Protective effect of nutritional supplementation of zinc-sulfate against cisplatin-induced spermatogonial and testicular dysfunctions in adult male Sprague-Dawley rats

Adelakun, Sunday Aderemi; Ogunlade, Babatunde; Fidelis, Obinna P.; Omotoso, O

doi:10.1016/j.endmts.2021.100116

Cited by 7 publications

(5 citation statements)

References 63 publications

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“…In addition, CIS mitigates testosterone production via an ROS-inhibited P450 side-chain cleavage enzyme [ 26 ]. In accordance with several reports [ 14 , 22 , 27 ], impaired oxidative status in testicular tissues was presented in this work by a significant elevation in MDA content, a marker of lipid peroxidation, and total nitrite levels. In cases of increased oxidative stress, the polyunsaturated phospholipids of the cell membrane are oxidized, generating MDA.…”

Section: Discussionsupporting

confidence: 93%

“…The histopathological findings that showed desquamated and disorganized germinal cells as well as degeneration in germinal cells confirmed the deterioration of sperm quality indices in rats administered CIS alone. These observed effects of CIS were consistent with the results of previous studies [ 4 , 5 , 14 , 15 , 22 ]. However, pretreatment with TDF, PTX, and three doses of Cilo restored the loss of testicular weight and enhanced the number and motility of epididymal sperm, as well as serum testosterone levels, compared to the CIS group.…”

Section: Discussionsupporting

confidence: 93%

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Mechanistic Protective Effect of Cilostazol in Cisplatin-Induced Testicular Damage via Regulation of Oxidative Stress and TNF-α/NF-κB/Caspase-3 Pathways

Othman

Habib

Zahran

et al. 2023

IJMS

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Despite being a potent anticancer drug, cisplatin has limited applicability due to its adverse effects, such as testicular damage. Consequently, reducing its toxicity becomes necessary. In this study, a selective phosphodiesterase-3 inhibitor, cilostazol, which is used to treat intermittent claudication, was examined for its ability to abrogate cisplatin-induced testicular toxicity. Its ameliorative effect was compared to that of two phosphodiesterase inhibitors, tadalafil and pentoxifylline. The study also focused on the possible mechanisms involved in the proposed protective effect. Cisplatin-treated rats showed a significant decrease in sperm number and motility, serum testosterone, and testicular glutathione levels, as well as a significant elevation in malondialdehyde, total nitrite levels, and the protein expression of tumor necrosis factor-alpha, nuclear factor-kappa β, and caspase-3. These outcomes were confirmed by marked testicular architecture deterioration. Contrary to this, cilostazol, in a dose-dependent manner, showed potential protection against testicular toxicity, reversed the disrupted testicular function, and improved histological alterations through rebalancing of oxidative stress, inflammation, and apoptosis. In addition, cilostazol exerted a more pronounced protective effect in comparison to tadalafil and pentoxifylline. In conclusion, cilostazol ameliorates cisplatin-induced testicular impairment through alteration of oxidative stress, inflammation, and apoptotic pathways, offering a promising treatment for cisplatin-induced testicular damage.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Mechanistic Protective Effect of Cilostazol in Cisplatin-Induced Testicular Damage via Regulation of Oxidative Stress and TNF-α/NF-κB/Caspase-3 Pathways

Othman

Habib

Zahran

et al. 2023

IJMS

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“…A similar result with A. nodifera shell powder as a NAB was obtained at a dose of 3.3 g/day and ZnSO 4 0.45 mg/kg for 35 days for Bangkok chickens [17]. According to Adelakun et al [77], the ZnSO 4 treatment in rats for 56 days did not affect the DST compared to the controls. In addition, ZnSO 4 treatment to mice for 30 days did not affect the DST compared to the controls [78].…”

Section: Testicular Weight and Dst 56 Days Post-treatmentsupporting

confidence: 66%

“…According to Adelakun et al . [ 77 ], the ZnSO 4 treatment in rats for 56 days did not affect the DST compared to the controls. In addition, ZnSO 4 treatment to mice for 30 days did not affect the DST compared to the controls [ 78 ].…”

