Protective effects and mechanisms of mogroside V on LPS-induced acute lung injury in mice

Abstract

Protective effects and mechanisms of mogroside V on LPS-induced acute lung injury in mice, Pharmaceutical Biology, 52:6, 729-734, DOI: 10.3109/13880209.2013 Materials and methods:Female BALB/c mice were treated with commercial mogroside V (2.5, 5 and 10 mg/kg) for 1 h prior to intranasal injection of LPS (10 mg in 50 ml). After 12 h, airway inflammation in the ALI model was determined by the wet/dry weight (W/D) ratio, myeloperoxidase (MPO) activity of lung tissue, leukocyte recruitment and cytokine levels in the bronchoalveolar lavage fluid (BALF). Additionally, lung tissue was examined by histology and western blotting to investigate the changes in pathology and the signalling in the presence and absence of mogroside V. Results: Mogroside V at 5 and 10 mg/kg inhibited airway inflammation induced by LPS as measured by the decrease in the histological changes (44 and 67.3% reduction in lung injury score, respectively), a 28.9 and 55.3% reduction in lung MPO activity, and inflammatory cell counts, interleukin-1b (IL-1b, 382 and 280 pg/ml, respectively), IL-6 (378 and 232 pg/ml, respectively) and tumor necrosis factor-a (TNF-a, 12.5 and 7.8 ng/ml, respectively) levels in the BALF. Additionally, mogroside V treatment reduced the activation of cyclooxygenase 2 (COX-2), inducible NO synthase (iNOS), and the nuclear factor (NF)-kB. Discussions and conclusions: Together, these data suggest that mogroside V has the potential to protect against LPS-induced airway inflammation in a model of ALI.

Standardized myrtol attenuates lipopolysaccharide induced acute lung injury in mice

“…consistent with those of previous studies (Shi et al 2014;Zhang et al 2014). However, the events induced by LPS were reversed by pretreatment with standardized myrtol.…”

Therapeutic Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury Mice

“…Among them, sepsis is the major etiology for ALI development, wherein Gram-negative bacteria are a prominent cause25. LPS, the main component of the outer membranes of Gram-negative bacteria, is the most important antigen that promotes the development of ALI26. Therefore, the LPS-induced animal models could highlight ways to explore mechanisms of multiple diseases and provide useful information on the discovery of novel biomarkers and drug targets27.…”

Therapeutic Potential of Medicinal Plants and Their Constituents on Lung Inflammatory Disorders

“…This inhibitory action was mediated by the inhibition of MAPK and NF-κB pathways. Another triterpene derivative, mogroside V (35), reduced lung inflammation by the downregulation of COX-2 and iNOS via inhibiting NF-κB activation (Shi et al ., 2014). The famous diterpenoid, triptolide (57) from Trypterygium wilfordii , was also shown to inhibit LPS-induced lung inflammation at concentrations as low as 1 mg/kg via intraperitoneal injection (Wei and Huang, 2014).…”