempervivum tectorum Extract on Some Biomarkers of Reproductive Function and Levels of Some Trace Elements in Male Rats Exposed to Aluminum

Effect of Sempervivum tectorum Extract on Some Biomarkers of Reproductive Function and Levels of Some Trace Elements in Male Rats Exposed to Aluminum

The Influence of Extracts from Common Houseleek (Sempervivum tectorum) on the Metabolic Activity of Human Melanoma Cells WM-266-4

Optical Properties and Antioxidant Activity of Water-Ethanolic Extracts from Sempervivum tectorum L. from Bulgaria

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