Protective effect of Lycium barbarum polysaccharides on oxidative damage in skeletal muscle of exhaustive exercise rats

Ai-jun, Niu; Wu, Jian; Yu, Dan; Wang, Ru

doi:10.1016/j.ijbiomac.2008.02.003

Cited by 77 publications

(55 citation statements)

References 24 publications

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“…Consistent with previous studies [12,13], LBPs showed an anti-oxidant capacity in PC-12 cells under H 2 O 2 -stimulation. We also proved that LBPsinduced reduction of oxidative stress was reversed by miR-194 overexpression while was augmented by miR-194 inhibition, indicating that down-regulation of miR-194 might be a rational explanation for the anti-oxidant capacity of LBPs.…”

Section: Discussionsupporting

confidence: 92%

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Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells

Niu

Jin

Niu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Currently, scientists attempt to improve outcome of spinal cord injury (SCI) via reducing secondary injury during SCI. Oxidative stress is critical for pathophysiology of secondary damage, thus we mainly focused on the anti-oxidant effects of Lycium barbarum polysaccharides (LBPs) on PC-12 and SH-SY5Y cells as well as the underlying mechanisms. Methods: Oxidative stress was induced by H₂O₂ stimulation. Effects of LBPs on cell viability, apoptosis, and expression of proteins associated with apoptosis and autophagy in H₂O₂-induced cells were assessed by CCK-8 assay, flow cytometry assay and Western blot analysis, respectively. Then, expression of miR-194 was determined by qRT-PCR. Expression of miR-194 was dysregulated, and whether LBPs affected H₂O₂-treated cells through modulating miR-194 was verified. The expression of key kinases in the PI3K/AKT pathway and the intracellular levels of ROS and NO were testified by Western blot analysis and flow cytometry with fluorescent probes. Results: H₂O₂-induced decrease of cell viability and increases of apoptosis and autophagy in PC-12 cells were mitigated by LBPs treatment. Next, we found that miR-194 expression was both down-regulated by LBPs treatment in PC-12 and SH-SY5Y cells. More experiments consolidated that influence of LBPs on H₂O₂-treated cells was reversed by miR-194 overexpression while was augmented by miR-194 inhibition. LBPs elevated the phosphorylated levels of PI3K and AKT and reduced levels of ROS and NO through miR-194. Conclusion: LBPs alleviated H₂O₂-induced decrease of cell viability, and increase of apoptosis and autophagy through down-regulating miR-194. Moreover, LBPs activated the PI3K/AKT pathway and reduced oxidative stress through miR-194.

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Section: Discussionsupporting

confidence: 92%

“…For example, a previous study has stated that oxidative stress induced by hepatic cadmium in rats is reduced by LBPs [12]. Likewise, oxidative stress induced by exhaustive exercise of skeletal muscle in rats is also ameliorated by LBPs [13]. Recently, the neuroprotective role of LBPs has been reported [14].…”

Section: Introductionmentioning

confidence: 99%

Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells

Niu

Jin

Niu

et al. 2018

Cell Physiol Biochem

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show abstract

“…Lipid peroxidation causes increased membrane permeability, resulting in loss of cytosolic proteins. The administration of L. barbarum with a high content of polysaccharides improved muscle antioxidant activity (Niu et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

Untitled

2017

PM3

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“…Lipid peroxidation can cause fluidity loss and permeability increase of membranes, leading to the loss of cytosolic proteins [33]. Inhibition of lipid peroxidation is essential for maintaining normal cell function [34].…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides from Polygonatum odoratum strengthen antioxidant defense system and attenuate lipid peroxidation against exhaustive exercise-induced oxidative stress in mice

Yang¹,

Zhang²

2017

Trop. J. Pharm Res

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Protective effect of Lycium barbarum polysaccharides on oxidative damage in skeletal muscle of exhaustive exercise rats

Cited by 77 publications

References 24 publications

Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells

Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells

Untitled

Polysaccharides from Polygonatum odoratum strengthen antioxidant defense system and attenuate lipid peroxidation against exhaustive exercise-induced oxidative stress in mice

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