Protective effect of Jie-Geng-Tang against Staphylococcus aureus induced acute lung injury in mice and discovery of its effective constituents

Liu, Yining; Hong, Zhuping; Qian, Jing; Wang, Yi; Wang, Shufang

doi:10.1016/j.jep.2019.112076

Cited by 20 publications

(10 citation statements)

References 23 publications

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“…In brief, the names search for bioactive compounds of Glycyrrhiza glabra which has been documented for their antitumor, antimicrobial, antiviral and immunoregulatory efficacies viz. glycyrrhizinic acid (CID_14982), licochalcone A (CID_5318998), licochalcone B (CID_5318999), licochalcone C (CID_9840805), licochalcone D (CID_10473311), licochalcone E (CID_46209991), licochalcone F (CID_44130137), licochalcone G (CID_49856081), glyasperin A (CID_5481963), glyasperin B (CID_480784), glyasperin C (CID_480859), glyasperin D (CID_480860), isoliquiritinapioside (CID_6442433), 1-methoxyphaseollidin (CID_480873), dehydroglyasperin C (CID_480775), kanzonol Q (CID_11253965), liquiritin (CID_503737), hedysarimcoumestan B (CID_11558452), 5,6,7,8-tetrahydro-2,4-dimethylquinoline (CID_5321849), 5,6,7,8-tetrahydro-4-methylquinoline (CID_185667) (Bode & Dong, 2015;Jiang et al, 2020;Kim & Ma, 2018;Liu et al, 2019;Prajapati & Patel, 2015;Yang et al, 2017); was carried out in PubChem database. The resulting 3D structures in sdf file format were directly used in the generation of input pdbqt files for Autodock vina.…”

Section: Molecular Docking Simulationmentioning

confidence: 99%

“…In brief, glycyrrhizic acid has been reported for its efficacy against HIV-1 chronic hepatitis C virus, coxsackievirus A16 and enterovirus 71 and Kaposi sarcoma-associated herpesvirus (Curreli et al, 2005;De Clercq, 2000;Sabouri Ghannad et al, 2014;Wang et al, 2013). The licochalcone A and isoliquiritigenin has been effective in preventing the acute lung injury induced of pathogenic origin (Liu et al, 2019). Liquiritin has also been reported for their anti-viral potential against HIV (Grienke et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Identification of bioactive compounds fromGlycyrrhiza glabraas possible inhibitor of SARS-CoV-2 spike glycoprotein and non-structural protein-15: a pharmacoinformatics study

Sinha

Prasad

Islam

et al. 2020

Journal of Biomolecular Structure and Dynamics

114

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Aim: In view of the strong immunomodulatory and antiviral activity of andrographolide and its derivative, the present study aimed to investigate the binding a nities of andrographolide and its derivative 14deoxy-11,12-didehydroandrographolide with 3 major targets of COVID-19 i.e. 3CLpro, PLpro and spike protein followed by their gene-set enrichment analysis with special reference to immune modulation. Materials and methods: SMILES of the compounds were retrieved from DigepPred database and the proteins identi ed were queried in STRING to evaluate the protein-protein interaction and modulated pathways were identi ed concerning the KEGG database. Drug-likeness and ADMET pro les were evaluated using MolSoft and admet SAR 2.0, respectively. Molecular docking was carried using autodock 4.0. Results: Andrographolide and 14-Deoxy-11,12-didehydroandrographolide were predicted to have a high binding a nity with papain-like protease i.e.-6.7 kcal/mol and-6.5 kcal/mol, respectively while they interact with equal binding energies with 3clpro (-6.8 kcal/mol) and spike protein (-6.9 kcal/mol). Network pharmacology analysis revealed that both compounds modulated the immune system through the regulation of chemokine signaling pathway, Rap1 signaling pathway, Cytokine-cytokine receptor interaction, MAPK signaling pathway, NF-kappa B signaling pathway, Rassignaling pathway, p53 signaling pathway, HIF-1 signaling pathway, and Natural killer cell-mediated cytotoxicity. Although the 14deoxy-11,12-didehydroandrographolide scored higher drug-likeness character, it showed less potency to interaction with targeted proteins of COVID-19. Conclusion: The study suggests the strong interaction of the andrographolide and its derivative 14-deoxy-11,12-didehydroandrographolide against target proteins associated with COVID-19. Further, network pharmacology analysis elucidated the different pathways of immunomodulation. However, clinical research should be conducted to con rm the current ndings.

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Section: Molecular Docking Simulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of bioactive compounds fromGlycyrrhiza glabraas possible inhibitor of SARS-CoV-2 spike glycoprotein and non-structural protein-15: a pharmacoinformatics study

Sinha

Prasad

Islam

et al. 2020

Journal of Biomolecular Structure and Dynamics

114

View full text Add to dashboard Cite

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“…A single stock of the virus was used in this study. Bacteria were grown in tryptic soy broth medium and quantified according to the OD600-based bacterial growth curve and colony forming unit (CFU) assays [30] , [31] .…”

Section: Methodsmentioning

confidence: 99%

Babaodan controls excessive immune responses and may represent a cytokine-targeted agent suitable for COVID-19 treatment

