Protective effect of Yiguanjian decoction against DNA damage on concanavalin A-induced liver injury mice model

Tian, Mengxi; Liu, Wenlan; You, Hongjie; Zhao, Qingchuan; Ouyang, Luo-Dan; Gao, Biane; Zhang, Xin; Niancong, Che

doi:10.1016/s0254-6272(16)30064-4

Cited by 15 publications

(2 citation statements)

References 9 publications

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“…The GDFMD comprises Reynoutria multiflora (Thunb. ), Lycium barbarum L., Panax notoginseng (Burkill) F.H.Chen, Curcuma aromatica Salisb., Smilax glabra Roxb., Bupleurum chinense DC, and Paeonia lactiflora Pall., which has been reported to be effective in treating WD-related liver disease [13,31,32]. However, the underlying biological processes that mediate this therapeutic effect remain to be identified.…”

Section: Discussionmentioning

confidence: 99%

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Tang

Zhao

Zhang

et al. 2023

Mediators of Inflammation

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Background. Gandoufumu decoction (GDFMD) is a traditional Chinese medicine that has been widely used to treat Wilson’s disease (WD) liver damage patients. However, its specific molecular mechanism currently remains unclear. Autophagy as a key contributor to WD liver damage has been intensely researched in the recent years. Therefore, the aim of this present study is to explore the effect of GDFMD on autophagy in WD liver damage, and the final purpose is to provide scientific evidence for GDFMD treatment in WD liver damage. Methods. The molecular mechanisms and autophagy-related pathways of GDFMD in the treatment of WD liver damage were predicted using network pharmacology. Copper assay kit was used to determine copper content in serum. Enzyme-linked immunosorbent assay (ELISA) was utilized to quantify serum levels of liver enzymes and oxidative stress-related indicators. Hematoxylin-eosin (HE), Masson, and Sirius red staining were used for the characterization of liver pathological changes. Transmission electron microscopy, immunofluorescence, and Western blot analyses were used to evaluate autophagy activity. The impact of the GDFMD on typical autophagy-related pathway (PI3K/Akt/mTOR pathway) molecules was also assessed via Western blot analysis. Results. GDFMD effectively attenuated serum liver enzymes, oxidative stress, autophagy, and degree of hepatic histopathological impairment and reduced serum copper content. Through network pharmacological approaches, PI3K/Akt/mTOR pathway was identified as the typical autophagy-related pathway of GDFMD in the treatment of WD liver damage. Treatment with GDFMD activated the PI3K/Akt/mTOR pathway, an effect that was able to be counteracted by LY294002, a PI3K antagonist or Rapa (rapamycin), an autophagy inducer. Conclusions. GDFMD imparted therapeutic effects on WD through autophagy suppression by acting through the PI3K/Akt/mTOR pathway.

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Section: Discussionmentioning

confidence: 99%

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Tang

Zhao

Zhang

et al. 2023

Mediators of Inflammation

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“…], Gouqizi [ Lycium barbarum L.], and Chuanlianzi [ Melia azedarach L.]. Research indicates the hepatoprotective, anti-fibrogenic, anti-angiogenesis effects of YGJ in hepatic injury mice models (Tian et al, 2016). In rat model of liver fibrosis, YGJ was found to alleviate the hepatic collagen hyperplasia and deteriorated hepatic function (Tao et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparative Network Pharmacology Analysis of Classical TCM Prescriptions for Chronic Liver Disease

Chen

Wang

et al. 2019

Front. Pharmacol.

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Chronic liver disease (CLD) has become a major global health problem while herb prescriptions are clinically observed with significant efficacy. Three classical Traditional Chinese Medicine (TCM) formulae, Yinchenhao Decoction (YCHT), Huangqi Decoction (HQT), and Yiguanjian (YGJ) have been widely applied in China to treat CLD, but no systematic study has yet been published to investigate their common and different mechanism of action (MOA). Partial limitation may own to deficiency of effective bioinformatics methods. Here, a computational framework of comparative network pharmacology is firstly proposed and then applied to herbal recipes for CLD disease. The analysis showed that, the three formulae modulate CLD mainly through functional modules of immune response, inflammation, energy metabolism, oxidative stress, and others. On top of that, each formula can target additional unique modules. Typically, YGJ ingredients can uniquely target the ATP synthesis and neurotransmitter release cycle. Interestingly, different formulae may regulate the same functional module in different modes. For instance, YCHT and YGJ can activate oxidative stress-related genes of SOD family while HQT are found to inhibit SOD1 gene. Overall, our framework of comparative network pharmacology proposed in our work may not only explain the MOA of different formulae treating CLD, but also provide hints to further investigate the biological basis of CLD subtypes.

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Total flavonoids from Tetrastigma hemsleyanum ameliorates inflammatory stress in concanavalin A-induced autoimmune hepatitis mice by regulating Treg/Th17 immune homeostasis

Peng

Lou

et al. 2019

Inflammopharmacol

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Protective effect of Yiguanjian decoction against DNA damage on concanavalin A-induced liver injury mice model

Cited by 15 publications

References 9 publications

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Comparative Network Pharmacology Analysis of Classical TCM Prescriptions for Chronic Liver Disease

Total flavonoids from Tetrastigma hemsleyanum ameliorates inflammatory stress in concanavalin A-induced autoimmune hepatitis mice by regulating Treg/Th17 immune homeostasis

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