Protective effect of prednisolone on ischemia-induced liver injury in rats

Abstract

AIM:To investigate the effects of prednisolone on cell membrane bleb formation, calpain μ activation and talin degradation during hepatic ischemia-reperfusion injury in rats. METHODS:The hilar area of the left lateral and median lobes of rat liver (68%) was clamped for 60 min and followed by 120 min reperfusion. Prednisolone was administered at 1.0, 3.0, or 10 mg/kg at 30 min before ischemia. In addition to biochemical and microscopic analyses, activation of calpain μ was determined using specific antibodies against the intermediate (activated) form of calpain μ. Degradation of talin was also studied by Western blotting. RESULTS: In the control and prednisolone (1.0 mg/kg) groups, serum aspartate transaminase (AST) and alanine transaminase (ALT) level were elevated, and cell membrane bleb formation was observed after 120 min of reperfusion. Moreover, calpain μ activation and talin degradation were detected. Infusion of prednisolone at 3.0 or 10 mg/kg significantly suppressed serum AST and ALT, and prevented cell membrane bleb formation. At 10 mg/kg, prednisolone markedly suppressed calpain μ activation and talin degradation. CONCLUSION: Prednisolone can suppress ischemiareperfusion injury of the rat liver. Its cytoprotective effect is closely associated with the suppression of calpain μ activation and talin degradation.

Corticosteroid effects on ventilator-induced diaphragm dysfunction in anesthetized rats depend on the dose administered

“…Interestingly, our results reveal that administration of a relatively low dose of methylprednisolone (5 mg/kg, corresponding ~0.8 mg/kg in humans [19]) exacerbates the CMV-induced diaphragm dysfunction, whereas a higher dose (30 mg/kg, corresponding ~5 mg/kg in humans [19]) completely protected the diaphragm against VIDD. The dose-depending effect of corticosteroids are in agreement with previous studies [7,8,31]. Our finding of a negative correlation between calpain activity and either diaphragmatic force production or diaphragm fiber CSA further supports the notion that calpain activation plays an important role in CMV-induced diaphragmatic atrophy and contractile dysfunction.…”

Determinants of the clinical outcome of patients with severe acute exacerbation of chronic hepatitis B virus infection

“…Other mechanisms of the effects of CS in AE may be related to the prevention of endotoxin-induced secondary liver injury [35], prevention of cytolysis of ballooned hepatocytes by stabilization of the lysosomal membrane [36], and improvement of the functional activity of the remaining hepatocytes [37]. Other studies showed that the preferential increase in the number of HBV-specific CD8 T and CD4 T cells is associated with viral control rather than liver damage [38,39].…”

Anti-CD163-dexamethasone protects against apoptosis after ischemia/reperfusion injuries in the rat liver

“…Steroids induce an anti-inflammatory effect by providing negative feedback on immunological stimulation, stabilizing cell membrane integrity by inhibition of lysosome peroxidation and through regulation of gene expression pathways [27,28] . Corticosteroids seem to have a positive impact on the extent of IRI in the liver through an anti-apoptotic effect [29–33] .…”