Qian

et al. 2021

Biomedicine & Pharmacotherapy

Self Cite

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It has become evident that the actions of pro-inflammatory cytokines and/or the development of a cytokine storm are responsible for the occurrence of severe COVID-19 during SARS-CoV-2 infection. Although immunomodulatory mechanisms vary among viruses, the activation of multiple TLRs that occurs primarily through the recruitment of adapter proteins such as MyD88 and TRIF contributes to the induction of a cytokine storm. Based on this, controlling the robust production of pro-inflammatory cytokines by macrophages may be applicable as a cellular approach to investigate potential cytokine-targeted therapies against COVID-19. In the current study, we utilized TLR2/MyD88 and TLR3/TRIF co-activated macrophages and evaluated the anti-cytokine storm effect of the traditional Chinese medicine (TCM) formula Babaodan (BBD). An RNA-seq-based transcriptomic approach was used to determine the molecular mode of action. Additionally, we evaluated the anti-inflammatory activity of BBD in vivo using a mouse model of post-viral bacterial infection-induced pneumonia and seven severely ill COVID-19 patients. Our study reveals the protective role of BBD against excessive immune responses in macrophages, where the underlying mechanisms involve the inhibition of the NF-κB and MAPK signaling pathways. In vivo, BBD significantly inhibited the release of IL-6, thus resulting in increased survival rates in mice. Based on limited data, we demonstrated that severely ill COVID-19 patients benefited from BBD treatment due to a reduction in the overproduction of IL-6. In conclusion, our study indicated that BBD controls excessive immune responses and may thus represent a cytokine-targeted agent that could be considered to treating COVID-19.

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“…Current therapies for ALI mainly focus on anti-infection using antipathogenic microorganism drugs, antiin ammation with glucocorticoids and respiratory support with oxygen therapy and/or mechanical ventilation and have disadvantages including development of opportunistic infection, immunode ciency, gastrointestinal hemorrhage and ventilator associated lung injury, which are vital reasons for high mortality of ALI/ARDS [1,3]. Traditional Chinese medicine (TCM) preparations such as Jie-Geng-Tang, Shufeng Jiedu Capsule and Dachengqi decoction have emerged as promising therapies for ALI in animal models due to their high e ciency, low-cost, easy accessibility and low adverse effect rate [4][5][6]. Shen-suyin (SSY, Jinsoin in Japanese, Samsoeum in Korean) is a traditional Chinese herb formula consisting of 13 kinds of Chinese herbs, which was rst recorded in the Prescriptions of the Bureau of Taiping People's Welfare Pharmacy in Song Dynasty in China.…”

Section: Introductionmentioning

confidence: 99%

Shen-su-yin, a Traditional Herbal Medicine, Attenuates Lipopolysaccharide-induced Acute Lung Injury in Rats Through Its Anti-inflammatory and Anti-oxidative Effects

Xiao

Zhang

et al. 2021

Preprint

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Most components of Shen-su-yin (SSY), an herbal formula, have anti-inflammatory and antioxidant activities. The present study was designed to investigate potential effects and mechanisms of SSY on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. 48 rats were randomly divided into 4 groups: control (Ctrl) group, LPS-induced ALI group, low- (SSY-LD) and high- (SSY-HD) dose SSY-treated ALI group. SSY was administered to SSY-treated rats immediately after LPS induction. After 24 hours, blood gas analysis and lactate determination were performed; and bronchoalveolar lavage fluid was collected for detecting protein concentration and levels of cytokines. Lung tissues were obtained for Western blot analysis, histopathological analysis, wet-to-dry weight ratio calculation and measurement of oxidative stress levels. SSY improved oxygenation index and mean arterial pressure, decreased levels of lactate and heart rate, alleviated lung histopathology indexes including lung injury score, wet-to-dry weight ratio and exudation of protein as well as inflammatory cells in ALI rats. Furthermore, SSY reduced levels of pro-inflammatory and oxidative mediums, while increasing levels of anti-inflammatory cytokine and anti-oxidative activity in lung tissues. SSY also suppressed NF-κB signalling pathway and further activated Keap1-Nrf2-ARE signalling pathway activated by LPS. Moreover, all the effects caused by SSY in the SSY-HD group were more encouraging than those in the SSY-LD group. The results indicate that the preventive use of SSY can alleviate ALI through the anti-inflammatory and antioxidant effects mediated by inhibition of NF-κB signalling pathway and activation of Keap1-Nrf2-ARE signalling pathway, and the effect of high dose is better.

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Protective effect of Jie-Geng-Tang against Staphylococcus aureus induced acute lung injury in mice and discovery of its effective constituents

Cited by 20 publications

References 23 publications

Identification of bioactive compounds fromGlycyrrhiza glabraas possible inhibitor of SARS-CoV-2 spike glycoprotein and non-structural protein-15: a pharmacoinformatics study

Identification of bioactive compounds fromGlycyrrhiza glabraas possible inhibitor of SARS-CoV-2 spike glycoprotein and non-structural protein-15: a pharmacoinformatics study

Babaodan controls excessive immune responses and may represent a cytokine-targeted agent suitable for COVID-19 treatment

Shen-su-yin, a Traditional Herbal Medicine, Attenuates Lipopolysaccharide-induced Acute Lung Injury in Rats Through Its Anti-inflammatory and Anti-oxidative Effects

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