Section: Discussionmentioning

confidence: 97%

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

et al. 2023

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Background and Aim: Pelung cockerels (Gallus gallus gallusGallus gallus gallus [Linnaeus, 1758]) are different from other native cockerels in that they have a long and unique voice, in addition to their tall, large, and sturdy body with a relatively heavy body weight (BW). The sound quality of pelung cockerels is affected by the structure of the syrinx and their large and strong chest muscles. The performance of the chest muscles, and subsequently its voice, is influenced by the hormone testosterone. The shell of blood clams (Anadara granosa Linnaeus, 1758), a saltwater bivalve is known to contain a natural aromatase blocker (NAB) capable of blocking the aromatase enzyme from converting testosterone to estradiol. This generates consistently high levels of testosterone. This study aimed to determine the effect of blood clam shell powder (BCSP) as an NAB on the growth, pectoralis muscle performance, and testes of pelung cockerels. Materials and Methods: The study design was a completely randomized design, with 16 pelung cockerels aged 40–56 weeks divided into four treatment groups: T0 (control); T1 (BCSP [A. granosa] 0.9 mg/kg BW); T2 (zinc sulfate [ZnSO4] 0.9 mg/kg BW); and T3 (testosterone 3 mg/day). The animals were acclimatized for 7 days and then given dietary treatments for 56 days. The measurement of the comb, wattle, and chest circumference (CC) of pelung cockerels was performed on days 0, 14, 28, 42, and 56. At the end of the treatment, the pelung cockerels were sacrificed and the data of the pectoralis muscle weight (PMW), testis weight (TW), and area of the pectoralis muscle (APM) were measured. Samples of pectoralis muscle and testes were taken and fixed in 10% neutral buffer formalin for histology. The proliferating cell nuclear antigen (PCNA) was identified by immunohistochemical staining. To measure fascicle area (FA), myofiber area (MA), and enumerate, the fascicle myofibers (NM) histology preparations were stained with hematoxylin and eosin (H and E). Testicular preparations were stained with H and E to measure the diameter of the seminiferous tubules (DST) using ImageJ software. Results: The growth performance on day 56 showed significantly (p < 0.05) higher differences of CC in T1 compared to T2 and T0, in T1 and T3 compared to T0, and in T3 and T2 compared to T0. Pectoralis muscle results, that is, FA, NM, MA, and PCNA-positive cells, showed that cockerels on treatment T3 had significantly higher results than other treatments, T1 was significantly different from T2 and T0, and T2 was significantly different from T0. In addition, the TW and DST measurement of cockerels on treatment T3 were significantly reduced (p < 0.05) than the other treatment groups. Conclusion: The oral administration of BCSP in the role of a NAB at a dose of 0.9 mg/kg BW for 56 days improved the growth performance and pectoralis muscle, especially the CC, FA, NM, MA, and PCNA-positive cells parameters, but did not affect the PMW, APM, and testis of pelung cockerels. The administration of testosterone at 3 mg/day for 56 days contributed to the decrease in TW and DST, as well as atrophy of the seminiferous tubules of pelung cockerels. Keywords: growth performance, muscle, natural aromatase blocker, pelung, testis.

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“…Meanwhile, the Lee index for each animal was calculated by multiplying the complete expression by 1000 and dividing the cube root of the body weight (g) by the naso-anal length (Cm) i.e [LI = body weight (g) 1/3 x1000/body length (cm)]. If the Lee index score was greater than 310, rats were termed fat [ 44 , 45 ].…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effects of aqueous extract of bioactive active component of Ageratum conyzoides on the ovarian-uterine and hypophysis-gonadal axis in rat with polycystic ovary syndrome: Histomorphometric evaluation and biochemical assessment

et al. 2022

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Protective effect of nutritional supplementation of zinc-sulfate against cisplatin-induced spermatogonial and testicular dysfunctions in adult male Sprague-Dawley rats

Cited by 7 publications

References 63 publications

Mechanistic Protective Effect of Cilostazol in Cisplatin-Induced Testicular Damage via Regulation of Oxidative Stress and TNF-α/NF-κB/Caspase-3 Pathways

Mechanistic Protective Effect of Cilostazol in Cisplatin-Induced Testicular Damage via Regulation of Oxidative Stress and TNF-α/NF-κB/Caspase-3 Pathways

Growth, pectoralis muscle performance, and testis of pelung cockerels (Gallus gallus gallus [Linnaeus, 1758]) supplemented with blood clam shell powder (Anadara granosa [Linnaeus, 1758])

Therapeutic effects of aqueous extract of bioactive active component of Ageratum conyzoides on the ovarian-uterine and hypophysis-gonadal axis in rat with polycystic ovary syndrome: Histomorphometric evaluation and biochemical assessment

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